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The map label for this gene is menE [H]

Identifier: 86749223

GI number: 86749223

Start: 2390044

End: 2391570

Strand: Direct

Name: menE [H]

Synonym: RPB_2102

Alternate gene names: 86749223

Gene position: 2390044-2391570 (Clockwise)

Preceding gene: 86749222

Following gene: 86749225

Centisome position: 44.83

GC content: 68.3

Gene sequence:

>1527_bases
ATGAATCTGTCCGAATGGCTCGCCGCGAGCGCACGGCTGCGGCCGTCCGCGCCGGCCTTGCTCACCGGCACCACGATCGA
GGCGGACTACGCGACGTTCGCGCAGCGCGCCGCCTCGTTCGCTGCAGCGCTGCAGCGCGACTACGGCATCGTCTCCGGCG
ACCGCGTCGCGCTGTTCGCGCATAATTGCACGCAATATCTCGAGGCACTGTACGGCATCTGGTGGGCGGGCGCGGTGGCG
GTGCCGATCAACGCCAAGCTGCACGGCAAGGAAGCGGCGTGGATCTGCAGCAATTCCGGCGCCAAGCTGGCGCTGATCTG
CGACGACACCGCGGACACTTTCAACGAGGCCGCGGGCGAATTGCCGGCCCGCATGGCGACGCTGGCGCTCGACAGCGACG
CCTACATTCGCGCCCGTAGCGGCGACGGGCCGGCGGCGCCGGCGGCGCGCGAGGACGGCGATCTCGCCTGGCTGTTCTAC
ACCTCCGGCACCACCGGCCGGCCGAAGGGGGTGATGCTCAGCCACGGCAATCTGATCGCGATGTCGCTGTGCTATTTGGC
CGATGTCGACACGGTGTCGTCCGATGACGCCGCGCTCTATGCCGCGCCGATCTCGCACGGTGCCGGGCTCTACAACATGA
TCCACACCCGGTTCGGCGCGCGTCACGTCGTGCCCGCCTCCAAGGGCTTCGACCCCGACGAGGTGCTGACGCTCGGCAAG
CAGCTCGGCAACGTCGCGATGTTCGCCGCGCCCACCATGGTGAAGCGGCTGGTCGAGGCCGCAAGGCGCCGCGGCGAGCG
CGGCGAGGGACTGCGCACCATCGTCTACGGCGGCGGCCCGATGTATCTCGCCGACATCCGCGACGCGCTCGACGTGATGG
GCCAGCGCTTCGTGCAGATCTACGGCCAGGGCGAATCGCCGATGGCGATCACGTCGCTGAAGCGCGAGTTGCACGCCGAT
GTCGATCATCCGCGCTATCTGCAGCGGCTGGCCTCGGTCGGCACCGCGCAGAGCGCGCTGTCGGTGCGGATCACCGGGCC
TGACGGCGAGGTGCTGCCGGCCGGCGAGACCGGCGAGATCGAGGCCAAGGGCCCGACCGTGATGCTCGGCTACTGGAACA
ATTCGGACGCCAACGCCGAGACGCTGAAAGACGGCTGGCTGCGCACCGGCGATGTCGGGCGCCTGGACGAGGACGGCTTT
CTCACGCTGTCGGACCGCTCCAAGGACGTGATCATCTCCGGCGGCACCAACATCTATCCGCGCGAAGTGGAAGAAGCGCT
GCTGACGCATCCCGCGGTGCGCGAGGTCTCGGCGATCGGCGTCGCCGATCCGGAATGGGGCGAGACCGTGGTCGCCTGTG
TGGTGCTGGCGGACGGATCGGAGCCCAGCGACACTGCGCTCGACGCGCATTGCCTCGCCGCCATCGCCCGCTTCAAGCGG
CCGAAGCGCTACGTCTATCTGGAAGCGTTGCCGAAGAACAATTACGGCAAGGTGCTGAAGACCGAGCTGCGCAAGATGGT
GACTTAG

Upstream 100 bases:

>100_bases
GACGCGGCAAAGACGTGGATGGCCGGGACAAGCCCGGCCATGACGGCAAGCGGGCCGGCAGTCGCGTCCCCATTGAACAC
TCGATTTGCGGGGGCTTGCG

Downstream 100 bases:

>100_bases
TCTCCGCCGTCATTGGGGCAGCGGCCCAGCCTATCCACGAGCGTCATCCTGAGGTGCCTGCCACGCCGCGTCGCGGCGTG
GCAGGCCTCGAAGGATGAGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNLSEWLAASARLRPSAPALLTGTTIEADYATFAQRAASFAAALQRDYGIVSGDRVALFAHNCTQYLEALYGIWWAGAVA
VPINAKLHGKEAAWICSNSGAKLALICDDTADTFNEAAGELPARMATLALDSDAYIRARSGDGPAAPAAREDGDLAWLFY
TSGTTGRPKGVMLSHGNLIAMSLCYLADVDTVSSDDAALYAAPISHGAGLYNMIHTRFGARHVVPASKGFDPDEVLTLGK
QLGNVAMFAAPTMVKRLVEAARRRGERGEGLRTIVYGGGPMYLADIRDALDVMGQRFVQIYGQGESPMAITSLKRELHAD
VDHPRYLQRLASVGTAQSALSVRITGPDGEVLPAGETGEIEAKGPTVMLGYWNNSDANAETLKDGWLRTGDVGRLDEDGF
LTLSDRSKDVIISGGTNIYPREVEEALLTHPAVREVSAIGVADPEWGETVVACVVLADGSEPSDTALDAHCLAAIARFKR
PKRYVYLEALPKNNYGKVLKTELRKMVT

Sequences:

>Translated_508_residues
MNLSEWLAASARLRPSAPALLTGTTIEADYATFAQRAASFAAALQRDYGIVSGDRVALFAHNCTQYLEALYGIWWAGAVA
VPINAKLHGKEAAWICSNSGAKLALICDDTADTFNEAAGELPARMATLALDSDAYIRARSGDGPAAPAAREDGDLAWLFY
TSGTTGRPKGVMLSHGNLIAMSLCYLADVDTVSSDDAALYAAPISHGAGLYNMIHTRFGARHVVPASKGFDPDEVLTLGK
QLGNVAMFAAPTMVKRLVEAARRRGERGEGLRTIVYGGGPMYLADIRDALDVMGQRFVQIYGQGESPMAITSLKRELHAD
VDHPRYLQRLASVGTAQSALSVRITGPDGEVLPAGETGEIEAKGPTVMLGYWNNSDANAETLKDGWLRTGDVGRLDEDGF
LTLSDRSKDVIISGGTNIYPREVEEALLTHPAVREVSAIGVADPEWGETVVACVVLADGSEPSDTALDAHCLAAIARFKR
PKRYVYLEALPKNNYGKVLKTELRKMVT
>Mature_508_residues
MNLSEWLAASARLRPSAPALLTGTTIEADYATFAQRAASFAAALQRDYGIVSGDRVALFAHNCTQYLEALYGIWWAGAVA
VPINAKLHGKEAAWICSNSGAKLALICDDTADTFNEAAGELPARMATLALDSDAYIRARSGDGPAAPAAREDGDLAWLFY
TSGTTGRPKGVMLSHGNLIAMSLCYLADVDTVSSDDAALYAAPISHGAGLYNMIHTRFGARHVVPASKGFDPDEVLTLGK
QLGNVAMFAAPTMVKRLVEAARRRGERGEGLRTIVYGGGPMYLADIRDALDVMGQRFVQIYGQGESPMAITSLKRELHAD
VDHPRYLQRLASVGTAQSALSVRITGPDGEVLPAGETGEIEAKGPTVMLGYWNNSDANAETLKDGWLRTGDVGRLDEDGF
LTLSDRSKDVIISGGTNIYPREVEEALLTHPAVREVSAIGVADPEWGETVVACVVLADGSEPSDTALDAHCLAAIARFKR
PKRYVYLEALPKNNYGKVLKTELRKMVT

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=507, Percent_Identity=27.2189349112426, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI187761343, Length=507, Percent_Identity=27.2189349112426, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI156151445, Length=518, Percent_Identity=27.2200772200772, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI42544132, Length=540, Percent_Identity=25, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI157311624, Length=519, Percent_Identity=26.2042389210019, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157311622, Length=519, Percent_Identity=26.2042389210019, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=25.9047619047619, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI58082049, Length=519, Percent_Identity=26.3969171483622, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI38505220, Length=530, Percent_Identity=25.4716981132075, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=24.6628131021195, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI27477105, Length=332, Percent_Identity=29.2168674698795, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI28416953, Length=127, Percent_Identity=33.0708661417323, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=29.8279158699809, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=28.0834914611006, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI221142682, Length=477, Percent_Identity=28.7211740041929, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1786810, Length=504, Percent_Identity=24.0079365079365, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788595, Length=460, Percent_Identity=26.5217391304348, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786529, Length=550, Percent_Identity=24.7272727272727, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786801, Length=453, Percent_Identity=22.075055187638, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790505, Length=129, Percent_Identity=30.2325581395349, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=489, Percent_Identity=31.2883435582822, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=29.3885601577909, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71994690, Length=530, Percent_Identity=27.1698113207547, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=373, Percent_Identity=32.4396782841823, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=530, Percent_Identity=27.1698113207547, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=530, Percent_Identity=26.7924528301887, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560308, Length=533, Percent_Identity=26.8292682926829, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=477, Percent_Identity=24.7379454926625, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=515, Percent_Identity=24.4660194174757, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=24.4660194174757, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=359, Percent_Identity=25.6267409470752, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=477, Percent_Identity=23.8993710691824, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=359, Percent_Identity=26.4623955431755, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=502, Percent_Identity=25.6972111553785, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323182, Length=562, Percent_Identity=22.5978647686833, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=539, Percent_Identity=23.3766233766234, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581924, Length=515, Percent_Identity=27.7669902912621, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI18859661, Length=506, Percent_Identity=27.8656126482213, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI21355181, Length=496, Percent_Identity=25, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20130357, Length=495, Percent_Identity=25.0505050505051, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648260, Length=351, Percent_Identity=26.4957264957265, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19922652, Length=501, Percent_Identity=24.5508982035928, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648255, Length=523, Percent_Identity=24.4741873804971, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648253, Length=523, Percent_Identity=24.282982791587, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356947, Length=358, Percent_Identity=25.1396648044693, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=23.6947791164659, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648257, Length=487, Percent_Identity=25.0513347022587, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653035, Length=362, Percent_Identity=24.0331491712707, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356441, Length=473, Percent_Identity=22.6215644820296, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19921316, Length=484, Percent_Identity=25.2066115702479, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161076582, Length=370, Percent_Identity=23.5135135135135, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358303, Length=473, Percent_Identity=21.9873150105708, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281365686, Length=444, Percent_Identity=22.7477477477477, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 54405; Mature: 54405

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEWLAASARLRPSAPALLTGTTIEADYATFAQRAASFAAALQRDYGIVSGDRVALFA
CCHHHHHHHHCCCCCCCCEEEECCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
HNCTQYLEALYGIWWAGAVAVPINAKLHGKEAAWICSNSGAKLALICDDTADTFNEAAGE
HHHHHHHHHHHHHHHHCEEEEEECCEECCCCEEEEEECCCCEEEEEECCCHHHHHHHHHH
LPARMATLALDSDAYIRARSGDGPAAPAAREDGDLAWLFYTSGTTGRPKGVMLSHGNLIA
CCHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCHH
MSLCYLADVDTVSSDDAALYAAPISHGAGLYNMIHTRFGARHVVPASKGFDPDEVLTLGK
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHH
QLGNVAMFAAPTMVKRLVEAARRRGERGEGLRTIVYGGGPMYLADIRDALDVMGQRFVQI
HHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHEE
YGQGESPMAITSLKRELHADVDHPRYLQRLASVGTAQSALSVRITGPDGEVLPAGETGEI
ECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEECCCCCCEE
EAKGPTVMLGYWNNSDANAETLKDGWLRTGDVGRLDEDGFLTLSDRSKDVIISGGTNIYP
EECCCEEEEEEECCCCCCHHHHHCCCEECCCCCCCCCCCEEEECCCCCEEEEECCCCCCH
REVEEALLTHPAVREVSAIGVADPEWGETVVACVVLADGSEPSDTALDAHCLAAIARFKR
HHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
PKRYVYLEALPKNNYGKVLKTELRKMVT
CCEEEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNLSEWLAASARLRPSAPALLTGTTIEADYATFAQRAASFAAALQRDYGIVSGDRVALFA
CCHHHHHHHHCCCCCCCCEEEECCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
HNCTQYLEALYGIWWAGAVAVPINAKLHGKEAAWICSNSGAKLALICDDTADTFNEAAGE
HHHHHHHHHHHHHHHHCEEEEEECCEECCCCEEEEEECCCCEEEEEECCCHHHHHHHHHH
LPARMATLALDSDAYIRARSGDGPAAPAAREDGDLAWLFYTSGTTGRPKGVMLSHGNLIA
CCHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCHH
MSLCYLADVDTVSSDDAALYAAPISHGAGLYNMIHTRFGARHVVPASKGFDPDEVLTLGK
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHH
QLGNVAMFAAPTMVKRLVEAARRRGERGEGLRTIVYGGGPMYLADIRDALDVMGQRFVQI
HHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHEE
YGQGESPMAITSLKRELHADVDHPRYLQRLASVGTAQSALSVRITGPDGEVLPAGETGEI
ECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEECCCCCCEE
EAKGPTVMLGYWNNSDANAETLKDGWLRTGDVGRLDEDGFLTLSDRSKDVIISGGTNIYP
EECCCEEEEEEECCCCCCHHHHHCCCEECCCCCCCCCCCEEEECCCCCEEEEECCCCCCH
REVEEALLTHPAVREVSAIGVADPEWGETVVACVVLADGSEPSDTALDAHCLAAIARFKR
HHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
PKRYVYLEALPKNNYGKVLKTELRKMVT
CCEEEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA