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The map label for this gene is echA12 [H]

Identifier: 86748808

GI number: 86748808

Start: 1918473

End: 1919279

Strand: Direct

Name: echA12 [H]

Synonym: RPB_1685

Alternate gene names: 86748808

Gene position: 1918473-1919279 (Clockwise)

Preceding gene: 86748807

Following gene: 86748809

Centisome position: 35.98

GC content: 65.55

Gene sequence:

>807_bases
GTGCCCCAGGCGCTGCTGATCGAGCACACGGACGGCGTTGATTGGGTGACGCTCAATCGCCCGGACAGCCTGAACGCGCT
CAACCCCGAACTGATCGACGCATTGAACGTCTATTTCGGCCAGCTTCAGCGCAACCGCGACACGCGCGTGGTGGTGTTGA
AGGGCGCCGGCCGCGCGTTTTGCGCCGGGCTCGATCTCAAAGACGCGATGGAGCGCCGGGGCACCCAACAGGAGCCGCCG
GGGGTAACCGAATCTCTCGACTCGCAGCGCCGCATCGCCGACATCGTCATGCTGATGCGGCGTTGTCCGCAGCCGATCAT
CGCGCTGGTCCACGGCGCCGCTGCCGGTGGCGGCTTCGCGCTGGCGCTGGCGAGCGATATCCGCATCGCCGGCCGCAGCG
CGCGGATGAACTGCGCCTTCATCAAGCTCGGGCTCGGCGGCTGCGACATCGGCACCAGCTACTTCTTGCCGCGCCTGGTC
GGCGTCTCGGTCGCATCCGAACTGATTCTCACCGGCCGCTTCATCGAATCCGAGCGCGCGCTGATGACCGGACTGGTGTC
CGAACTGGTCGAGGACGACCAGCTCGCAACGGCGGCGCAGCCTTATGTCGACGCCATGCTGGCGGCGTCGCCGATCGGTC
TGCGGCTGTCGAAGGAATGCCTCAACATGGCGGTCGATGCCGGTTCGATCGAGCAGGTGATCGCGATGGAAGATCGCAAT
CAGGTGCTGTGCTCGCGTTCGGAGGATTTCGGCGAAGGCATCAGCGCCTTTCTGCAGAAGCGCAAGCCGGTTTATCGTCG
ACGCTAG

Upstream 100 bases:

>100_bases
CACGCGACGTCGACGCAAGGCATGCCCTCTCCCACAAGGGGAGAGGGCACAATAACGACCGCTGCGATCCGCGGACAAAA
CAAGATACGGGAGTGATTGC

Downstream 100 bases:

>100_bases
CGCGGCAAGAACATCACGGCCCGGGAAGAGGCCAGGAAAACGGGAGAATCAACGATGACTTCAAGCGCCGCCGCCGTGAT
GACCAAGCCGGCCTTCCGCA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPQALLIEHTDGVDWVTLNRPDSLNALNPELIDALNVYFGQLQRNRDTRVVVLKGAGRAFCAGLDLKDAMERRGTQQEPP
GVTESLDSQRRIADIVMLMRRCPQPIIALVHGAAAGGGFALALASDIRIAGRSARMNCAFIKLGLGGCDIGTSYFLPRLV
GVSVASELILTGRFIESERALMTGLVSELVEDDQLATAAQPYVDAMLAASPIGLRLSKECLNMAVDAGSIEQVIAMEDRN
QVLCSRSEDFGEGISAFLQKRKPVYRRR

Sequences:

>Translated_268_residues
MPQALLIEHTDGVDWVTLNRPDSLNALNPELIDALNVYFGQLQRNRDTRVVVLKGAGRAFCAGLDLKDAMERRGTQQEPP
GVTESLDSQRRIADIVMLMRRCPQPIIALVHGAAAGGGFALALASDIRIAGRSARMNCAFIKLGLGGCDIGTSYFLPRLV
GVSVASELILTGRFIESERALMTGLVSELVEDDQLATAAQPYVDAMLAASPIGLRLSKECLNMAVDAGSIEQVIAMEDRN
QVLCSRSEDFGEGISAFLQKRKPVYRRR
>Mature_267_residues
PQALLIEHTDGVDWVTLNRPDSLNALNPELIDALNVYFGQLQRNRDTRVVVLKGAGRAFCAGLDLKDAMERRGTQQEPPG
VTESLDSQRRIADIVMLMRRCPQPIIALVHGAAAGGGFALALASDIRIAGRSARMNCAFIKLGLGGCDIGTSYFLPRLVG
VSVASELILTGRFIESERALMTGLVSELVEDDQLATAAQPYVDAMLAASPIGLRLSKECLNMAVDAGSIEQVIAMEDRNQ
VLCSRSEDFGEGISAFLQKRKPVYRRR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=255, Percent_Identity=30.1960784313725, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=29.8449612403101, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI4502327, Length=272, Percent_Identity=31.9852941176471, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI31542718, Length=268, Percent_Identity=27.6119402985075, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=197, Percent_Identity=29.4416243654822, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI37594471, Length=197, Percent_Identity=29.4416243654822, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=223, Percent_Identity=26.457399103139, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260275230, Length=223, Percent_Identity=26.457399103139, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260274832, Length=223, Percent_Identity=26.457399103139, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=26.9767441860465, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=32.2404371584699, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136756, Length=217, Percent_Identity=24.4239631336406, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=217, Percent_Identity=24.4239631336406, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=217, Percent_Identity=24.4239631336406, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI68989263, Length=179, Percent_Identity=29.608938547486, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=37.0229007633588, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=32.1969696969697, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=31.9391634980989, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=27.4809160305344, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=29.1970802919708, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=33.160621761658, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=28.7958115183246, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=32.421875, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=270, Percent_Identity=29.6296296296296, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=29.3859649122807, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=32.1739130434783, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=29.6153846153846, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=29.2237442922374, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=27.8884462151394, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=30.3482587064677, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=221, Percent_Identity=30.316742081448, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=221, Percent_Identity=30.316742081448, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=187, Percent_Identity=27.807486631016, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653139, Length=276, Percent_Identity=32.2463768115942, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19920382, Length=272, Percent_Identity=32.3529411764706, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=33.6, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=33.6, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922422, Length=266, Percent_Identity=23.6842105263158, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=26.953125, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571729, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571730, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650670, Length=191, Percent_Identity=31.413612565445, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29101; Mature: 28970

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQALLIEHTDGVDWVTLNRPDSLNALNPELIDALNVYFGQLQRNRDTRVVVLKGAGRAF
CCCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
CAGLDLKDAMERRGTQQEPPGVTESLDSQRRIADIVMLMRRCPQPIIALVHGAAAGGGFA
EECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEE
LALASDIRIAGRSARMNCAFIKLGLGGCDIGTSYFLPRLVGVSVASELILTGRFIESERA
EEEEHHHEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMTGLVSELVEDDQLATAAQPYVDAMLAASPIGLRLSKECLNMAVDAGSIEQVIAMEDRN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC
QVLCSRSEDFGEGISAFLQKRKPVYRRR
CEECCCCCHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
PQALLIEHTDGVDWVTLNRPDSLNALNPELIDALNVYFGQLQRNRDTRVVVLKGAGRAF
CCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
CAGLDLKDAMERRGTQQEPPGVTESLDSQRRIADIVMLMRRCPQPIIALVHGAAAGGGFA
EECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEE
LALASDIRIAGRSARMNCAFIKLGLGGCDIGTSYFLPRLVGVSVASELILTGRFIESERA
EEEEHHHEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMTGLVSELVEDDQLATAAQPYVDAMLAASPIGLRLSKECLNMAVDAGSIEQVIAMEDRN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC
QVLCSRSEDFGEGISAFLQKRKPVYRRR
CEECCCCCHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]