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The map label for this gene is pleD [H]

Identifier: 86748375

GI number: 86748375

Start: 1428795

End: 1429724

Strand: Reverse

Name: pleD [H]

Synonym: RPB_1250

Alternate gene names: 86748375

Gene position: 1429724-1428795 (Counterclockwise)

Preceding gene: 86748376

Following gene: 86748369

Centisome position: 26.82

GC content: 67.96

Gene sequence:

>930_bases
ATGACCAATGATCTGAAGAAGCGCGATATCGTGCTCGTCGTCGACGACTCGCCGGAGACGCTGCGGATGCTGACCGACGC
GCTCGACGGCGCCGGCATGACCGTGATGGTCGCGCTCGACGGCGCCGCGGCGATGCGGATCGTCGAGCAGATCACGCCGG
ACATCGTCCTGCTCGACGCCATCATGCCGGGCCTCGACGGCTTCGAGACCTGCCGGCGGCTGAAGCGCAATCCCGCCGTC
TCCGACGTGCCGGTGATCTTCATGACCGGCCTCGCCGAGAGCGAGCACATCGTCCGCGGCCTGGAGGCCGGCGGCGTCGA
CTACGTCACCAAGCCGATCGTGATCGTCGAAATGCTGGCGCGGATCCGGGTGCATCTCGGCAATGCGCGGCTGAGCCAGA
GCGCGCGCGCCGCACTCGACGTGTCCGGCCGCTTCCTGCTCGCGGTCGATCGCGTCGGCAAGGTGCTGTGGGCGACGCCG
CAGGCGCAGAAGCTGCTCGCCGCCGACGGCGCCTCGGCCGAAGGCTTCCGGCTCGATCTGCCGAAGCCCTGGCTCGACTG
GCTGGAGCAGGTGCACAGCGGCCTCGCCCAGCCGAAGACGCCGATGAAGGCGTCGCTGCTGCGCAACGACGAATTGCGGC
TGCAATATATGGGCAAGCTCGGGCCGAACGAATTCCTGCTGCGGCTGGCGCGCGACACCTCCGGCGAATCGCCGAAGGAA
TTCACCACCGAGTTCGGTCTCACCAGCCGCGAGAGCGAAGTGCTGGCGTGGCTCAGCAAGGGCAAGACCAATCGCGACAT
CGCGCAGATTCTGGGGCTCAGCCCGCGCACGGTCGACAAGCACCTCGAGCAGATCTACGCCAAGCTCGGCGTCGAAAACC
GCACCGCGGCGGCGGCGATCGCCACCAGCGCCAGCCGGCGGGATTCGTAA

Upstream 100 bases:

>100_bases
GACTATCCGGAATCGGGGACGTTCGTGTCGCAGATGCGCGCGCTGGTCGAGCAGTTCGATCTCGACCAGTACATGAAGAC
CCTGAACACGCTGTATCGCC

Downstream 100 bases:

>100_bases
GCCGCCGCGCGTCGCCGCGGCGCGATCAGCAGGGTTTCGTGGAACTGCTCAGCGTGCGGACGAACGCTGTCTGCGGCCGC
CCTTGGCCTGCATCTCGATC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTNDLKKRDIVLVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIVLLDAIMPGLDGFETCRRLKRNPAV
SDVPVIFMTGLAESEHIVRGLEAGGVDYVTKPIVIVEMLARIRVHLGNARLSQSARAALDVSGRFLLAVDRVGKVLWATP
QAQKLLAADGASAEGFRLDLPKPWLDWLEQVHSGLAQPKTPMKASLLRNDELRLQYMGKLGPNEFLLRLARDTSGESPKE
FTTEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAIATSASRRDS

Sequences:

>Translated_309_residues
MTNDLKKRDIVLVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIVLLDAIMPGLDGFETCRRLKRNPAV
SDVPVIFMTGLAESEHIVRGLEAGGVDYVTKPIVIVEMLARIRVHLGNARLSQSARAALDVSGRFLLAVDRVGKVLWATP
QAQKLLAADGASAEGFRLDLPKPWLDWLEQVHSGLAQPKTPMKASLLRNDELRLQYMGKLGPNEFLLRLARDTSGESPKE
FTTEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAIATSASRRDS
>Mature_308_residues
TNDLKKRDIVLVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIVLLDAIMPGLDGFETCRRLKRNPAVS
DVPVIFMTGLAESEHIVRGLEAGGVDYVTKPIVIVEMLARIRVHLGNARLSQSARAALDVSGRFLLAVDRVGKVLWATPQ
AQKLLAADGASAEGFRLDLPKPWLDWLEQVHSGLAQPKTPMKASLLRNDELRLQYMGKLGPNEFLLRLARDTSGESPKEF
TTEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAIATSASRRDS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=162, Percent_Identity=32.7160493827161, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=37.1681415929204, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790299, Length=131, Percent_Identity=34.3511450381679, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=34.4827586206897, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082012, Length=133, Percent_Identity=29.3233082706767, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=34.7107438016529, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786911, Length=116, Percent_Identity=37.0689655172414, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=34.7457627118644, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=106, Percent_Identity=34.9056603773585, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787229, Length=126, Percent_Identity=31.7460317460317, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33747; Mature: 33616

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDLKKRDIVLVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIVLLDA
CCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCEEEHHH
IMPGLDGFETCRRLKRNPAVSDVPVIFMTGLAESEHIVRGLEAGGVDYVTKPIVIVEMLA
HHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RIRVHLGNARLSQSARAALDVSGRFLLAVDRVGKVLWATPQAQKLLAADGASAEGFRLDL
HHHHHHCCHHHHHHHHHHHCCCCCEEEEHHHHCHHEECCCHHHHHHHCCCCCCCCEEECC
PKPWLDWLEQVHSGLAQPKTPMKASLLRNDELRLQYMGKLGPNEFLLRLARDTSGESPKE
CCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
FTTEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAI
HHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
ATSASRRDS
HHHHHCCCC
>Mature Secondary Structure 
TNDLKKRDIVLVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIVLLDA
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCEEEHHH
IMPGLDGFETCRRLKRNPAVSDVPVIFMTGLAESEHIVRGLEAGGVDYVTKPIVIVEMLA
HHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RIRVHLGNARLSQSARAALDVSGRFLLAVDRVGKVLWATPQAQKLLAADGASAEGFRLDL
HHHHHHCCHHHHHHHHHHHCCCCCEEEEHHHHCHHEECCCHHHHHHHCCCCCCCCEEECC
PKPWLDWLEQVHSGLAQPKTPMKASLLRNDELRLQYMGKLGPNEFLLRLARDTSGESPKE
CCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
FTTEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAI
HHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
ATSASRRDS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA