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The map label for this gene is alkB [H]

Identifier: 86748824

GI number: 86748824

Start: 1935485

End: 1936156

Strand: Direct

Name: alkB [H]

Synonym: RPB_1701

Alternate gene names: 86748824

Gene position: 1935485-1936156 (Clockwise)

Preceding gene: 86748823

Following gene: 86748825

Centisome position: 36.3

GC content: 67.71

Gene sequence:

>672_bases
GTGAGGAAAGGGAGTGCGCCTTTGGCGGCCGATCTATTCGAGAATCTGTTCGATGGCCCGAGGCGCGAGGACATCGCTCC
GGGCGCAGCGCTGCTGCACGGCTTCGCGCGGGCGCAGGAGCTCGAATTGATGGCGGTGATCGAGGCCGTCGTCGCGCGCG
CCCCGTTCCGCCACATGATGACGCCGGGTGGCCATACGATGAGCGTGGCGATGACCAGTTGCGGCCGTGCCGGCTGGGTC
ACCGATCGTCGCGGATATCGCTATGCCACCCACGATCCGGCGAGCGAGCTGCCATGGCCGGAGATGCCGGACGTGTTGCG
CGAACTGGCGGTCAGCGCGGCGGCGGAAGCGGGGTTCGCGGCCTTCACGCCCGATGCCTGCCTGATCAATCGCTATGCGC
CGGGTGCCAAGATGGCGCTGCATCAGGACAAGGACGAGCAGGATTTCGCCGCCCCGATCGTCTCGGTGTCGCTCGGCCTG
CCGGCGGTCTTCCAATTCGGCGGGATGGCGCGCAGCGACAAGCCTCGCCGTTTCGAATTGCGCCACGGCGACGTGCTGGT
GTGGGGCGGCGAGACCCGGCTGGTGTATCACGGCGTGCTGGCGCTGAAGGACGGCGAGCACCCGCTGCTCGGCCGACAGC
GCATCAACCTGACGTTTCGCAAGGCGCTGTAA

Upstream 100 bases:

>100_bases
ATCGTGTATCCGACATCGCGATTATTGTCGGCCAAGGTCCGCACGTTCGTCGATCTCGCGGCGGAGATTTGCGACTGGCG
GTTCGGCGCGGGATAGCATC

Downstream 100 bases:

>100_bases
GTGCGGCAGATCACTTTGACCGCACCGCAATCTCCTCTATCCTGACGGCGGGAGAGGGCTGATGACCGAGCTTGCAATCG
ACGATGACAATTCCGCCAGC

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRKGSAPLAADLFENLFDGPRREDIAPGAALLHGFARAQELELMAVIEAVVARAPFRHMMTPGGHTMSVAMTSCGRAGWV
TDRRGYRYATHDPASELPWPEMPDVLRELAVSAAAEAGFAAFTPDACLINRYAPGAKMALHQDKDEQDFAAPIVSVSLGL
PAVFQFGGMARSDKPRRFELRHGDVLVWGGETRLVYHGVLALKDGEHPLLGRQRINLTFRKAL

Sequences:

>Translated_223_residues
MRKGSAPLAADLFENLFDGPRREDIAPGAALLHGFARAQELELMAVIEAVVARAPFRHMMTPGGHTMSVAMTSCGRAGWV
TDRRGYRYATHDPASELPWPEMPDVLRELAVSAAAEAGFAAFTPDACLINRYAPGAKMALHQDKDEQDFAAPIVSVSLGL
PAVFQFGGMARSDKPRRFELRHGDVLVWGGETRLVYHGVLALKDGEHPLLGRQRINLTFRKAL
>Mature_223_residues
MRKGSAPLAADLFENLFDGPRREDIAPGAALLHGFARAQELELMAVIEAVVARAPFRHMMTPGGHTMSVAMTSCGRAGWV
TDRRGYRYATHDPASELPWPEMPDVLRELAVSAAAEAGFAAFTPDACLINRYAPGAKMALHQDKDEQDFAAPIVSVSLGL
PAVFQFGGMARSDKPRRFELRHGDVLVWGGETRLVYHGVLALKDGEHPLLGRQRINLTFRKAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=121, Percent_Identity=30.5785123966942, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1788541, Length=203, Percent_Identity=58.128078817734, Blast_Score=245, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45555401, Length=121, Percent_Identity=34.7107438016529, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24388; Mature: 24388

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGSAPLAADLFENLFDGPRREDIAPGAALLHGFARAQELELMAVIEAVVARAPFRHMM
CCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
TPGGHTMSVAMTSCGRAGWVTDRRGYRYATHDPASELPWPEMPDVLRELAVSAAAEAGFA
CCCCCEEEEEEHHCCCCCCCCCCCCCEECCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCE
AFTPDACLINRYAPGAKMALHQDKDEQDFAAPIVSVSLGLPAVFQFGGMARSDKPRRFEL
EECCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEE
RHGDVLVWGGETRLVYHGVLALKDGEHPLLGRQRINLTFRKAL
CCCCEEEECCCEEEEEEEEEEEECCCCCCCCCEEECEEEECCC
>Mature Secondary Structure
MRKGSAPLAADLFENLFDGPRREDIAPGAALLHGFARAQELELMAVIEAVVARAPFRHMM
CCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
TPGGHTMSVAMTSCGRAGWVTDRRGYRYATHDPASELPWPEMPDVLRELAVSAAAEAGFA
CCCCCEEEEEEHHCCCCCCCCCCCCCEECCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCE
AFTPDACLINRYAPGAKMALHQDKDEQDFAAPIVSVSLGLPAVFQFGGMARSDKPRRFEL
EECCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEE
RHGDVLVWGGETRLVYHGVLALKDGEHPLLGRQRINLTFRKAL
CCCCEEEECCCEEEEEEEEEEEECCCCCCCCCEEECEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855 [H]