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The map label for this gene is murF [H]

Identifier: 86749113

GI number: 86749113

Start: 2260451

End: 2261890

Strand: Direct

Name: murF [H]

Synonym: RPB_1990

Alternate gene names: 86749113

Gene position: 2260451-2261890 (Clockwise)

Preceding gene: 86749112

Following gene: 86749114

Centisome position: 42.4

GC content: 68.82

Gene sequence:

>1440_bases
ATGAGCAAACAACCGCTATGGACGTCCGATGCGATCATCGCGGCGATGGGCGCGGCGGTGCAGGGCACGCCGGCCCGCGA
CGTGTTCGGCATCTCGATCGACAGCCGCACGCTGGCGCCGGGCGACGCTTACTTCGCGATCAGGGGCGACGTTCACGACG
GCCATGATTTCGTCGAGGCGGCGCTGAAGGCCGGCGCCGCCCTGGCGGTGGTCGAGACCGCGCAGCGCGGCAAATTCGCC
CCCGATGCGCCGCTGCTGCTGGTCGACGACGTGCTCGACGGCCTGCGCGACTTGGGACGCGCGGCGCGGGCGCGGCTCGA
CGCGAAGGTGATCGCGGTGACCGGCTCGGTCGGCAAGACCTCGACCAAGGAGGCGCTGCGCGGCGTGCTCGGCGCGCAGG
GCGAGACCCACGCTTCGGTGGCGTCGTTCAACAATCATTGGGGCGTGCCGTTGACGCTGGCGCGCTGCCCGGCGAGCGTG
CGCTATGCGGTGTTCGAGATCGGGATGAACCACGCCGGCGAAATTGAACCGCTGGTCAAGATGGTGCGACCACATCTGGC
GATCATCACCACGGTGGAGCCGGTGCATCTCGAATTCTTCTCCGGCATCGAGGCGATCGCCGACGCCAAGGCGGAAATCT
TCTCAGGGCTGGAGCCCGGCGGCATTGCCGTGCTGAACCGCGACAATCCGATGTTCAAACGGCTCACCGCGAGCGCCCGC
AAAGCCGGAGTCGAACGCATCGTCTCGTTCGGCGTCCACGCCAAGGCCGATGCGCGGCTGCGCGACATTGCGCTGCATGC
GGATTGCTCAGCGGTGGATGCGAGCATCCTCGGGCACGACGTCACCTACAAGCTCAACATCCCCGGCCGCCACATCGCGA
TGAACTCGCTCGCCGTGCTGGCCGCGGCGGACCTGGTCGGCGCCGACCTGGCGCTGGCCGCGCTGGCACTGGCGCAGGTC
GAGCCCGCGGCCGGGCGCGGCGTTCGCCGCACCCTCGAACTCGGGCAGGGCGCGGCGACGCTGATCGACGAGAGCTACAA
CGCCAATCCGGCGTCGATGGCGGCGGCGCTGGGCGTGCTCGGCAGCGCCGAGATCAGCGGGCAGGGGCGGCGAATCGCGG
TGCTCGGCGACATGCTGGAACTCGGTCCGGCCGGGCCTGAGCTGCATCGCGGCCTCGCCGGAACGGTGCAGAGCAACGCG
GTCGATCTGGTGTTCTGCTGCGGCCCCCTGATGCACAATCTGTGGGATGCCCTTTCCTCGGGCAAACGAGGCGGCTATGC
AGAGACTGCGGCCGCGCTCGAATCGCAGGTGGTTGCGGCAGTCCGGTCCGGCGACGTGCTGATGATCAAAGGCTCGCTCG
GCTCGCGCATGAAAACGATTGTCACCGCGCTCGAGAAGCGCTTTCCCGGCAAGGCCGCGCTCGACGACGCTGCGGTGTGA

Upstream 100 bases:

>100_bases
TCGCCGGCAAGGGCCATGAAACCGGGCAGATCGTGGGCGACACGGTGCTGCCTTTCAGTGACCACGAAGCCGCCGCGGCG
GCGTTGTCAGCGAGCGCCGC

Downstream 100 bases:

>100_bases
GGCGGCTTCAATGCTCTATTGGCTGATCGATTTTTCCAGCTCGTTTCCTGCTTTCAACGTCTTCCGTTACATCACCTTCC
GCACCGGCGGCGCGGTGGTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSKQPLWTSDAIIAAMGAAVQGTPARDVFGISIDSRTLAPGDAYFAIRGDVHDGHDFVEAALKAGAALAVVETAQRGKFA
PDAPLLLVDDVLDGLRDLGRAARARLDAKVIAVTGSVGKTSTKEALRGVLGAQGETHASVASFNNHWGVPLTLARCPASV
RYAVFEIGMNHAGEIEPLVKMVRPHLAIITTVEPVHLEFFSGIEAIADAKAEIFSGLEPGGIAVLNRDNPMFKRLTASAR
KAGVERIVSFGVHAKADARLRDIALHADCSAVDASILGHDVTYKLNIPGRHIAMNSLAVLAAADLVGADLALAALALAQV
EPAAGRGVRRTLELGQGAATLIDESYNANPASMAAALGVLGSAEISGQGRRIAVLGDMLELGPAGPELHRGLAGTVQSNA
VDLVFCCGPLMHNLWDALSSGKRGGYAETAAALESQVVAAVRSGDVLMIKGSLGSRMKTIVTALEKRFPGKAALDDAAV

Sequences:

>Translated_479_residues
MSKQPLWTSDAIIAAMGAAVQGTPARDVFGISIDSRTLAPGDAYFAIRGDVHDGHDFVEAALKAGAALAVVETAQRGKFA
PDAPLLLVDDVLDGLRDLGRAARARLDAKVIAVTGSVGKTSTKEALRGVLGAQGETHASVASFNNHWGVPLTLARCPASV
RYAVFEIGMNHAGEIEPLVKMVRPHLAIITTVEPVHLEFFSGIEAIADAKAEIFSGLEPGGIAVLNRDNPMFKRLTASAR
KAGVERIVSFGVHAKADARLRDIALHADCSAVDASILGHDVTYKLNIPGRHIAMNSLAVLAAADLVGADLALAALALAQV
EPAAGRGVRRTLELGQGAATLIDESYNANPASMAAALGVLGSAEISGQGRRIAVLGDMLELGPAGPELHRGLAGTVQSNA
VDLVFCCGPLMHNLWDALSSGKRGGYAETAAALESQVVAAVRSGDVLMIKGSLGSRMKTIVTALEKRFPGKAALDDAAV
>Mature_478_residues
SKQPLWTSDAIIAAMGAAVQGTPARDVFGISIDSRTLAPGDAYFAIRGDVHDGHDFVEAALKAGAALAVVETAQRGKFAP
DAPLLLVDDVLDGLRDLGRAARARLDAKVIAVTGSVGKTSTKEALRGVLGAQGETHASVASFNNHWGVPLTLARCPASVR
YAVFEIGMNHAGEIEPLVKMVRPHLAIITTVEPVHLEFFSGIEAIADAKAEIFSGLEPGGIAVLNRDNPMFKRLTASARK
AGVERIVSFGVHAKADARLRDIALHADCSAVDASILGHDVTYKLNIPGRHIAMNSLAVLAAADLVGADLALAALALAQVE
PAAGRGVRRTLELGQGAATLIDESYNANPASMAAALGVLGSAEISGQGRRIAVLGDMLELGPAGPELHRGLAGTVQSNAV
DLVFCCGPLMHNLWDALSSGKRGGYAETAAALESQVVAAVRSGDVLMIKGSLGSRMKTIVTALEKRFPGKAALDDAAV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=449, Percent_Identity=32.739420935412, Blast_Score=182, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49618; Mature: 49486

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQPLWTSDAIIAAMGAAVQGTPARDVFGISIDSRTLAPGDAYFAIRGDVHDGHDFVEA
CCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHH
ALKAGAALAVVETAQRGKFAPDAPLLLVDDVLDGLRDLGRAARARLDAKVIAVTGSVGKT
HHHCCCEEEEEHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
STKEALRGVLGAQGETHASVASFNNHWGVPLTLARCPASVRYAVFEIGMNHAGEIEPLVK
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEHHHCCCHHHHHHEEECCCCCCCHHHHHH
MVRPHLAIITTVEPVHLEFFSGIEAIADAKAEIFSGLEPGGIAVLNRDNPMFKRLTASAR
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
KAGVERIVSFGVHAKADARLRDIALHADCSAVDASILGHDVTYKLNIPGRHIAMNSLAVL
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCCEEHHHHHHHH
AAADLVGADLALAALALAQVEPAAGRGVRRTLELGQGAATLIDESYNANPASMAAALGVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
GSAEISGQGRRIAVLGDMLELGPAGPELHRGLAGTVQSNAVDLVFCCGPLMHNLWDALSS
CCCEECCCCCEEEEEHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHC
GKRGGYAETAAALESQVVAAVRSGDVLMIKGSLGSRMKTIVTALEKRFPGKAALDDAAV
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SKQPLWTSDAIIAAMGAAVQGTPARDVFGISIDSRTLAPGDAYFAIRGDVHDGHDFVEA
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHH
ALKAGAALAVVETAQRGKFAPDAPLLLVDDVLDGLRDLGRAARARLDAKVIAVTGSVGKT
HHHCCCEEEEEHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
STKEALRGVLGAQGETHASVASFNNHWGVPLTLARCPASVRYAVFEIGMNHAGEIEPLVK
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEHHHCCCHHHHHHEEECCCCCCCHHHHHH
MVRPHLAIITTVEPVHLEFFSGIEAIADAKAEIFSGLEPGGIAVLNRDNPMFKRLTASAR
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
KAGVERIVSFGVHAKADARLRDIALHADCSAVDASILGHDVTYKLNIPGRHIAMNSLAVL
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCCEEHHHHHHHH
AAADLVGADLALAALALAQVEPAAGRGVRRTLELGQGAATLIDESYNANPASMAAALGVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
GSAEISGQGRRIAVLGDMLELGPAGPELHRGLAGTVQSNAVDLVFCCGPLMHNLWDALSS
CCCEECCCCCEEEEEHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHC
GKRGGYAETAAALESQVVAAVRSGDVLMIKGSLGSRMKTIVTALEKRFPGKAALDDAAV
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]