Click here to switch to the map view.

The map label for this gene is amt [C]

Identifier: 86610131

GI number: 86610131

Start: 2794011

End: 2795423

Strand: Direct

Name: amt [C]

Synonym: CYB_2705

Alternate gene names: 86610131

Gene position: 2794011-2795423 (Clockwise)

Preceding gene: 86610130

Following gene: 86610132

Centisome position: 91.71

GC content: 57.61

Gene sequence:

>1413_bases
ATGAGTACAACAATGATCCAAAAGCTCAAGAGCCAGGTGCCCCAGTGGCTCTGGCGACCGGCCAGGTGGAGTCTGACTGC
TGCTCTAATGGCTGCGCTGGTCTTTCTATTCGGCCAGGGAACTGCCTTTGCTCAAGAGGCCGAGGCAGTTCAGAAACACG
CTGACATTGTTTGGACGATCGTGGCAGCAGCCTTGGTGTTCTTCATGCAGGCAGGGTTCGCCATGGTGGAGACGGGCTTT
ACCCGCGCCAAGAATGCCGCCAACATCATGATGAAGAACTTGATGGACTTCTGCATGGGCTGTTGCGCCTTCTGGTTCAT
CGGGTTTGGCCTCATGTTCGGCAACAGTGGTGGCTTGTTCGGCACCGACTGGTTTGCTTTTGGCTGGCAGCAGGCTTACG
AGGCAGATGGCTGGCCCTTTACTTTCTTTGTCTTCCAGATGGTGTTTGCCGCCACTGCCGCCACCATTGTTTCCGGAGCC
ATGGCGGAGCGCACCAAGTTCTTGGCCTACTTGATCTACTCCATCGTTATTTCTGGGCTAATCTACCCCATCTCTGGGGG
TTGGGCCTGGAACGGGCTGTTCGGCGACTACAACGGCGGCACAGCCGGCTGGCTGGAAAATCTGGGCTACATTGATTTCG
CTGGATCGGGGGTGGTGCATTTGGTAGGCGGCGCTGCGGCCTTGGCAGGCGCCTTTGTGCTCGGCCCTCGCATTGGCAAG
TACGGCCCTAATGGAGAACCGCGGGCGATCCCTGGCCACAACCTGCCGCTGGGGATGTTGGGGGTGTTTATCCTCGCCTT
GGGCTGGATCGGGTTTAATGCCGGTTCCACCACTGCAGCCTCTGTCGATATTGGCTGGATTGCCATGAATACGCTGCTGG
CAGCTTCGGCTGGAGCAATTGGGGCGATGTTCACCTCTTGGGTTCATTTTGGCAAGCCGGATATGACCTTTGCCGCCAAC
GGTGTCCTGGCCGGCTTGGTGGGGATCACTGCCCCTTGCGATAGCGTCCATCCTTTGGGTGCTCTGATCATCGGCTCGGT
GGCCGGGATTCTGGTGGTGATTTCGGTGGTGATCCTGGATACGGTGCTGAAAATTGATGACCCGGTGGGCGCGATTTCGG
TACACGGCACCTGTGGTATCTGGGGGGTGTTGGCAGCAGGGATCCCCTTCTTTGCCAATGCCAACTATGCTGAAGAGGTC
ACCTGGGCCCAATTTGGGGTACAAGTGGTGGGCGCTCTGTTCTACTTCCTCTGGCCCTTTATTACCTGCTTGATCCTGTT
CTATATCCTCAAGGCTGTAACCGGCCTGCGCACCAGCGCCGAGGAAGAGATCGCCGGTTTGGATGTGGGCGAGCATGGCA
ACGTGGCCTACCCCGACTTCGTTACTGCTGCCACCGATTCGGAGCAATCTTGA

Upstream 100 bases:

>100_bases
CTGCATGTCTCTTTTGCTATATACCTGATGAGATGTTCTTGCCCCACTTTCCGGGTAATGTCTTTTGAGAAGTACTGTCC
CTTTAGGTGAGTCGAAAAGC

Downstream 100 bases:

>100_bases
GCCGTTGTGCTCGGTCTGACCTGGGGTTGCCGGATCGGAGCATTGCTGAACTGAGCATCAAATGAGCATCAAAAGGGAGG
GTCAGGGCTGGCCCTCTCTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSTTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTIVAAALVFFMQAGFAMVETGF
TRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGNSGGLFGTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGA
MAERTKFLAYLIYSIVISGLIYPISGGWAWNGLFGDYNGGTAGWLENLGYIDFAGSGVVHLVGGAAALAGAFVLGPRIGK
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAASVDIGWIAMNTLLAASAGAIGAMFTSWVHFGKPDMTFAAN
GVLAGLVGITAPCDSVHPLGALIIGSVAGILVVISVVILDTVLKIDDPVGAISVHGTCGIWGVLAAGIPFFANANYAEEV
TWAQFGVQVVGALFYFLWPFITCLILFYILKAVTGLRTSAEEEIAGLDVGEHGNVAYPDFVTAATDSEQS

Sequences:

>Translated_470_residues
MSTTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTIVAAALVFFMQAGFAMVETGF
TRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGNSGGLFGTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGA
MAERTKFLAYLIYSIVISGLIYPISGGWAWNGLFGDYNGGTAGWLENLGYIDFAGSGVVHLVGGAAALAGAFVLGPRIGK
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAASVDIGWIAMNTLLAASAGAIGAMFTSWVHFGKPDMTFAAN
GVLAGLVGITAPCDSVHPLGALIIGSVAGILVVISVVILDTVLKIDDPVGAISVHGTCGIWGVLAAGIPFFANANYAEEV
TWAQFGVQVVGALFYFLWPFITCLILFYILKAVTGLRTSAEEEIAGLDVGEHGNVAYPDFVTAATDSEQS
>Mature_469_residues
STTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTIVAAALVFFMQAGFAMVETGFT
RAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGNSGGLFGTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGAM
AERTKFLAYLIYSIVISGLIYPISGGWAWNGLFGDYNGGTAGWLENLGYIDFAGSGVVHLVGGAAALAGAFVLGPRIGKY
GPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAASVDIGWIAMNTLLAASAGAIGAMFTSWVHFGKPDMTFAANG
VLAGLVGITAPCDSVHPLGALIIGSVAGILVVISVVILDTVLKIDDPVGAISVHGTCGIWGVLAAGIPFFANANYAEEVT
WAQFGVQVVGALFYFLWPFITCLILFYILKAVTGLRTSAEEEIAGLDVGEHGNVAYPDFVTAATDSEQS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=34.3675417661098, Blast_Score=196, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17550180, Length=419, Percent_Identity=38.4248210023866, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17550182, Length=419, Percent_Identity=37.9474940334129, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI32564042, Length=454, Percent_Identity=32.3788546255507, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI133931100, Length=441, Percent_Identity=29.4784580498866, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=29.1375291375291, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6325396, Length=438, Percent_Identity=27.3972602739726, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=432, Percent_Identity=27.0833333333333, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078314, Length=417, Percent_Identity=37.1702637889688, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49754; Mature: 49623

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTI
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VAAALVFFMQAGFAMVETGFTRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGNSGGLF
HHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGAMAERTKFLAYLIYSIVISGL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYPISGGWAWNGLFGDYNGGTAGWLENLGYIDFAGSGVVHLVGGAAALAGAFVLGPRIGK
HEECCCCEEECCCCCCCCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAASVDIGWIAMNTLLAASAGAI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCHH
GAMFTSWVHFGKPDMTFAANGVLAGLVGITAPCDSVHPLGALIIGSVAGILVVISVVILD
HHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVLKIDDPVGAISVHGTCGIWGVLAAGIPFFANANYAEEVTWAQFGVQVVGALFYFLWPF
HHHHCCCCCCEEEECCCHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ITCLILFYILKAVTGLRTSAEEEIAGLDVGEHGNVAYPDFVTAATDSEQS
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
STTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTI
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VAAALVFFMQAGFAMVETGFTRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGNSGGLF
HHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGAMAERTKFLAYLIYSIVISGL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYPISGGWAWNGLFGDYNGGTAGWLENLGYIDFAGSGVVHLVGGAAALAGAFVLGPRIGK
HEECCCCEEECCCCCCCCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAASVDIGWIAMNTLLAASAGAI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCHH
GAMFTSWVHFGKPDMTFAANGVLAGLVGITAPCDSVHPLGALIIGSVAGILVVISVVILD
HHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVLKIDDPVGAISVHGTCGIWGVLAAGIPFFANANYAEEVTWAQFGVQVVGALFYFLWPF
HHHHCCCCCCEEEECCCHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ITCLILFYILKAVTGLRTSAEEEIAGLDVGEHGNVAYPDFVTAATDSEQS
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]