Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 86609687

GI number: 86609687

Start: 2350829

End: 2352202

Strand: Reverse

Name: nusA [H]

Synonym: CYB_2245

Alternate gene names: 86609687

Gene position: 2352202-2350829 (Counterclockwise)

Preceding gene: 86609688

Following gene: 86609686

Centisome position: 77.21

GC content: 59.32

Gene sequence:

>1374_bases
ATGGCGATGGTGCCTCTGCCGGGTTTGGCGGAAATGGTGGAGATGATCTCGCGGGAGCGCAACTTGCCCCGCCATGTGGT
CACCGATGCTCTGCGAGAGGCGCTGCTCAAGGGCTACGAACGCTACCGCAAAACCATCCAGCGAGATGTGGTCTTTGCAG
AAGACCACTTCCGCAATTTCGATGTGGAGTTGGATGTGGAGGCAGGAGGCTTTCGCGTTTTGGCCACCAAAGCCATCGTT
GAGCAGGTGCAAAACCCCGACCATGAGATCTCTCTGTACGATGTGCAGCAGGATTACCCAGAAGCCGAAGTAGGCTGGGA
AGTCACAGATGATGTCACCCCACAACAGGCGGAATTTGGCCGCATGGCGGCCATCCAAACCAAGCAGGTGCTGGCGCAAA
AGTTACGGGATCAGCAGCGCCGTCTGATTCAGGAAGAGTTCCGCGATCTGGAAAAAACCGTACTGCAGGCACGGGTGCTG
CGCACCGAGCGGCAGACGGTGATCATGTCGGTATCCAGCGGTTTCGGACAACCTGAGGTGGAAGCAGAACTGCCCAAGAA
GGAACAGCTGGCCAGCGATCGCTACACTCCCAACGCGGTGATGAAGGTCTATCTCAAGAAAGTCTATGAGGGATCCCGGC
GGGGGCCACAGCTTTTGGTTTCCCGGGCCGATGCTGGCTTGGTGGTGAACCTGTTTGCCAACGAGGTGCCAGAAATTGAA
GAGGATATCGTGCGGATTGTGGCAGTGGCGCGGGAGGCCAACCCCCCTTCCCGAAATGTGGGGCCACGTACCAAGATTGC
GGTCGATACGGTGGAGCGGGACGTGGATCCGGTGGGGGCCTGTATTGGCGCGAGGGGATCCCGGATTCAGGCGGTGGTAG
CAGAGCTGCGCAACGAGAAAATCGATGTCATCCGCTGGTCGCCGGATCCTGCCACTTACATTGCCAATGCCCTCAGCCCG
GCCAAGGTGGTGGAGGTGCGGCTGGTGGATCCGGAAATCCAGCAAGCTCATGTGTTGGTCAACAGCGACCAGCTCAGTTT
GGCCATTGGCAAAGAGGGGCAAAATGTGCGCCTGGCAGCCCGCCTCACCGGTTGGAAAATCGACATCAAGGAAGTGGAGA
GCTACCGCGCAGCGCTGGAGGAGGCCCAAGCGCAAGGGTTGCCCTATCCCTTCATCACTCCAGCGGCACTGGAGCGCTTC
CAGCGGCGGGCAGAGCGGGCTGCTTTGGGCCTAGAGGATGAGGAATACGAAGAAAAGCGCCGCCTGGAGGAAGAGGAGCT
GGCGGGGGACGATAGGCTGGAAGAAGACTGGGCTGACGAAGAGGAGGACTTCGACTACGACGCCGAGTTCGACATTGCCC
AGCCGAAGGGGTAA

Upstream 100 bases:

>100_bases
GGTGCGGCCCTCCCTTCGCATCAGAGATGCGGGTTCAGTTCCCGCTGTGGAGCAATAGCAACAGCAAGTTTCACGAAGTT
TGTGCAGGAGGACAGTGGTT

Downstream 100 bases:

>100_bases
TGATATACGCTCTACCCTCACTTTGCCCGTACACTTTGCTCGTAGAGGGCGGCTTCTGCCGGAAGTCTGGGCTTTGGAAT
GAGTTTTGCTGCAGGGGATC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAMVPLPGLAEMVEMISRERNLPRHVVTDALREALLKGYERYRKTIQRDVVFAEDHFRNFDVELDVEAGGFRVLATKAIV
EQVQNPDHEISLYDVQQDYPEAEVGWEVTDDVTPQQAEFGRMAAIQTKQVLAQKLRDQQRRLIQEEFRDLEKTVLQARVL
RTERQTVIMSVSSGFGQPEVEAELPKKEQLASDRYTPNAVMKVYLKKVYEGSRRGPQLLVSRADAGLVVNLFANEVPEIE
EDIVRIVAVAREANPPSRNVGPRTKIAVDTVERDVDPVGACIGARGSRIQAVVAELRNEKIDVIRWSPDPATYIANALSP
AKVVEVRLVDPEIQQAHVLVNSDQLSLAIGKEGQNVRLAARLTGWKIDIKEVESYRAALEEAQAQGLPYPFITPAALERF
QRRAERAALGLEDEEYEEKRRLEEEELAGDDRLEEDWADEEEDFDYDAEFDIAQPKG

Sequences:

>Translated_457_residues
MAMVPLPGLAEMVEMISRERNLPRHVVTDALREALLKGYERYRKTIQRDVVFAEDHFRNFDVELDVEAGGFRVLATKAIV
EQVQNPDHEISLYDVQQDYPEAEVGWEVTDDVTPQQAEFGRMAAIQTKQVLAQKLRDQQRRLIQEEFRDLEKTVLQARVL
RTERQTVIMSVSSGFGQPEVEAELPKKEQLASDRYTPNAVMKVYLKKVYEGSRRGPQLLVSRADAGLVVNLFANEVPEIE
EDIVRIVAVAREANPPSRNVGPRTKIAVDTVERDVDPVGACIGARGSRIQAVVAELRNEKIDVIRWSPDPATYIANALSP
AKVVEVRLVDPEIQQAHVLVNSDQLSLAIGKEGQNVRLAARLTGWKIDIKEVESYRAALEEAQAQGLPYPFITPAALERF
QRRAERAALGLEDEEYEEKRRLEEEELAGDDRLEEDWADEEEDFDYDAEFDIAQPKG
>Mature_456_residues
AMVPLPGLAEMVEMISRERNLPRHVVTDALREALLKGYERYRKTIQRDVVFAEDHFRNFDVELDVEAGGFRVLATKAIVE
QVQNPDHEISLYDVQQDYPEAEVGWEVTDDVTPQQAEFGRMAAIQTKQVLAQKLRDQQRRLIQEEFRDLEKTVLQARVLR
TERQTVIMSVSSGFGQPEVEAELPKKEQLASDRYTPNAVMKVYLKKVYEGSRRGPQLLVSRADAGLVVNLFANEVPEIEE
DIVRIVAVAREANPPSRNVGPRTKIAVDTVERDVDPVGACIGARGSRIQAVVAELRNEKIDVIRWSPDPATYIANALSPA
KVVEVRLVDPEIQQAHVLVNSDQLSLAIGKEGQNVRLAARLTGWKIDIKEVESYRAALEEAQAQGLPYPFITPAALERFQ
RRAERAALGLEDEEYEEKRRLEEEELAGDDRLEEDWADEEEDFDYDAEFDIAQPKG

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=367, Percent_Identity=31.6076294277929, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 51753; Mature: 51622

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMVPLPGLAEMVEMISRERNLPRHVVTDALREALLKGYERYRKTIQRDVVFAEDHFRNF
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHCCCC
DVELDVEAGGFRVLATKAIVEQVQNPDHEISLYDVQQDYPEAEVGWEVTDDVTPQQAEFG
CEEEEECCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEECCCCCCCHHHHH
RMAAIQTKQVLAQKLRDQQRRLIQEEFRDLEKTVLQARVLRTERQTVIMSVSSGFGQPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
EAELPKKEQLASDRYTPNAVMKVYLKKVYEGSRRGPQLLVSRADAGLVVNLFANEVPEIE
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEHHCCCCHHH
EDIVRIVAVAREANPPSRNVGPRTKIAVDTVERDVDPVGACIGARGSRIQAVVAELRNEK
HHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCC
IDVIRWSPDPATYIANALSPAKVVEVRLVDPEIQQAHVLVNSDQLSLAIGKEGQNVRLAA
EEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCCCEEEEE
RLTGWKIDIKEVESYRAALEEAQAQGLPYPFITPAALERFQRRAERAALGLEDEEYEEKR
EECCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
RLEEEELAGDDRLEEDWADEEEDFDYDAEFDIAQPKG
CCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMVPLPGLAEMVEMISRERNLPRHVVTDALREALLKGYERYRKTIQRDVVFAEDHFRNF
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHCCCC
DVELDVEAGGFRVLATKAIVEQVQNPDHEISLYDVQQDYPEAEVGWEVTDDVTPQQAEFG
CEEEEECCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEECCCCCCCHHHHH
RMAAIQTKQVLAQKLRDQQRRLIQEEFRDLEKTVLQARVLRTERQTVIMSVSSGFGQPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
EAELPKKEQLASDRYTPNAVMKVYLKKVYEGSRRGPQLLVSRADAGLVVNLFANEVPEIE
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEHHCCCCHHH
EDIVRIVAVAREANPPSRNVGPRTKIAVDTVERDVDPVGACIGARGSRIQAVVAELRNEK
HHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCC
IDVIRWSPDPATYIANALSPAKVVEVRLVDPEIQQAHVLVNSDQLSLAIGKEGQNVRLAA
EEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCCCEEEEE
RLTGWKIDIKEVESYRAALEEAQAQGLPYPFITPAALERFQRRAERAALGLEDEEYEEKR
EECCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
RLEEEELAGDDRLEEDWADEEEDFDYDAEFDIAQPKG
CCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]