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The map label for this gene is ispD [H]

Identifier: 86610132

GI number: 86610132

Start: 2795623

End: 2796291

Strand: Direct

Name: ispD [H]

Synonym: CYB_2706

Alternate gene names: 86610132

Gene position: 2795623-2796291 (Clockwise)

Preceding gene: 86610131

Following gene: 86610133

Centisome position: 91.76

GC content: 59.04

Gene sequence:

>669_bases
ATGGGAGCTGCGGTCAATAAGCTGTTGCTGCCGCTCTTGGGTCAGCCGATTTTGGCTTGGACGTTGCAGGCTGCGGATCA
GGCCAGCTCCATCGAGTGGATCGGCATTCTCTCCCAACCCGGCGATTGGCCAGCCATCGAGCAGATCCTGGCGGCAATGG
ATCTGTCCACGCCGGTGCAGCTTTTACAAGGGGGGGCGACGCGGCAGGAGTCGGTTTACAGGGGGGTTGAGTATCTCTAT
AACCTGGGAACAGTAGAGCGGGTGTTGATCCACGATGGGGCCCGCTGCCTAGCTACCCCTAAGTTGTTCGATCGCTGTAG
TGCTGCTTTGCAGCAGGTGGATGGTCTCATCGCGGGGATCCCTGTCAAGGACACGATTAAAGTCGCTGAGGCAGGCCCAA
AAGGGATCCAAATTCAGTCAACCCCAGAACGCAGCCGGCTGTGGGCAGCGCAAACGCCGCAGGGGTTTCGCCTGTCGATG
CTATGGCAAGCCCATCGGGAGGCTCTGGCCCAGGGTTGGCAGGTAACCGATGATGCAGCCCTATTCGAGAAGCTGGGTTG
GCCAGTCTACATCGTCGAAGGGGAGGAATCCAATCTGAAGCTAACCACCCCCCTAGATGTGTTGCTGGCCGCACAAATCT
TGCAGCAGCGTCTCGGACCAGCGCGTTAG

Upstream 100 bases:

>100_bases
TTTATGGCTTGTTGACCTCAAGCAGTACACTGGGATCCGGTTGAATGCGCTTTTGGATCCCATGCACCTTCTGATCCCGG
CGGCTGGCAGCGGCAAGCGA

Downstream 100 bases:

>100_bases
CTCAATTCAATCCCGGTAGGGCCAGTCTCAACCTTGGGAATTAAAAGTAGGAAGGAGGAAATCTAGGTTCACTAAGAGCA
CTTTGATCACCCCATCTCTT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MGAAVNKLLLPLLGQPILAWTLQAADQASSIEWIGILSQPGDWPAIEQILAAMDLSTPVQLLQGGATRQESVYRGVEYLY
NLGTVERVLIHDGARCLATPKLFDRCSAALQQVDGLIAGIPVKDTIKVAEAGPKGIQIQSTPERSRLWAAQTPQGFRLSM
LWQAHREALAQGWQVTDDAALFEKLGWPVYIVEGEESNLKLTTPLDVLLAAQILQQRLGPAR

Sequences:

>Translated_222_residues
MGAAVNKLLLPLLGQPILAWTLQAADQASSIEWIGILSQPGDWPAIEQILAAMDLSTPVQLLQGGATRQESVYRGVEYLY
NLGTVERVLIHDGARCLATPKLFDRCSAALQQVDGLIAGIPVKDTIKVAEAGPKGIQIQSTPERSRLWAAQTPQGFRLSM
LWQAHREALAQGWQVTDDAALFEKLGWPVYIVEGEESNLKLTTPLDVLLAAQILQQRLGPAR
>Mature_221_residues
GAAVNKLLLPLLGQPILAWTLQAADQASSIEWIGILSQPGDWPAIEQILAAMDLSTPVQLLQGGATRQESVYRGVEYLYN
LGTVERVLIHDGARCLATPKLFDRCSAALQQVDGLIAGIPVKDTIKVAEAGPKGIQIQSTPERSRLWAAQTPQGFRLSML
WQAHREALAQGWQVTDDAALFEKLGWPVYIVEGEESNLKLTTPLDVLLAAQILQQRLGPAR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=205, Percent_Identity=33.1707317073171, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 24196; Mature: 24065

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAAVNKLLLPLLGQPILAWTLQAADQASSIEWIGILSQPGDWPAIEQILAAMDLSTPVQ
CCHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHH
LLQGGATRQESVYRGVEYLYNLGTVERVLIHDGARCLATPKLFDRCSAALQQVDGLIAGI
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCC
PVKDTIKVAEAGPKGIQIQSTPERSRLWAAQTPQGFRLSMLWQAHREALAQGWQVTDDAA
CCHHHHHHHCCCCCCEEECCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
LFEKLGWPVYIVEGEESNLKLTTPLDVLLAAQILQQRLGPAR
HHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GAAVNKLLLPLLGQPILAWTLQAADQASSIEWIGILSQPGDWPAIEQILAAMDLSTPVQ
CHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHH
LLQGGATRQESVYRGVEYLYNLGTVERVLIHDGARCLATPKLFDRCSAALQQVDGLIAGI
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCC
PVKDTIKVAEAGPKGIQIQSTPERSRLWAAQTPQGFRLSMLWQAHREALAQGWQVTDDAA
CCHHHHHHHCCCCCCEEECCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
LFEKLGWPVYIVEGEESNLKLTTPLDVLLAAQILQQRLGPAR
HHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA