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The map label for this gene is murE [H]

Identifier: 86749112

GI number: 86749112

Start: 2258997

End: 2260454

Strand: Direct

Name: murE [H]

Synonym: RPB_1989

Alternate gene names: 86749112

Gene position: 2258997-2260454 (Clockwise)

Preceding gene: 86749111

Following gene: 86749113

Centisome position: 42.37

GC content: 70.58

Gene sequence:

>1458_bases
ATGAAGCTCCGCGACCTCTTCAGCGACGACACGGCGATCACGGTGCAGGCGGCCGCTCTCGATGTCGGCGGCCTCGCGGT
CGACAGCCGAGCGGTGAAGCCGGGCGATGTGTTCTTTGCGCTGGCCGGGGCGAAGACCGACGGGGCGCGCTTCGTCGCCG
CTGCGATCGAGGCAGGCGCCGTCGCGGTGGCTGGCGATCATCCGCCCGCGGGCGACCTCGCCGTGCCCTTCGTTCGCCTG
ACCAATCCGCGCCGCGCGCTGGCGCTCGCCGCCGCGCGGATGTTTCCGCAGCAGCCGCAGACCATCGCGGCGGTCACCGG
CACCAGCGGCAAGACTTCGGTGGCAGCGTTTACGAGGCAGATCTGGCAGCGGCTGGGTCATGCTTCGGCCAGCATCGGCA
CCATCGGTCTCGTGACGCCGACGCGCAGCGTCTACGGCTCGCTGACCACGCCGGATCCGATCGCGCTGCATCGCTCGCTC
GACGCCATTGCGCGCGAGGGCGTCACCCATCTGGCGCTGGAAGCTTCGTCGCACGGGCTCGACCAGTTCCGCCTCGACGG
CGTCCGCGTCGCGGCGGGGGGATTCACCAATCTGTCGCGCGACCATATGGACTATCATCCCGACGTTGCGCACTACCTGA
ACGCCAAGCTGCGGCTGTTCCGCGATCTCGTGGTCGACGGCGGCGCCGCGGTGATCTCGGCCGATCACGACTGTTCGGCG
GAAGTGATCGATGCGGCTCGGGCGCGCGGGTTGCGGCTGATCAGCGTCGGCCGCAACGGCGACGCCGGCGCGGGCATCCG
GCTGATCGCGGCCGCGGTCGACGGCTTCGCGCAGCGGCTCACGCTCGACCATGCCGGGCGTAACTATCAAGTCCACCTGC
CGCTGGTCGGCGAATTCCAGATCGAGAATGCGCTGGTCGCGGCCGGGCTGGTGATCGGCACCGGCGGCGATCCGGCGGCG
GTGTTTGCCGCGCTCGATCATCTCGAGGGCGCGCCCGGCCGGCTGGAGCTGGTCGGCGAACGCAACGGCGCGCCGGTGTT
CGTCGACTATGCGCACAAGCCAGATGCTCTGGCCAAGTCGCTGCAGGCGCTGCGGCCTTATGCCAAGCGCAAGCTCGTTG
TGGTGTTCGGCGCCGGCGGCGATCGCGACGCCGGCAAGCGGCCGCTGATGGGCGCGATCGCGGCCGAGAGCGCCGACGCC
GTGATCGTCACCGACGACAATCCGCGCCGCGAAGACCCCGCCACGATCCGCGCCGCGATTCTCGCAGCAGCGCCGGGCGC
GCGCGAGATCGGCGACCGGGCCGAGGCGATCCGAACAGCGATCGGCGAATTGCAGCCGGGCGACGCGCTGGTGATCGCCG
GCAAGGGCCATGAAACCGGGCAGATCGTGGGCGACACGGTGCTGCCTTTCAGTGACCACGAAGCCGCCGCGGCGGCGTTG
TCAGCGAGCGCCGCATGA

Upstream 100 bases:

>100_bases
GGCGGCAATGCGTCAGTCGTGTTGCACCGGGGCGCGCTTGCGCTAAAAGCCCGGTCTGCGTGCGAACTCCCTTCGGACCG
ACCGACCGGACTCGAGGACC

Downstream 100 bases:

>100_bases
GCAAACAACCGCTATGGACGTCCGATGCGATCATCGCGGCGATGGGCGCGGCGGTGCAGGGCACGCCGGCCCGCGACGTG
TTCGGCATCTCGATCGACAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKLRDLFSDDTAITVQAAALDVGGLAVDSRAVKPGDVFFALAGAKTDGARFVAAAIEAGAVAVAGDHPPAGDLAVPFVRL
TNPRRALALAAARMFPQQPQTIAAVTGTSGKTSVAAFTRQIWQRLGHASASIGTIGLVTPTRSVYGSLTTPDPIALHRSL
DAIAREGVTHLALEASSHGLDQFRLDGVRVAAGGFTNLSRDHMDYHPDVAHYLNAKLRLFRDLVVDGGAAVISADHDCSA
EVIDAARARGLRLISVGRNGDAGAGIRLIAAAVDGFAQRLTLDHAGRNYQVHLPLVGEFQIENALVAAGLVIGTGGDPAA
VFAALDHLEGAPGRLELVGERNGAPVFVDYAHKPDALAKSLQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAAESADA
VIVTDDNPRREDPATIRAAILAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHETGQIVGDTVLPFSDHEAAAAAL
SASAA

Sequences:

>Translated_485_residues
MKLRDLFSDDTAITVQAAALDVGGLAVDSRAVKPGDVFFALAGAKTDGARFVAAAIEAGAVAVAGDHPPAGDLAVPFVRL
TNPRRALALAAARMFPQQPQTIAAVTGTSGKTSVAAFTRQIWQRLGHASASIGTIGLVTPTRSVYGSLTTPDPIALHRSL
DAIAREGVTHLALEASSHGLDQFRLDGVRVAAGGFTNLSRDHMDYHPDVAHYLNAKLRLFRDLVVDGGAAVISADHDCSA
EVIDAARARGLRLISVGRNGDAGAGIRLIAAAVDGFAQRLTLDHAGRNYQVHLPLVGEFQIENALVAAGLVIGTGGDPAA
VFAALDHLEGAPGRLELVGERNGAPVFVDYAHKPDALAKSLQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAAESADA
VIVTDDNPRREDPATIRAAILAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHETGQIVGDTVLPFSDHEAAAAAL
SASAA
>Mature_485_residues
MKLRDLFSDDTAITVQAAALDVGGLAVDSRAVKPGDVFFALAGAKTDGARFVAAAIEAGAVAVAGDHPPAGDLAVPFVRL
TNPRRALALAAARMFPQQPQTIAAVTGTSGKTSVAAFTRQIWQRLGHASASIGTIGLVTPTRSVYGSLTTPDPIALHRSL
DAIAREGVTHLALEASSHGLDQFRLDGVRVAAGGFTNLSRDHMDYHPDVAHYLNAKLRLFRDLVVDGGAAVISADHDCSA
EVIDAARARGLRLISVGRNGDAGAGIRLIAAAVDGFAQRLTLDHAGRNYQVHLPLVGEFQIENALVAAGLVIGTGGDPAA
VFAALDHLEGAPGRLELVGERNGAPVFVDYAHKPDALAKSLQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAAESADA
VIVTDDNPRREDPATIRAAILAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHETGQIVGDTVLPFSDHEAAAAAL
SASAA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=481, Percent_Identity=37.2141372141372, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 49995; Mature: 49995

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRDLFSDDTAITVQAAALDVGGLAVDSRAVKPGDVFFALAGAKTDGARFVAAAIEAGA
CCCHHHCCCCCEEEEEEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCEEEEEECCCCE
VAVAGDHPPAGDLAVPFVRLTNPRRALALAAARMFPQQPQTIAAVTGTSGKTSVAAFTRQ
EEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
IWQRLGHASASIGTIGLVTPTRSVYGSLTTPDPIALHRSLDAIAREGVTHLALEASSHGL
HHHHHCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
DQFRLDGVRVAAGGFTNLSRDHMDYHPDVAHYLNAKLRLFRDLVVDGGAAVISADHDCSA
CCEEECCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
EVIDAARARGLRLISVGRNGDAGAGIRLIAAAVDGFAQRLTLDHAGRNYQVHLPLVGEFQ
HHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECEE
IENALVAAGLVIGTGGDPAAVFAALDHLEGAPGRLELVGERNGAPVFVDYAHKPDALAKS
ECCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHH
LQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAAESADAVIVTDDNPRREDPATIRAAI
HHHHCHHHCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
LAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHETGQIVGDTVLPFSDHEAAAAAL
HHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECCEECCCCCCHHHHHHH
SASAA
HCCCC
>Mature Secondary Structure
MKLRDLFSDDTAITVQAAALDVGGLAVDSRAVKPGDVFFALAGAKTDGARFVAAAIEAGA
CCCHHHCCCCCEEEEEEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCEEEEEECCCCE
VAVAGDHPPAGDLAVPFVRLTNPRRALALAAARMFPQQPQTIAAVTGTSGKTSVAAFTRQ
EEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
IWQRLGHASASIGTIGLVTPTRSVYGSLTTPDPIALHRSLDAIAREGVTHLALEASSHGL
HHHHHCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
DQFRLDGVRVAAGGFTNLSRDHMDYHPDVAHYLNAKLRLFRDLVVDGGAAVISADHDCSA
CCEEECCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
EVIDAARARGLRLISVGRNGDAGAGIRLIAAAVDGFAQRLTLDHAGRNYQVHLPLVGEFQ
HHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECEE
IENALVAAGLVIGTGGDPAAVFAALDHLEGAPGRLELVGERNGAPVFVDYAHKPDALAKS
ECCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHH
LQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAAESADAVIVTDDNPRREDPATIRAAI
HHHHCHHHCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
LAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHETGQIVGDTVLPFSDHEAAAAAL
HHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECCEECCCCCCHHHHHHH
SASAA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]