BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is dkgB [H]

Identifier: 86748454

GI number: 86748454

Start: 1515530

End: 1516354

Strand: Reverse

Name: dkgB [H]

Synonym: RPB_1329

Alternate gene names: 86748454

Gene position: 1516354-1515530 (Counterclockwise)

Preceding gene: 86748457

Following gene: 86748453

Centisome position: 28.44

GC content: 64.61

Gene sequence:

>825_bases
ATGGAATCACTGACCACGCAGGGCCTGAATGTGCCCCGACTCGGACTGGGCACCTTCAAGCTGCAGGGCAAGGATTGCCA
CGAAGCGGTGCTGAGCGCGCTGGCGATGGGCTATCGCCATATCGACACCGCCGAGATGTACGCCAACGAGGATGCGGTCG
GCGACGCGCTCGAGGACACCGCGGTGCCGCGCGAGCAGATCCACCTCACCACCAAAGTGTGGTGGACCAATCTCGCCCCC
GACGCGATCCGCAAGGCGTTCGACCAGAGCTTGGCCAAGCTGAAGACCGACTATGTCGACTTCTATCTGATCCATTGGCC
GGCGCCCGACATGAATCTCGCAAGCGCGCTGGAGACGCTGATGCGGATCAGGGACGACGGCGGCGCCCGCGCCATCGGCG
TCTGCAATTTTCCAATCGCGCTGCTGAAACAGGCGATCGAGGACATCAATGCGCCGATCGCCTGCAACCAGGTCGAGTAT
CACGCGCTGCTCGGCCAATCGAAGCTGCTGCCCTATATGCAGAGCAAGGGCATGCCGCTGACCGCCTATGCGCCGCTGGC
GCAGGGCCGGCTCGCCTCGTTTCCGGAGTTGAAGGCGATCGCCGACAAGCACGACGCCACCGCGGCGCAGATCGCGCTGG
CCTGGCTGCTCGAGCAGGACGGCGTGATGGCGATCCCGAAGGCGCGCAGCGAGGCCAGCCAGAAGAGCAATCTCGGCGCG
CTCGACATCAAACTCGACCAGGACGATCGCGCGCTGATCGCCAAGCTGCCGAAGAACCAGCGCTTCGTCACGCCGGCGTT
CTCGCCGGAATGGGACGAGGAGTAG

Upstream 100 bases:

>100_bases
CGGCCTCGCGCAACCCTTGACGGCGACTTTCGTTACCGCTTGGCTGACTTGATGCGCAGCAGAGCCGCCACCCGGCGCTC
ACAGGGAGACGGCGATCGCC

Downstream 100 bases:

>100_bases
AGCCCTCCGCGCTGAGAGCCTGACGAAAACTGCAGGCAGCAAAATCAGCCGCGTCTGAAAGTCGTTCACTTCGCAATTCG
CGCCGCCATGGCCGGGCTCG

Product: aldo/keto reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MESLTTQGLNVPRLGLGTFKLQGKDCHEAVLSALAMGYRHIDTAEMYANEDAVGDALEDTAVPREQIHLTTKVWWTNLAP
DAIRKAFDQSLAKLKTDYVDFYLIHWPAPDMNLASALETLMRIRDDGGARAIGVCNFPIALLKQAIEDINAPIACNQVEY
HALLGQSKLLPYMQSKGMPLTAYAPLAQGRLASFPELKAIADKHDATAAQIALAWLLEQDGVMAIPKARSEASQKSNLGA
LDIKLDQDDRALIAKLPKNQRFVTPAFSPEWDEE

Sequences:

>Translated_274_residues
MESLTTQGLNVPRLGLGTFKLQGKDCHEAVLSALAMGYRHIDTAEMYANEDAVGDALEDTAVPREQIHLTTKVWWTNLAP
DAIRKAFDQSLAKLKTDYVDFYLIHWPAPDMNLASALETLMRIRDDGGARAIGVCNFPIALLKQAIEDINAPIACNQVEY
HALLGQSKLLPYMQSKGMPLTAYAPLAQGRLASFPELKAIADKHDATAAQIALAWLLEQDGVMAIPKARSEASQKSNLGA
LDIKLDQDDRALIAKLPKNQRFVTPAFSPEWDEE
>Mature_274_residues
MESLTTQGLNVPRLGLGTFKLQGKDCHEAVLSALAMGYRHIDTAEMYANEDAVGDALEDTAVPREQIHLTTKVWWTNLAP
DAIRKAFDQSLAKLKTDYVDFYLIHWPAPDMNLASALETLMRIRDDGGARAIGVCNFPIALLKQAIEDINAPIACNQVEY
HALLGQSKLLPYMQSKGMPLTAYAPLAQGRLASFPELKAIADKHDATAAQIALAWLLEQDGVMAIPKARSEASQKSNLGA
LDIKLDQDDRALIAKLPKNQRFVTPAFSPEWDEE

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=33.7883959044369, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI310109922, Length=277, Percent_Identity=34.6570397111913, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI310109920, Length=296, Percent_Identity=33.1081081081081, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI24497585, Length=299, Percent_Identity=32.7759197324415, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI45446745, Length=296, Percent_Identity=33.1081081081081, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4503285, Length=296, Percent_Identity=33.1081081081081, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI5453543, Length=296, Percent_Identity=32.7702702702703, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI24497577, Length=299, Percent_Identity=29.7658862876254, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI5174391, Length=299, Percent_Identity=29.7658862876254, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI24497583, Length=296, Percent_Identity=32.7702702702703, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI291291012, Length=269, Percent_Identity=33.0855018587361, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI5174695, Length=301, Percent_Identity=31.8936877076412, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI4502049, Length=293, Percent_Identity=31.740614334471, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI300116273, Length=274, Percent_Identity=31.3868613138686, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=31.7689530685921, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI93277124, Length=301, Percent_Identity=28.2392026578073, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI310109926, Length=108, Percent_Identity=40.7407407407407, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI207028673, Length=108, Percent_Identity=40.7407407407407, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI310109924, Length=156, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786400, Length=266, Percent_Identity=39.8496240601504, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI87082198, Length=258, Percent_Identity=36.046511627907, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1788081, Length=266, Percent_Identity=34.2105263157895, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788070, Length=319, Percent_Identity=26.0188087774295, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081735, Length=294, Percent_Identity=29.2517006802721, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787674, Length=275, Percent_Identity=25.4545454545455, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=30.7692307692308, Blast_Score=137, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17566692, Length=282, Percent_Identity=30.1418439716312, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17550248, Length=294, Percent_Identity=30.952380952381, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=30, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=32.1167883211679, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17537075, Length=295, Percent_Identity=30.8474576271186, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17552492, Length=258, Percent_Identity=29.0697674418605, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=30, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17538386, Length=263, Percent_Identity=31.5589353612167, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17562292, Length=287, Percent_Identity=28.2229965156794, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=24.5791245791246, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324694, Length=300, Percent_Identity=35.3333333333333, Blast_Score=165, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6320576, Length=286, Percent_Identity=33.5664335664336, Blast_Score=152, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319625, Length=265, Percent_Identity=31.6981132075472, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=28.8461538461538, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321896, Length=325, Percent_Identity=26.4615384615385, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320079, Length=241, Percent_Identity=29.045643153527, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24657054, Length=286, Percent_Identity=31.4685314685315, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24644950, Length=303, Percent_Identity=31.3531353135314, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=29.3103448275862, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI221378297, Length=323, Percent_Identity=29.4117647058824, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24662789, Length=300, Percent_Identity=32, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI20129731, Length=285, Percent_Identity=32.280701754386, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45553081, Length=275, Percent_Identity=32, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24663317, Length=290, Percent_Identity=28.9655172413793, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=30.4498269896194, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=30.2405498281787, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281366140, Length=241, Percent_Identity=31.1203319502075, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 30154; Mature: 30154

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLTTQGLNVPRLGLGTFKLQGKDCHEAVLSALAMGYRHIDTAEMYANEDAVGDALEDT
CCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCHHHHH
AVPREQIHLTTKVWWTNLAPDAIRKAFDQSLAKLKTDYVDFYLIHWPAPDMNLASALETL
CCCHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHH
MRIRDDGGARAIGVCNFPIALLKQAIEDINAPIACNQVEYHALLGQSKLLPYMQSKGMPL
HHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEECCCHHHHHHCCHHCCHHHHHCCCCE
TAYAPLAQGRLASFPELKAIADKHDATAAQIALAWLLEQDGVMAIPKARSEASQKSNLGA
EEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCCE
LDIKLDQDDRALIAKLPKNQRFVTPAFSPEWDEE
EEEEECCCCCHHHHHCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MESLTTQGLNVPRLGLGTFKLQGKDCHEAVLSALAMGYRHIDTAEMYANEDAVGDALEDT
CCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCHHHHH
AVPREQIHLTTKVWWTNLAPDAIRKAFDQSLAKLKTDYVDFYLIHWPAPDMNLASALETL
CCCHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHH
MRIRDDGGARAIGVCNFPIALLKQAIEDINAPIACNQVEYHALLGQSKLLPYMQSKGMPL
HHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEECCCHHHHHHCCHHCCHHHHHCCCCE
TAYAPLAQGRLASFPELKAIADKHDATAAQIALAWLLEQDGVMAIPKARSEASQKSNLGA
EEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCCE
LDIKLDQDDRALIAKLPKNQRFVTPAFSPEWDEE
EEEEECCCCCHHHHHCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]