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The map label for this gene is fixK [H]

Identifier: 86748486

GI number: 86748486

Start: 1551956

End: 1552645

Strand: Reverse

Name: fixK [H]

Synonym: RPB_1361

Alternate gene names: 86748486

Gene position: 1552645-1551956 (Counterclockwise)

Preceding gene: 86748487

Following gene: 86748485

Centisome position: 29.12

GC content: 65.8

Gene sequence:

>690_bases
ATGCTGAACCAGTCGCTCAGGGCCCCGACCGTCGCCAAAACTCCGGTCGACCCTTCCTGCTCTCCGGCTGATCAGTTCTT
TGCAAGTACCGGCCACGCCGGCCTGATCGCCTCCGAGTTTTCCTACAAGAAGGAAGAGGAAATCTACGGCGAGGACGAGC
CCTCGGAATACGTCTACCAGGTCGTATCCGGCGCGGTCCGCAGCTACAAGCTGCTCTCCGACGGCCGCCGCCAGATCGGC
GCCTTCTATCTCCCCGGTGACGTGTTCGGGCTGGAATCGGGACCGACCCATCGCCTCACCGCGGAAGCCATCGTCGACAC
CACCGTTCGCCTGCTGAAGCGTCGCAGCCTCGAACAGGCCGCCGCCACCGACGTTCACGTCGCCCGCGGATTGTGGTCGA
TGACCGCCGGCGAGCTGCGCCACGCCGAAGACCACATGCTGCTGCTCGGCCGCAAGAATGCGATGGAGCGGGTCGCGAAC
TTCCTGCTGGAAATGGATCGCCGCCTCGCCGTTGCCGGCATGATGGCGTTGCCGATGTGCCGCCGCGACATCGGCGACTA
TCTCGGCCTCACCCTCGAGACCGTGTCGCGCGCGCTGTCGCAATTGCAGAGCCAGGGCATCCTCGGCTTCTCCGGCGCCC
GCCAGATCGAGCTGCGCAACCGCCAGCAGCTCCGCAACCTCGACGCCTGA

Upstream 100 bases:

>100_bases
TAGGTAAACCCCCTTAAGATATGGGGCGAAATACCAGGTCTGTACGATCTTCATTACAACCACGCCATCCGATCCAGATA
CCCCGATAGGAGATGGCAAG

Downstream 100 bases:

>100_bases
TCGCTTCAACGCAGAAGCGGTGGCGGCCCTCGCCATCGTTTCCGGCGTCATGCAACTCTCCGGAATTGGCCGCCAGAAAT
GGCGGCCTTTTTGTTGCGAC

Product: transcriptional regulator FixK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MLNQSLRAPTVAKTPVDPSCSPADQFFASTGHAGLIASEFSYKKEEEIYGEDEPSEYVYQVVSGAVRSYKLLSDGRRQIG
AFYLPGDVFGLESGPTHRLTAEAIVDTTVRLLKRRSLEQAAATDVHVARGLWSMTAGELRHAEDHMLLLGRKNAMERVAN
FLLEMDRRLAVAGMMALPMCRRDIGDYLGLTLETVSRALSQLQSQGILGFSGARQIELRNRQQLRNLDA

Sequences:

>Translated_229_residues
MLNQSLRAPTVAKTPVDPSCSPADQFFASTGHAGLIASEFSYKKEEEIYGEDEPSEYVYQVVSGAVRSYKLLSDGRRQIG
AFYLPGDVFGLESGPTHRLTAEAIVDTTVRLLKRRSLEQAAATDVHVARGLWSMTAGELRHAEDHMLLLGRKNAMERVAN
FLLEMDRRLAVAGMMALPMCRRDIGDYLGLTLETVSRALSQLQSQGILGFSGARQIELRNRQQLRNLDA
>Mature_229_residues
MLNQSLRAPTVAKTPVDPSCSPADQFFASTGHAGLIASEFSYKKEEEIYGEDEPSEYVYQVVSGAVRSYKLLSDGRRQIG
AFYLPGDVFGLESGPTHRLTAEAIVDTTVRLLKRRSLEQAAATDVHVARGLWSMTAGELRHAEDHMLLLGRKNAMERVAN
FLLEMDRRLAVAGMMALPMCRRDIGDYLGLTLETVSRALSQLQSQGILGFSGARQIELRNRQQLRNLDA

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK is a positive regulator of nifA expression. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=199, Percent_Identity=31.1557788944724, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25358; Mature: 25358

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQSLRAPTVAKTPVDPSCSPADQFFASTGHAGLIASEFSYKKEEEIYGEDEPSEYVYQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEHHHHCCCHHHHHCCCCCHHHHHHH
VVSGAVRSYKLLSDGRRQIGAFYLPGDVFGLESGPTHRLTAEAIVDTTVRLLKRRSLEQA
HHHHHHHHHHHHHHHHHHHCEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AATDVHVARGLWSMTAGELRHAEDHMLLLGRKNAMERVANFLLEMDRRLAVAGMMALPMC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRDIGDYLGLTLETVSRALSQLQSQGILGFSGARQIELRNRQQLRNLDA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNQSLRAPTVAKTPVDPSCSPADQFFASTGHAGLIASEFSYKKEEEIYGEDEPSEYVYQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEHHHHCCCHHHHHCCCCCHHHHHHH
VVSGAVRSYKLLSDGRRQIGAFYLPGDVFGLESGPTHRLTAEAIVDTTVRLLKRRSLEQA
HHHHHHHHHHHHHHHHHHHCEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AATDVHVARGLWSMTAGELRHAEDHMLLLGRKNAMERVANFLLEMDRRLAVAGMMALPMC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRDIGDYLGLTLETVSRALSQLQSQGILGFSGARQIELRNRQQLRNLDA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1766374 [H]