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The map label for this gene is nuoN [H]

Identifier: 86748484

GI number: 86748484

Start: 1548771

End: 1550132

Strand: Direct

Name: nuoN [H]

Synonym: RPB_1359

Alternate gene names: 86748484

Gene position: 1548771-1550132 (Clockwise)

Preceding gene: 86748483

Following gene: 86748492

Centisome position: 29.05

GC content: 69.6

Gene sequence:

>1362_bases
ATGACCGCAGCCCAGTTCGCCGCGCTTTCGCCGCTCATCGCGCTGGCCATCGCCGCTGTCGCAGCGATGATGCTGGTGCC
GCTGGCGCGCATCGAAGCCGCGCAGGCGGCGGCTGCCGCGGGCCTGGTTGCGGCTGTGACGCTGGTGCTGCTGCGGATCG
GCGCGCCGTCGGCGCCGCTGGGTGCGCTGTTCACCGACGACGGCATCGCCCGGTTCGGCGTGGCCTATGTCTGTCTCGCC
TCGCTGGGCGCGTTGATCTTCCTGCGCACCGGCCACCCCGCCAAGGAGGCGCCGTCGCTGCTGGTGCTGGTTGCGCTCGG
CGCAGCGACGCTGGCGGGAGCCGGCCATGCGGCGACGCTGTTTCTCGGCATCGAGATCATCAGCCTGTCGCTGATCGCAT
TGTTCGCCTACCGGTTGACGCGGCAGGGGCTGGAAGCCGCCTACAAGTTTCTGGTGATGAGCGGGCTGGCCTCGGCGGCG
CTGCTGCTCGGCACAGCCCTGATCTATGCCGAGACCGGCGCGCTCGATTTCACCGGCTGGACCGGGCACGGCGCGCTGGC
CGCGCTCGGCACCGCGCTGCTGCTCGCGGGTCTTGCGTTCAAGTTCTCGCTGGCGCCGTTCCACATGTGGACGCCCGATG
CGTTCGAAGCCGCGCCCGCGAGCGCCGCGGCGCTGGCCGGCGTGGTGTCGAAAGCCGCTGTGGCGCTCGTGTTGCTGCGG
CTGTTTCTCACTGCGCACCTGCCGGAGCCGGTGTGGAGCTCGGGCCTCGCCGCGCTCGGCGTCGCCTCGGTGGTGGTCGG
CAATCTGCTGGCTCTGCGCCAACCGCAATTGCCGCGGATGCTCGGCTATTCGACCATCGCGCATTCCGGCTACATCGCGC
TGATCCTGGCCTCGGGCGCCCCGCGCGTCGCGGAGGCGGTGCTGTTCTATCTCGCCGTCTACGCGCCGGCGATGCTCGGC
GTGCTGTGCGTCTCGGCCGCGCTCGGCCGCGCGCCGACGTTGTACGATCTGCACGGGCTGATCAAGCGCCGCCCGCTGGA
GGCGATCGCGCTGTCGCTGTCGTTGCTGTCGCTGGCGGGATTGCCGGCCGCGGGCGGCTTCATCGGCAAATTATATCTGC
TGCACGTACTGGTCGATCGCGGCCTATGGACGCTGCTGGCGATCACCGTCGCCGGCGCGTCGCTCGGCTTCTATTACTAC
GCACGGTTCTTCACCGCGCCGTTCTTCGGCCATCTGCACGAAGAGGCCGAGCCGTTGCCGCGGCTCGATCAGGGGCTGAT
CGCTGTGTGCGTGGTGCTGATCATCCTGTTCGGCGTCGCGCCGGCGGTGCTGATCGATGTGGTGAGGCTCGCCCTGCCTT
AA

Upstream 100 bases:

>100_bases
TGGCGATGGGCCTCGCCACGCTGGTAATCGGCCTGTATCCGCAGAGCCTGCTGGCGCTGCTGGAGCCCGCGATCAAGGCC
GCTCTGGTGCTGCCGCAGCC

Downstream 100 bases:

>100_bases
CCTCTCCCGCTTGCGGGAGAGGTCGGATCGCGCAGCGATCCGGGTGAGGGCACTCCGCTCCATGCTTGCTGAGTCAGTGC
TTGTATCGCCCTCACCCCAA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTAAQFAALSPLIALAIAAVAAMMLVPLARIEAAQAAAAAGLVAAVTLVLLRIGAPSAPLGALFTDDGIARFGVAYVCLA
SLGALIFLRTGHPAKEAPSLLVLVALGAATLAGAGHAATLFLGIEIISLSLIALFAYRLTRQGLEAAYKFLVMSGLASAA
LLLGTALIYAETGALDFTGWTGHGALAALGTALLLAGLAFKFSLAPFHMWTPDAFEAAPASAAALAGVVSKAAVALVLLR
LFLTAHLPEPVWSSGLAALGVASVVVGNLLALRQPQLPRMLGYSTIAHSGYIALILASGAPRVAEAVLFYLAVYAPAMLG
VLCVSAALGRAPTLYDLHGLIKRRPLEAIALSLSLLSLAGLPAAGGFIGKLYLLHVLVDRGLWTLLAITVAGASLGFYYY
ARFFTAPFFGHLHEEAEPLPRLDQGLIAVCVVLIILFGVAPAVLIDVVRLALP

Sequences:

>Translated_453_residues
MTAAQFAALSPLIALAIAAVAAMMLVPLARIEAAQAAAAAGLVAAVTLVLLRIGAPSAPLGALFTDDGIARFGVAYVCLA
SLGALIFLRTGHPAKEAPSLLVLVALGAATLAGAGHAATLFLGIEIISLSLIALFAYRLTRQGLEAAYKFLVMSGLASAA
LLLGTALIYAETGALDFTGWTGHGALAALGTALLLAGLAFKFSLAPFHMWTPDAFEAAPASAAALAGVVSKAAVALVLLR
LFLTAHLPEPVWSSGLAALGVASVVVGNLLALRQPQLPRMLGYSTIAHSGYIALILASGAPRVAEAVLFYLAVYAPAMLG
VLCVSAALGRAPTLYDLHGLIKRRPLEAIALSLSLLSLAGLPAAGGFIGKLYLLHVLVDRGLWTLLAITVAGASLGFYYY
ARFFTAPFFGHLHEEAEPLPRLDQGLIAVCVVLIILFGVAPAVLIDVVRLALP
>Mature_452_residues
TAAQFAALSPLIALAIAAVAAMMLVPLARIEAAQAAAAAGLVAAVTLVLLRIGAPSAPLGALFTDDGIARFGVAYVCLAS
LGALIFLRTGHPAKEAPSLLVLVALGAATLAGAGHAATLFLGIEIISLSLIALFAYRLTRQGLEAAYKFLVMSGLASAAL
LLGTALIYAETGALDFTGWTGHGALAALGTALLLAGLAFKFSLAPFHMWTPDAFEAAPASAAALAGVVSKAAVALVLLRL
FLTAHLPEPVWSSGLAALGVASVVVGNLLALRQPQLPRMLGYSTIAHSGYIALILASGAPRVAEAVLFYLAVYAPAMLGV
LCVSAALGRAPTLYDLHGLIKRRPLEAIALSLSLLSLAGLPAAGGFIGKLYLLHVLVDRGLWTLLAITVAGASLGFYYYA
RFFTAPFFGHLHEEAEPLPRLDQGLIAVCVVLIILFGVAPAVLIDVVRLALP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=485, Percent_Identity=37.319587628866, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1788831, Length=270, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788827, Length=271, Percent_Identity=28.0442804428044, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46459; Mature: 46328

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAQFAALSPLIALAIAAVAAMMLVPLARIEAAQAAAAAGLVAAVTLVLLRIGAPSAPL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GALFTDDGIARFGVAYVCLASLGALIFLRTGHPAKEAPSLLVLVALGAATLAGAGHAATL
CCEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
FLGIEIISLSLIALFAYRLTRQGLEAAYKFLVMSGLASAALLLGTALIYAETGALDFTGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
TGHGALAALGTALLLAGLAFKFSLAPFHMWTPDAFEAAPASAAALAGVVSKAAVALVLLR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
LFLTAHLPEPVWSSGLAALGVASVVVGNLLALRQPQLPRMLGYSTIAHSGYIALILASGA
HHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCC
PRVAEAVLFYLAVYAPAMLGVLCVSAALGRAPTLYDLHGLIKRRPLEAIALSLSLLSLAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHC
LPAAGGFIGKLYLLHVLVDRGLWTLLAITVAGASLGFYYYARFFTAPFFGHLHEEAEPLP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RLDQGLIAVCVVLIILFGVAPAVLIDVVRLALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAAQFAALSPLIALAIAAVAAMMLVPLARIEAAQAAAAAGLVAAVTLVLLRIGAPSAPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GALFTDDGIARFGVAYVCLASLGALIFLRTGHPAKEAPSLLVLVALGAATLAGAGHAATL
CCEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
FLGIEIISLSLIALFAYRLTRQGLEAAYKFLVMSGLASAALLLGTALIYAETGALDFTGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
TGHGALAALGTALLLAGLAFKFSLAPFHMWTPDAFEAAPASAAALAGVVSKAAVALVLLR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
LFLTAHLPEPVWSSGLAALGVASVVVGNLLALRQPQLPRMLGYSTIAHSGYIALILASGA
HHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCC
PRVAEAVLFYLAVYAPAMLGVLCVSAALGRAPTLYDLHGLIKRRPLEAIALSLSLLSLAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHC
LPAAGGFIGKLYLLHVLVDRGLWTLLAITVAGASLGFYYYARFFTAPFFGHLHEEAEPLP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RLDQGLIAVCVVLIILFGVAPAVLIDVVRLALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA