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The map label for this gene is lcfB [H]

Identifier: 86748809

GI number: 86748809

Start: 1919334

End: 1921208

Strand: Direct

Name: lcfB [H]

Synonym: RPB_1686

Alternate gene names: 86748809

Gene position: 1919334-1921208 (Clockwise)

Preceding gene: 86748808

Following gene: 86748810

Centisome position: 36.0

GC content: 66.61

Gene sequence:

>1875_bases
ATGACTTCAAGCGCCGCCGCCGTGATGACCAAGCCGGCCTTCCGCAAGGTCAACTGGCTGCCCCGCGAGATCGAGGTCGA
GCGCCGTGCCGACGGCGTCGTGGTGATGAAATCGCGCTTTCCGCTGATGGACCACGATCCGCATCTGCCAATGGCGCTGG
CGAAATGGGCCAAGCAGCGCCCCGACGACATCTGGCTGGCGCAGCGCAAGGGCGAGGCGCGGCAGTGGCACAAGGTCAGC
TACGCCGAGGCCAAGCGCATCGTTGATTCGCTCACCCAGGCGCTGCTCGACCTGAGGCTGCCGGAAGGCCGGCCGCTCAC
CGTGCTGTCCGGCAACTCGATCGAATTCGCGCTGATGATGATGGCGGCGATGCAGGCGCGGATTCCGACCGCGCCGGTGT
CGCCGGCCTATTCGCTGATGAGCCACGACCACGTCAAGCTGAAGTACCTGTTCGACCTGGTGAAGCCGTCGGTGGTGATG
GTGCAGAACGGTGCGCCGTTCACCAAGGCGCTGCACGCGCTGGATCTGACCGGCGTGTCCGTGATTCATGTCGACGGGAC
GGCCGACGGCATCCCCAGCCTGTCGTTCGCCGACATGGCGGCCACCAAGGTGACCGCCGACGTCGAGGCCTCGATCGCGA
AGATCACGCCCGACACCGTCGCCAAGTTCCTGTTCACCTCCGGCTCGACCGGGATGCCGAAGGCGGTGATCAATACCCAG
CGCATGATGTGCGCCAACGCGGCGATGATGATGCAGGTGCGGCCGCGCGATCCGAACGGTCCGCCGCCGGTGCAGCTCGA
CTGGATGCCGTGGAATCACACCATGGGGGGCAACGCCGCGTTCAATCCGCTGCTGATCGAAGGCGGCACGCTGTACATCG
ACGACGGCCGCCCGGTGCCCGGCCAGATCGAAGAGACGCTGCGCAACTTGCGCGAAATCTCGCCGACCTACTACGCCAAC
GTACCGGCCGGCTACGCCGCGCTGGCGAGCGCGATGGAGAAGGACGACGCGCTGTGCCGGTCGTTCTTCAAGGACATGCA
GCTGATGGCCTATGGCGGCGCCCGGCTGCCCGACGATCTGTACGAACGGATGCAGGCGCTGGCGGTGCGCGCCACCGGGC
ATCGGCTGGTGTTCTACACCGGCTGGGGCTCGACCGAGACTTCGCCGACTTCGACCGGCACTTATTGGGACACCGAGCGC
GTCGGCCTGATCGGGCTGCCGTTCCCCGGCGTCGAACTGAAGATGCTGCCGGTCGGCGACAAATACGAATTGCGGCTGCG
CGGCGTCAACGTGATGCCGGGCTATCACGGCCAGCCGGATCTCACCGAGAAGGCGTTCGACGAAGAGGGCTTCTACAAGA
TCGGCGATGCCGGCGTGTTCGTCGATCCGAACGATCCGGTGAAGGGTATCATCTTCGCCGGCCGGGTCGCCGAGGACTTC
AAGCTGATGACCGGCACCTTCGTCCACGTCAGCACGGTGCGCACCGACGCGATCGCCGCGGCGAGCCCGGTGATCCAGGA
CGCGCTGGTGGCGGGGCAGGACCGCGCTTATATCGGCCTGCTGGCGTGGCCCAACCTCAATGCCTGCCGGGTGATGATCG
GCGATCCGGGCGCGACCTTCGAGGACGTGGTCAGGCATCCGGCGGTGCTCGCCGCGCTGAAAGCCGGCCTGGAGAAGCAA
AACCGCGAGGTTGAAGGCGCCAGCTCGATGCGGATCGCGCGCGCGATGCTGATGGTCGAGCCGCCGTCGATCGACGGCAA
CGAACTCACCGACAAGGGCTACATCAACCAGCGCGCAGGCCTCGAGCGCCGCGCCGCGCTGGTCGAGCGGCTGTACGCCG
AGCATCCCGACCCGGACGTGATGGTGCTGCAGTGA

Upstream 100 bases:

>100_bases
CAGCGCCTTTCTGCAGAAGCGCAAGCCGGTTTATCGTCGACGCTAGCGCGGCAAGAACATCACGGCCCGGGAAGAGGCCA
GGAAAACGGGAGAATCAACG

Downstream 100 bases:

>100_bases
ATCAACTGACGGCCCAATCTGCACAGCGCAACCTCTCCCCGCCCTGCGCGGGGAGAGGTCGACCGGCGCATCGCGCCGGT
CGGGTGAGGGGCCTGGCACG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MTSSAAAVMTKPAFRKVNWLPREIEVERRADGVVVMKSRFPLMDHDPHLPMALAKWAKQRPDDIWLAQRKGEARQWHKVS
YAEAKRIVDSLTQALLDLRLPEGRPLTVLSGNSIEFALMMMAAMQARIPTAPVSPAYSLMSHDHVKLKYLFDLVKPSVVM
VQNGAPFTKALHALDLTGVSVIHVDGTADGIPSLSFADMAATKVTADVEASIAKITPDTVAKFLFTSGSTGMPKAVINTQ
RMMCANAAMMMQVRPRDPNGPPPVQLDWMPWNHTMGGNAAFNPLLIEGGTLYIDDGRPVPGQIEETLRNLREISPTYYAN
VPAGYAALASAMEKDDALCRSFFKDMQLMAYGGARLPDDLYERMQALAVRATGHRLVFYTGWGSTETSPTSTGTYWDTER
VGLIGLPFPGVELKMLPVGDKYELRLRGVNVMPGYHGQPDLTEKAFDEEGFYKIGDAGVFVDPNDPVKGIIFAGRVAEDF
KLMTGTFVHVSTVRTDAIAAASPVIQDALVAGQDRAYIGLLAWPNLNACRVMIGDPGATFEDVVRHPAVLAALKAGLEKQ
NREVEGASSMRIARAMLMVEPPSIDGNELTDKGYINQRAGLERRAALVERLYAEHPDPDVMVLQ

Sequences:

>Translated_624_residues
MTSSAAAVMTKPAFRKVNWLPREIEVERRADGVVVMKSRFPLMDHDPHLPMALAKWAKQRPDDIWLAQRKGEARQWHKVS
YAEAKRIVDSLTQALLDLRLPEGRPLTVLSGNSIEFALMMMAAMQARIPTAPVSPAYSLMSHDHVKLKYLFDLVKPSVVM
VQNGAPFTKALHALDLTGVSVIHVDGTADGIPSLSFADMAATKVTADVEASIAKITPDTVAKFLFTSGSTGMPKAVINTQ
RMMCANAAMMMQVRPRDPNGPPPVQLDWMPWNHTMGGNAAFNPLLIEGGTLYIDDGRPVPGQIEETLRNLREISPTYYAN
VPAGYAALASAMEKDDALCRSFFKDMQLMAYGGARLPDDLYERMQALAVRATGHRLVFYTGWGSTETSPTSTGTYWDTER
VGLIGLPFPGVELKMLPVGDKYELRLRGVNVMPGYHGQPDLTEKAFDEEGFYKIGDAGVFVDPNDPVKGIIFAGRVAEDF
KLMTGTFVHVSTVRTDAIAAASPVIQDALVAGQDRAYIGLLAWPNLNACRVMIGDPGATFEDVVRHPAVLAALKAGLEKQ
NREVEGASSMRIARAMLMVEPPSIDGNELTDKGYINQRAGLERRAALVERLYAEHPDPDVMVLQ
>Mature_623_residues
TSSAAAVMTKPAFRKVNWLPREIEVERRADGVVVMKSRFPLMDHDPHLPMALAKWAKQRPDDIWLAQRKGEARQWHKVSY
AEAKRIVDSLTQALLDLRLPEGRPLTVLSGNSIEFALMMMAAMQARIPTAPVSPAYSLMSHDHVKLKYLFDLVKPSVVMV
QNGAPFTKALHALDLTGVSVIHVDGTADGIPSLSFADMAATKVTADVEASIAKITPDTVAKFLFTSGSTGMPKAVINTQR
MMCANAAMMMQVRPRDPNGPPPVQLDWMPWNHTMGGNAAFNPLLIEGGTLYIDDGRPVPGQIEETLRNLREISPTYYANV
PAGYAALASAMEKDDALCRSFFKDMQLMAYGGARLPDDLYERMQALAVRATGHRLVFYTGWGSTETSPTSTGTYWDTERV
GLIGLPFPGVELKMLPVGDKYELRLRGVNVMPGYHGQPDLTEKAFDEEGFYKIGDAGVFVDPNDPVKGIIFAGRVAEDFK
LMTGTFVHVSTVRTDAIAAASPVIQDALVAGQDRAYIGLLAWPNLNACRVMIGDPGATFEDVVRHPAVLAALKAGLEKQN
REVEGASSMRIARAMLMVEPPSIDGNELTDKGYINQRAGLERRAALVERLYAEHPDPDVMVLQ

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=284, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693145, Length=486, Percent_Identity=22.8395061728395, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17564090, Length=299, Percent_Identity=28.0936454849498, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 68364; Mature: 68232

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSAAAVMTKPAFRKVNWLPREIEVERRADGVVVMKSRFPLMDHDPHLPMALAKWAKQR
CCCCCCHHHCCCHHHHCCCCCCCEEHEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCC
PDDIWLAQRKGEARQWHKVSYAEAKRIVDSLTQALLDLRLPEGRPLTVLSGNSIEFALMM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
MAAMQARIPTAPVSPAYSLMSHDHVKLKYLFDLVKPSVVMVQNGAPFTKALHALDLTGVS
HHHHHHCCCCCCCCHHHHHHCCCCEEHHHHHHHHCCCEEEEECCCCHHHHHHHHEECCEE
VIHVDGTADGIPSLSFADMAATKVTADVEASIAKITPDTVAKFLFTSGSTGMPKAVINTQ
EEEECCCCCCCCCCCHHHHHHHHEECCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHH
RMMCANAAMMMQVRPRDPNGPPPVQLDWMPWNHTMGGNAAFNPLLIEGGTLYIDDGRPVP
HHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCEEEEECCCCCC
GQIEETLRNLREISPTYYANVPAGYAALASAMEKDDALCRSFFKDMQLMAYGGARLPDDL
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHH
YERMQALAVRATGHRLVFYTGWGSTETSPTSTGTYWDTERVGLIGLPFPGVELKMLPVGD
HHHHHHHEEEECCCEEEEEECCCCCCCCCCCCCCEECCCCEEEEECCCCCCEEEEEECCC
KYELRLRGVNVMPGYHGQPDLTEKAFDEEGFYKIGDAGVFVDPNDPVKGIIFAGRVAEDF
CEEEEEEEEEECCCCCCCCCCHHHHCCCCCCEEECCCEEEECCCCCCCEEEEECCCCCCC
KLMTGTFVHVSTVRTDAIAAASPVIQDALVAGQDRAYIGLLAWPNLNACRVMIGDPGATF
EEEECEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCH
EDVVRHPAVLAALKAGLEKQNREVEGASSMRIARAMLMVEPPSIDGNELTDKGYINQRAG
HHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHCC
LERRAALVERLYAEHPDPDVMVLQ
HHHHHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure 
TSSAAAVMTKPAFRKVNWLPREIEVERRADGVVVMKSRFPLMDHDPHLPMALAKWAKQR
CCCCCHHHCCCHHHHCCCCCCCEEHEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCC
PDDIWLAQRKGEARQWHKVSYAEAKRIVDSLTQALLDLRLPEGRPLTVLSGNSIEFALMM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
MAAMQARIPTAPVSPAYSLMSHDHVKLKYLFDLVKPSVVMVQNGAPFTKALHALDLTGVS
HHHHHHCCCCCCCCHHHHHHCCCCEEHHHHHHHHCCCEEEEECCCCHHHHHHHHEECCEE
VIHVDGTADGIPSLSFADMAATKVTADVEASIAKITPDTVAKFLFTSGSTGMPKAVINTQ
EEEECCCCCCCCCCCHHHHHHHHEECCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHH
RMMCANAAMMMQVRPRDPNGPPPVQLDWMPWNHTMGGNAAFNPLLIEGGTLYIDDGRPVP
HHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCEEEEECCCCCC
GQIEETLRNLREISPTYYANVPAGYAALASAMEKDDALCRSFFKDMQLMAYGGARLPDDL
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHH
YERMQALAVRATGHRLVFYTGWGSTETSPTSTGTYWDTERVGLIGLPFPGVELKMLPVGD
HHHHHHHEEEECCCEEEEEECCCCCCCCCCCCCCEECCCCEEEEECCCCCCEEEEEECCC
KYELRLRGVNVMPGYHGQPDLTEKAFDEEGFYKIGDAGVFVDPNDPVKGIIFAGRVAEDF
CEEEEEEEEEECCCCCCCCCCHHHHCCCCCCEEECCCEEEECCCCCCCEEEEECCCCCCC
KLMTGTFVHVSTVRTDAIAAASPVIQDALVAGQDRAYIGLLAWPNLNACRVMIGDPGATF
EEEECEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCH
EDVVRHPAVLAALKAGLEKQNREVEGASSMRIARAMLMVEPPSIDGNELTDKGYINQRAG
HHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHCC
LERRAALVERLYAEHPDPDVMVLQ
HHHHHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]