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The map label for this gene is hisI

Identifier: 86749306

GI number: 86749306

Start: 2478544

End: 2478990

Strand: Direct

Name: hisI

Synonym: RPB_2185

Alternate gene names: 86749306

Gene position: 2478544-2478990 (Clockwise)

Preceding gene: 86749305

Following gene: 86749307

Centisome position: 46.49

GC content: 66.89

Gene sequence:

>447_bases
GTGTGCTCATCTGCTGCCGCATCGCCCGCGCTCGATGATATCGAGGAGGGCATCGCGCTGCGTCCGAAATTCGACGCGGC
CGGCCTCGTCACCTGCGTCACCACCGACGCGGGTTCAGGCGATGTCTTGATGGTGGCGCATATGAACGCGGAAGCGCTGG
AGAAAACCATCCAGAGCGGCGAGGCCTGGTACTACAGCCGCTCGCGCAAGCGTCTCTGGAAGAAGGGCGAGAGCTCCGGC
CACGTCCAGCGCGTCTTGGAGATGCGCATCGATTGCGACCAGGACGCGGTCTGGATCCGGGTCGACCAGGCCGGCGGCGC
CGCCTGCCACACCGGCCGCAAGTCCTGCTTCTACCGCCGCATCGACCGCGACGCCTCCGGCGAGCCGCTGCTGACCATGG
TCGACGCCGAGCGGCAGTTCGACCCGGACAAGGTCTACGGCAAGTAG

Upstream 100 bases:

>100_bases
TACACCGGCATGTTCCGCGACAATCCGGCGGAGCAGGCGCGGTTCATGTCGATGATTCGCAACCGCGGTTAAGCCGCAGG
ATTGCGAGAGGAAAACGCTC

Downstream 100 bases:

>100_bases
CGCCACGCGCTTCTTCCCGTCATTCCGGGGCGCTCGCGCAGCGAGCGAACCCGGAATCCCGAGCTGTTCATCGCGCGGCA
TTCCCAACCTCGAGATTCCG

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MCSSAAASPALDDIEEGIALRPKFDAAGLVTCVTTDAGSGDVLMVAHMNAEALEKTIQSGEAWYYSRSRKRLWKKGESSG
HVQRVLEMRIDCDQDAVWIRVDQAGGAACHTGRKSCFYRRIDRDASGEPLLTMVDAERQFDPDKVYGK

Sequences:

>Translated_148_residues
MCSSAAASPALDDIEEGIALRPKFDAAGLVTCVTTDAGSGDVLMVAHMNAEALEKTIQSGEAWYYSRSRKRLWKKGESSG
HVQRVLEMRIDCDQDAVWIRVDQAGGAACHTGRKSCFYRRIDRDASGEPLLTMVDAERQFDPDKVYGK
>Mature_148_residues
MCSSAAASPALDDIEEGIALRPKFDAAGLVTCVTTDAGSGDVLMVAHMNAEALEKTIQSGEAWYYSRSRKRLWKKGESSG
HVQRVLEMRIDCDQDAVWIRVDQAGGAACHTGRKSCFYRRIDRDASGEPLLTMVDAERQFDPDKVYGK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=90, Percent_Identity=43.3333333333333, Blast_Score=90, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319819, Length=90, Percent_Identity=37.7777777777778, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_RHOP2 (Q2IY19)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485802.1
- ProteinModelPortal:   Q2IY19
- SMR:   Q2IY19
- STRING:   Q2IY19
- GeneID:   3909965
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2185
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   PRK00051
- BioCyc:   RPAL316058:RPB_2185-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 16287; Mature: 16287

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSSAAASPALDDIEEGIALRPKFDAAGLVTCVTTDAGSGDVLMVAHMNAEALEKTIQSG
CCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHCC
EAWYYSRSRKRLWKKGESSGHVQRVLEMRIDCDQDAVWIRVDQAGGAACHTGRKSCFYRR
CCEEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHCCHHHHHHHH
IDRDASGEPLLTMVDAERQFDPDKVYGK
HCCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MCSSAAASPALDDIEEGIALRPKFDAAGLVTCVTTDAGSGDVLMVAHMNAEALEKTIQSG
CCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHCC
EAWYYSRSRKRLWKKGESSGHVQRVLEMRIDCDQDAVWIRVDQAGGAACHTGRKSCFYRR
CCEEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHCCHHHHHHHH
IDRDASGEPLLTMVDAERQFDPDKVYGK
HCCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA