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The map label for this gene is mdeA [H]

Identifier: 86748485

GI number: 86748485

Start: 1550300

End: 1551607

Strand: Reverse

Name: mdeA [H]

Synonym: RPB_1360

Alternate gene names: 86748485

Gene position: 1551607-1550300 (Counterclockwise)

Preceding gene: 86748486

Following gene: 86748471

Centisome position: 29.1

GC content: 65.52

Gene sequence:

>1308_bases
ATGACCGAAAAGCTTCACCCTGAAACCCTCGCGCTGCACGCGGGCTGGCGCGCCGATCCTTCGACCGGCTCGGTCGCAGT
GCCGATCTTTCAAACGACGTCCTATCAATTCAACAACACAGAGCACGCGGCCAACCTGTTCGCACTGAAGGAACTCGGCA
ACATCTACACCCGGATCGGCAACCCAACGACCGACGTGCTGGAAAAGCGCGTCGCGGCGCTGGAAGGCGGCGTCGCGGCG
CTCGCAGTGGCGTCGGGCCAGGCGGCTTCGGCCTTTGCGATCCAGAACCTCGCTCGCGTCGGCGACAACGTCGTCAGTTC
GACCGATCTCTATGGCGGCACCTGGAATCTGTTCGCCAACACGCTGAAGGACCAGGGCATCGAAGTCCGCTTCGTCGATC
CGGCCGATCCGCAAGCCTTCGAGCGCGCCACCGACGACCGCACCCGCGCCTATTACGCCGAGACGCTGCCGAACCCGAAA
CTCGCGGTGTTTCCGATCGCCGAAGTGGCCGCGATCGGCCGCAAATTCGGCATTCCGCTGATCGTCGACAACACCGCCGC
GCCGTTGCTGGTGAAGCCGCTCGAACACGGCGCCGCGATCGTGGTGTATTCAGCGACCAAATATCTCGGCGGCCACGGCA
CCTCGATCGGCGGCTTGATCGTCGACGGCGGCAATTTCGACTGGGAGACATTCCCGCAGCGTCAGCCGGCGCTGAACACC
CCGGATCCGAGCTATCACGGCGCCGTCTGGGTCGAGGCGGTGAAGCCGATCGGCCCGGTCGCCTACATCATCAAGGCGCG
CACCACGCTGCTGCGCGACATCGGCTCGGCGCTGTCGCCGTTCAACGCCTTCCAGATCCTGCAGGGCCTGGAAACGCTGC
CGCTGCGAATCGAACGTCACGTGCAGAACGCCCAGGCGGTCGCCGACTATCTCGAAAAGCGGCCCGAGGTCGTCAAGGTG
ATCCATCCGTCGAAGCTGAGCGGCGTCGCGCGAGAGCGCGCCGACAAATACCTCAAGGGCAAGTTCGGCGGCCTGGTCGG
CTTCGAGCTCGCGGGCGGCCTCGAGGCCGGGCGCAAATTCATCGACGCGCTGCAACTGTTGTATCACGTCGCCAATATCG
GCGACGCGCGCAGCCTCGCGATCCATCCGGCGACGACCACGCACTCGCAGCTTTCCGCCGAGGACCAGCTCGCGACCGGC
GTGTCGGACGGCTATGTCCGGCTGTCGGTCGGCCTCGAACACATCGACGACATCATCGTCGATCTGGAGCGCGGCCTCGC
CGCGGGACGCCTCGCCAAGGCGGCGTAA

Upstream 100 bases:

>100_bases
CCCCCGACCTGATGGACGTCCCGGCGAACCTCAGCGTCGACCCGATGCAGACCCGACGCCACGACGGTTTCGCCTTTCAA
ATTTGTCGAAGGATTCCGTC

Downstream 100 bases:

>100_bases
GTTTGGACTAATATACGCTCCCTCTCCCCAAGCACAGCGCGCTCCCTCTCCCGCTTGCGGGAGAGGGTTGGGGTGAGGGC
GATACAAGCACTGACTCAGC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTEKLHPETLALHAGWRADPSTGSVAVPIFQTTSYQFNNTEHAANLFALKELGNIYTRIGNPTTDVLEKRVAALEGGVAA
LAVASGQAASAFAIQNLARVGDNVVSSTDLYGGTWNLFANTLKDQGIEVRFVDPADPQAFERATDDRTRAYYAETLPNPK
LAVFPIAEVAAIGRKFGIPLIVDNTAAPLLVKPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWETFPQRQPALNT
PDPSYHGAVWVEAVKPIGPVAYIIKARTTLLRDIGSALSPFNAFQILQGLETLPLRIERHVQNAQAVADYLEKRPEVVKV
IHPSKLSGVARERADKYLKGKFGGLVGFELAGGLEAGRKFIDALQLLYHVANIGDARSLAIHPATTTHSQLSAEDQLATG
VSDGYVRLSVGLEHIDDIIVDLERGLAAGRLAKAA

Sequences:

>Translated_435_residues
MTEKLHPETLALHAGWRADPSTGSVAVPIFQTTSYQFNNTEHAANLFALKELGNIYTRIGNPTTDVLEKRVAALEGGVAA
LAVASGQAASAFAIQNLARVGDNVVSSTDLYGGTWNLFANTLKDQGIEVRFVDPADPQAFERATDDRTRAYYAETLPNPK
LAVFPIAEVAAIGRKFGIPLIVDNTAAPLLVKPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWETFPQRQPALNT
PDPSYHGAVWVEAVKPIGPVAYIIKARTTLLRDIGSALSPFNAFQILQGLETLPLRIERHVQNAQAVADYLEKRPEVVKV
IHPSKLSGVARERADKYLKGKFGGLVGFELAGGLEAGRKFIDALQLLYHVANIGDARSLAIHPATTTHSQLSAEDQLATG
VSDGYVRLSVGLEHIDDIIVDLERGLAAGRLAKAA
>Mature_434_residues
TEKLHPETLALHAGWRADPSTGSVAVPIFQTTSYQFNNTEHAANLFALKELGNIYTRIGNPTTDVLEKRVAALEGGVAAL
AVASGQAASAFAIQNLARVGDNVVSSTDLYGGTWNLFANTLKDQGIEVRFVDPADPQAFERATDDRTRAYYAETLPNPKL
AVFPIAEVAAIGRKFGIPLIVDNTAAPLLVKPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWETFPQRQPALNTP
DPSYHGAVWVEAVKPIGPVAYIIKARTTLLRDIGSALSPFNAFQILQGLETLPLRIERHVQNAQAVADYLEKRPEVVKVI
HPSKLSGVARERADKYLKGKFGGLVGFELAGGLEAGRKFIDALQLLYHVANIGDARSLAIHPATTTHSQLSAEDQLATGV
SDGYVRLSVGLEHIDDIIVDLERGLAAGRLAKAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=410, Percent_Identity=33.1707317073171, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI25453487, Length=408, Percent_Identity=29.1666666666667, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI299473759, Length=410, Percent_Identity=30, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1790375, Length=431, Percent_Identity=32.4825986078886, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=25.1748251748252, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538055, Length=414, Percent_Identity=32.3671497584541, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=420, Percent_Identity=30.2380952380952, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=413, Percent_Identity=32.2033898305085, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=47.1264367816092, Blast_Score=346, Evalue=4e-96,
Organism=Saccharomyces cerevisiae, GI6319307, Length=425, Percent_Identity=31.5294117647059, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321254, Length=452, Percent_Identity=26.1061946902655, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=27.0186335403727, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922554, Length=435, Percent_Identity=32.183908045977, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46565; Mature: 46434

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKLHPETLALHAGWRADPSTGSVAVPIFQTTSYQFNNTEHAANLFALKELGNIYTRIG
CCCCCCCCEEEEECCCCCCCCCCCEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCC
NPTTDVLEKRVAALEGGVAALAVASGQAASAFAIQNLARVGDNVVSSTDLYGGTWNLFAN
CCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHH
TLKDQGIEVRFVDPADPQAFERATDDRTRAYYAETLPNPKLAVFPIAEVAAIGRKFGIPL
HHHCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCE
IVDNTAAPLLVKPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWETFPQRQPALNT
EECCCCCCEEEEECCCCCEEEEEECHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PDPSYHGAVWVEAVKPIGPVAYIIKARTTLLRDIGSALSPFNAFQILQGLETLPLRIERH
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHH
VQNAQAVADYLEKRPEVVKVIHPSKLSGVARERADKYLKGKFGGLVGFELAGGLEAGRKF
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHH
IDALQLLYHVANIGDARSLAIHPATTTHSQLSAEDQLATGVSDGYVRLSVGLEHIDDIIV
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCCEEEEEECHHHHHHHHH
DLERGLAAGRLAKAA
HHHHCHHHHHHHHCC
>Mature Secondary Structure 
TEKLHPETLALHAGWRADPSTGSVAVPIFQTTSYQFNNTEHAANLFALKELGNIYTRIG
CCCCCCCEEEEECCCCCCCCCCCEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCC
NPTTDVLEKRVAALEGGVAALAVASGQAASAFAIQNLARVGDNVVSSTDLYGGTWNLFAN
CCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHH
TLKDQGIEVRFVDPADPQAFERATDDRTRAYYAETLPNPKLAVFPIAEVAAIGRKFGIPL
HHHCCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCE
IVDNTAAPLLVKPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWETFPQRQPALNT
EECCCCCCEEEEECCCCCEEEEEECHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PDPSYHGAVWVEAVKPIGPVAYIIKARTTLLRDIGSALSPFNAFQILQGLETLPLRIERH
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHH
VQNAQAVADYLEKRPEVVKVIHPSKLSGVARERADKYLKGKFGGLVGFELAGGLEAGRKF
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHH
IDALQLLYHVANIGDARSLAIHPATTTHSQLSAEDQLATGVSDGYVRLSVGLEHIDDIIV
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCCEEEEEECHHHHHHHHH
DLERGLAAGRLAKAA
HHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]