Click here to switch to the map view.

The map label for this gene is folE

Identifier: 86749305

GI number: 86749305

Start: 2477826

End: 2478515

Strand: Direct

Name: folE

Synonym: RPB_2184

Alternate gene names: 86749305

Gene position: 2477826-2478515 (Clockwise)

Preceding gene: 86749303

Following gene: 86749306

Centisome position: 46.47

GC content: 67.39

Gene sequence:

>690_bases
ATGGACGCCAAGATCAAGCCGCTCCGCGCCGGCAAGTCCGCGGACGCCCGCACCGATTTTCAACCCGCCGAGCTCGACCC
GTCGGAATTCCTCGCTGCCGCGGTGCAGCCGGATCAGCCGCGGCCGTCGCGCGCCGAAGCCGAGGCGGCGGTGAAGACGC
TGCTGTCCTATATCGGCGAGAACACCGAGCGGGAGGGCCTGCTCGACACCCCGCGCCGGGTGGTCGAGGCCTATGACGAA
CTGTTCCAGGGCTATCACCAGTGCCCGGCCGAAGTGCTCGACCGCACCTTCGGCGAGACGGCCGGCTACGACGATTTCGT
GCTGGTGCGCGACATCTCCTTCACCTCGCATTGCGAACACCACGTCATGCCGTTCTACGGCAAGGCGCATATCGCCTACA
CGCCGGTCGAGCGCGTCGTCGGCCTGTCCAAGCTGGCGCGGCTGGTCGAGATTTTCGCCCGCCGGCTGCAGACCCAGGAG
CACCTCACCGCGCAGATCGCCGCGGCGATCGACGAGGTGCTGAAGCCGCGCGGCGTCGCGGTAATGATCGAGGCCGAGCA
CACCTGCATGTCGGTGCGCGGCATCGGCAAGCAGGGCGCCTCGACCTTCACCAGCCGCTACACCGGCATGTTCCGCGACA
ATCCGGCGGAGCAGGCGCGGTTCATGTCGATGATTCGCAACCGCGGTTAA

Upstream 100 bases:

>100_bases
CGCGGCGGGGTTCGCTGAAGGGATCAAGACGACCGGCGGCTGGCCGCCCGTCTGCCCGGTGACACATCCGGCCCGCAGGG
GCCGGTCGAACGGAGAGAAG

Downstream 100 bases:

>100_bases
GCCGCAGGATTGCGAGAGGAAAACGCTCGTGTGCTCATCTGCTGCCGCATCGCCCGCGCTCGATGATATCGAGGAGGGCA
TCGCGCTGCGTCCGAAATTC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MDAKIKPLRAGKSADARTDFQPAELDPSEFLAAAVQPDQPRPSRAEAEAAVKTLLSYIGENTEREGLLDTPRRVVEAYDE
LFQGYHQCPAEVLDRTFGETAGYDDFVLVRDISFTSHCEHHVMPFYGKAHIAYTPVERVVGLSKLARLVEIFARRLQTQE
HLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGIGKQGASTFTSRYTGMFRDNPAEQARFMSMIRNRG

Sequences:

>Translated_229_residues
MDAKIKPLRAGKSADARTDFQPAELDPSEFLAAAVQPDQPRPSRAEAEAAVKTLLSYIGENTEREGLLDTPRRVVEAYDE
LFQGYHQCPAEVLDRTFGETAGYDDFVLVRDISFTSHCEHHVMPFYGKAHIAYTPVERVVGLSKLARLVEIFARRLQTQE
HLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGIGKQGASTFTSRYTGMFRDNPAEQARFMSMIRNRG
>Mature_229_residues
MDAKIKPLRAGKSADARTDFQPAELDPSEFLAAAVQPDQPRPSRAEAEAAVKTLLSYIGENTEREGLLDTPRRVVEAYDE
LFQGYHQCPAEVLDRTFGETAGYDDFVLVRDISFTSHCEHHVMPFYGKAHIAYTPVERVVGLSKLARLVEIFARRLQTQE
HLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGIGKQGASTFTSRYTGMFRDNPAEQARFMSMIRNRG

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=180, Percent_Identity=48.8888888888889, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI4503949, Length=180, Percent_Identity=48.8888888888889, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI66932970, Length=148, Percent_Identity=49.3243243243243, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI66932972, Length=138, Percent_Identity=52.1739130434783, Blast_Score=144, Evalue=5e-35,
Organism=Escherichia coli, GI1788476, Length=168, Percent_Identity=38.6904761904762, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=47.2222222222222, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=47.7777777777778, Blast_Score=173, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24656782, Length=206, Percent_Identity=43.2038834951456, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24656796, Length=206, Percent_Identity=43.2038834951456, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24656791, Length=206, Percent_Identity=42.7184466019417, Blast_Score=160, Evalue=6e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_RHOP2 (Q2IY20)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485801.1
- ProteinModelPortal:   Q2IY20
- SMR:   Q2IY20
- STRING:   Q2IY20
- GeneID:   3909964
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2184
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   ARFMSMI
- ProtClustDB:   PRK09347
- BioCyc:   RPAL316058:RPB_2184-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 25545; Mature: 25545

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKIKPLRAGKSADARTDFQPAELDPSEFLAAAVQPDQPRPSRAEAEAAVKTLLSYIGE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
NTEREGLLDTPRRVVEAYDELFQGYHQCPAEVLDRTFGETAGYDDFVLVRDISFTSHCEH
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHCCC
HVMPFYGKAHIAYTPVERVVGLSKLARLVEIFARRLQTQEHLTAQIAAAIDEVLKPRGVA
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VMIEAEHTCMSVRGIGKQGASTFTSRYTGMFRDNPAEQARFMSMIRNRG
EEEECCHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAKIKPLRAGKSADARTDFQPAELDPSEFLAAAVQPDQPRPSRAEAEAAVKTLLSYIGE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
NTEREGLLDTPRRVVEAYDELFQGYHQCPAEVLDRTFGETAGYDDFVLVRDISFTSHCEH
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHCCC
HVMPFYGKAHIAYTPVERVVGLSKLARLVEIFARRLQTQEHLTAQIAAAIDEVLKPRGVA
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VMIEAEHTCMSVRGIGKQGASTFTSRYTGMFRDNPAEQARFMSMIRNRG
EEEECCHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA