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The map label for this gene is phoB [H]

Identifier: 86747907

GI number: 86747907

Start: 874529

End: 875230

Strand: Direct

Name: phoB [H]

Synonym: RPB_0781

Alternate gene names: 86747907

Gene position: 874529-875230 (Clockwise)

Preceding gene: 86747906

Following gene: 86747909

Centisome position: 16.4

GC content: 67.81

Gene sequence:

>702_bases
ATGGGTGCGCGCATTCTGGTGGTTGAAGACGAGGAAGCGCTGACCACGCTGCTGCGATACAATCTCGAATCCGACGGCTA
CGACGTCGAAACCGTCGCCCGCGGCGACGATGCCGACACACGGATGAAGGAGCGGCTGCCGGATCTGGTGGTGCTGGACT
GGATGCTGCCGGGTCTCTCCGGCATCGAACTGTGCCGCCGGCTGCGGGCGCGGACCGACACCAAGCAGTTGCCGATCATC
ATGTTGACCGCGCGCGGCGAGGAGAGCGAACGCGTCCGCGGCCTCGCCACCGGCGCCGACGACTACATCGTCAAGCCGTT
CTCGGTGCCGGAACTGCTGGCGCGGGTGAAGGGTCTGCTGCGTCGCGCCGCACCCGAGCGGCTCGCCACCGTGCTGACCT
ATGGCGATCTCGAACTCGACCGCGACAAGCGCCGCGTCGCGCGCAGCGGCCGGCCGATCGATCTCGGCCCGACCGAATAT
CGCCTGCTCGAATTCTTCCTGGAGCATCCCGGCCGGGTGTTCAGCCGCGAGCAACTGCTCGACGGCGTCTGGGGCCGCGA
CATCTACATCGACGAGCGCACCGTCGACGTCCACATCGGCCGTCTGCGCAAGCTGCTCAATCCGGGCCGCGAGCAGGACC
CGATCCGCACCGTGCGCGGCGCCGGCTACGCGCTCGACGATCGCTTCGCCAAGGTGGAGTAA

Upstream 100 bases:

>100_bases
CATGACGACTTTCGCCAACGCGACGCCGGGCAACTGACGCGCAGGACGGCGGGATGCGGAGCGCATCCCGGATACGCTCG
ACTGAAGACGAGGACGACAC

Downstream 100 bases:

>100_bases
GCGCGAAGCGCTTCAACAGGAGCAAGTCGGTAGCCCGCATGAGCGCATCGCCATGCGGGGTTCGAGCGGTGCCTCCCGCA
TATCGCTGCGCTCATGCGGG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MGARILVVEDEEALTTLLRYNLESDGYDVETVARGDDADTRMKERLPDLVVLDWMLPGLSGIELCRRLRARTDTKQLPII
MLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLLRRAAPERLATVLTYGDLELDRDKRRVARSGRPIDLGPTEY
RLLEFFLEHPGRVFSREQLLDGVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKVE

Sequences:

>Translated_233_residues
MGARILVVEDEEALTTLLRYNLESDGYDVETVARGDDADTRMKERLPDLVVLDWMLPGLSGIELCRRLRARTDTKQLPII
MLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLLRRAAPERLATVLTYGDLELDRDKRRVARSGRPIDLGPTEY
RLLEFFLEHPGRVFSREQLLDGVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKVE
>Mature_232_residues
GARILVVEDEEALTTLLRYNLESDGYDVETVARGDDADTRMKERLPDLVVLDWMLPGLSGIELCRRLRARTDTKQLPIIM
LTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLLRRAAPERLATVLTYGDLELDRDKRRVARSGRPIDLGPTEYR
LLEFFLEHPGRVFSREQLLDGVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKVE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=49.7816593886463, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=43.2432432432432, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1790860, Length=229, Percent_Identity=41.9213973799127, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=39.4849785407725, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=40.3587443946188, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1786911, Length=232, Percent_Identity=39.2241379310345, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787375, Length=226, Percent_Identity=34.070796460177, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=34.6666666666667, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=36.036036036036, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=36.4864864864865, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=36.9565217391304, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=36.2068965517241, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=34.4978165938865, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=33.9055793991416, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=28.3333333333333, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=31.1926605504587, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26570; Mature: 26439

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGARILVVEDEEALTTLLRYNLESDGYDVETVARGDDADTRMKERLPDLVVLDWMLPGLS
CCCEEEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEHHHHHCCCC
GIELCRRLRARTDTKQLPIIMLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLL
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRAAPERLATVLTYGDLELDRDKRRVARSGRPIDLGPTEYRLLEFFLEHPGRVFSREQLL
HHHCHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
DGVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKVE
CCCCCCEEEEECCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHCCC
>Mature Secondary Structure 
GARILVVEDEEALTTLLRYNLESDGYDVETVARGDDADTRMKERLPDLVVLDWMLPGLS
CCEEEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEHHHHHCCCC
GIELCRRLRARTDTKQLPIIMLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLL
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRAAPERLATVLTYGDLELDRDKRRVARSGRPIDLGPTEYRLLEFFLEHPGRVFSREQLL
HHHCHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
DGVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKVE
CCCCCCEEEEECCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]