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The map label for this gene is cysP [H]

Identifier: 86748523

GI number: 86748523

Start: 1589039

End: 1590049

Strand: Reverse

Name: cysP [H]

Synonym: RPB_1398

Alternate gene names: 86748523

Gene position: 1590049-1589039 (Counterclockwise)

Preceding gene: 86748529

Following gene: 86748522

Centisome position: 29.82

GC content: 63.4

Gene sequence:

>1011_bases
GTGAACCGCTTTGCTTTCGCCCTCGCCGCCGGCCTCGCCGCATTCGCGACCGCCATGCCGGCGCAAGCGCAGACGCCACC
CGCACCGCTGCTCAATGTCTCCTACGACATCGCGCGGGAACTCTATGCCGAGATCAACGCTGCATTCATCCCGCACTGGA
AGCAGACGACCGGCCAGGACATCAGCATCAACCAGTCGCATGGCGGCTCGTCGCGGCAGGCGCGCTCGATCCTCGAAGGG
CTCGAAGCCGACGTGGTGACCTTCAATCAGGTCACCGACGTCCAGGTGCTGCACGACAAGGGCAAGCTGATCCCGGCCGA
TTGGGCGAAGCGGCTGCCCAATAATTCATCGCCCTACTATTCGCTGCCGGCGTTCCTGGTGCGCGCCGGCAATCCGAAGG
GCATCAAGGATTGGGACGATCTGGTGAAGCCGGGCGTCAAGGTGATCTTCCCCAACCCGAAGACCTCCGGCAATGCACGC
TACACCTATCTCGCGGCCTACGCCTTCGCCAAGCATAAATACGGCAGCGAGGCCGAGGCCGACGCTTTTGTTAAAAAGCT
GTTCGCCAACGTGCCGGTGTTCGACACCGGCGGCCGCGCCGCGACCACCACCTTCGTCGAGCGCCAGACCGGCGACGTGC
TGATCACCTTCGAGGCCGAGACCAGCGCGATCCGCGACCTCGCCGGCGCCGACAAGTATCAAGTCGTGGTGCCGCCGACC
AGTGTGCTGGCCGAATTCCCCGTCAGCGTCGTCGACAAATACGCCGACAAGCACGGCACCCGCGCGCTCGCGACCGCCTA
TCTCGAATATCTGTATTCACCCGAGGGCCAGACCATCCTCGCCAAGGCCTATAACCGCGTCCACGACAAGGCCGTGATCG
CGCAATTCAAGGACAAGTTCCCCGAGGTCAAGCTGTATCGCGTCGAGGACGAATTCGGCGGCTGGGACAAGCTCAACGCC
GAACACCTCGCCTCCGGCGCGAAGCTCGATCAGCTGTTCGGAGGACGATAA

Upstream 100 bases:

>100_bases
TCGATGCTGCCTCGCCGAGGCGTCGAAGGCCGAGACCGCGCGCGAAAATCATCGCGCCCGGCTGCTGCTCGAGATGATAG
GCCAGCAATAAGGAGTTGTC

Downstream 100 bases:

>100_bases
GTCACAGGCACAGCGCAACCTCTCCCCGCGCGCGGGGAGAGGGCGACCGGCGGAGCGCAGCGAAGCCGGTCGGGTGAGGG
GGCGTCTCGACGCAGCCGAG

Product: thiosulphate-binding protein

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNRFAFALAAGLAAFATAMPAQAQTPPAPLLNVSYDIARELYAEINAAFIPHWKQTTGQDISINQSHGGSSRQARSILEG
LEADVVTFNQVTDVQVLHDKGKLIPADWAKRLPNNSSPYYSLPAFLVRAGNPKGIKDWDDLVKPGVKVIFPNPKTSGNAR
YTYLAAYAFAKHKYGSEAEADAFVKKLFANVPVFDTGGRAATTTFVERQTGDVLITFEAETSAIRDLAGADKYQVVVPPT
SVLAEFPVSVVDKYADKHGTRALATAYLEYLYSPEGQTILAKAYNRVHDKAVIAQFKDKFPEVKLYRVEDEFGGWDKLNA
EHLASGAKLDQLFGGR

Sequences:

>Translated_336_residues
MNRFAFALAAGLAAFATAMPAQAQTPPAPLLNVSYDIARELYAEINAAFIPHWKQTTGQDISINQSHGGSSRQARSILEG
LEADVVTFNQVTDVQVLHDKGKLIPADWAKRLPNNSSPYYSLPAFLVRAGNPKGIKDWDDLVKPGVKVIFPNPKTSGNAR
YTYLAAYAFAKHKYGSEAEADAFVKKLFANVPVFDTGGRAATTTFVERQTGDVLITFEAETSAIRDLAGADKYQVVVPPT
SVLAEFPVSVVDKYADKHGTRALATAYLEYLYSPEGQTILAKAYNRVHDKAVIAQFKDKFPEVKLYRVEDEFGGWDKLNA
EHLASGAKLDQLFGGR
>Mature_336_residues
MNRFAFALAAGLAAFATAMPAQAQTPPAPLLNVSYDIARELYAEINAAFIPHWKQTTGQDISINQSHGGSSRQARSILEG
LEADVVTFNQVTDVQVLHDKGKLIPADWAKRLPNNSSPYYSLPAFLVRAGNPKGIKDWDDLVKPGVKVIFPNPKTSGNAR
YTYLAAYAFAKHKYGSEAEADAFVKKLFANVPVFDTGGRAATTTFVERQTGDVLITFEAETSAIRDLAGADKYQVVVPPT
SVLAEFPVSVVDKYADKHGTRALATAYLEYLYSPEGQTILAKAYNRVHDKAVIAQFKDKFPEVKLYRVEDEFGGWDKLNA
EHLASGAKLDQLFGGR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=306, Percent_Identity=57.516339869281, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1790351, Length=333, Percent_Identity=50.7507507507508, Blast_Score=333, Evalue=9e-93,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36779; Mature: 36779

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFAFALAAGLAAFATAMPAQAQTPPAPLLNVSYDIARELYAEINAAFIPHWKQTTGQD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ISINQSHGGSSRQARSILEGLEADVVTFNQVTDVQVLHDKGKLIPADWAKRLPNNSSPYY
EEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHCCCCCCCCE
SLPAFLVRAGNPKGIKDWDDLVKPGVKVIFPNPKTSGNARYTYLAAYAFAKHKYGSEAEA
ECCHHEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCEEEHHHHHHHHHHCCCCCHHH
DAFVKKLFANVPVFDTGGRAATTTFVERQTGDVLITFEAETSAIRDLAGADKYQVVVPPT
HHHHHHHHHCCCEEECCCCEEEHHEEEECCCCEEEEEECCHHHHHHHCCCCCEEEEECCH
SVLAEFPVSVVDKYADKHGTRALATAYLEYLYSPEGQTILAKAYNRVHDKAVIAQFKDKF
HHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCC
PEVKLYRVEDEFGGWDKLNAEHLASGAKLDQLFGGR
CCEEEEEEECCCCCCCCCCHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MNRFAFALAAGLAAFATAMPAQAQTPPAPLLNVSYDIARELYAEINAAFIPHWKQTTGQD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ISINQSHGGSSRQARSILEGLEADVVTFNQVTDVQVLHDKGKLIPADWAKRLPNNSSPYY
EEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHCCCCCCCCE
SLPAFLVRAGNPKGIKDWDDLVKPGVKVIFPNPKTSGNARYTYLAAYAFAKHKYGSEAEA
ECCHHEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCEEEHHHHHHHHHHCCCCCHHH
DAFVKKLFANVPVFDTGGRAATTTFVERQTGDVLITFEAETSAIRDLAGADKYQVVVPPT
HHHHHHHHHCCCEEECCCCEEEHHEEEECCCCEEEEEECCHHHHHHHCCCCCEEEEECCH
SVLAEFPVSVVDKYADKHGTRALATAYLEYLYSPEGQTILAKAYNRVHDKAVIAQFKDKF
HHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCC
PEVKLYRVEDEFGGWDKLNAEHLASGAKLDQLFGGR
CCEEEEEEECCCCCCCCCCHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]