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The map label for this gene is priA [H]

Identifier: 86609671

GI number: 86609671

Start: 2331655

End: 2334060

Strand: Direct

Name: priA [H]

Synonym: CYB_2227

Alternate gene names: 86609671

Gene position: 2331655-2334060 (Clockwise)

Preceding gene: 86609670

Following gene: 86609672

Centisome position: 76.53

GC content: 65.75

Gene sequence:

>2406_bases
GTGTGGGTGGATCATCCCCATTTGGGGGGTTGCTTTACCTATCGTGTGCCAGAGGGATCCGCGCCAGAAGCGGGCGACCT
GCTCAGCGTGCCCTTTGGTCGGCAAATGGCGGGGGCGGTGGCCTTGGGTTGGCGCTCCGAGCTGCCGGCAGGGGTGGATC
CGCAGTCGGTGCGGGAGATCGAGGCGGTGGTGGCCAAAGGGATCCTGCCCAAAGAATTCTGGCCCCTGCTGCAGCGGGTG
GCCGACTATTACCTCACCCCTCTGGCTCAGGTGGTGCGCACGGTATTGCCCCCCGGCTTGCTCAGCCGTTCTCAGCGGCG
TGTCCGCTTGCTCAGGCTCCCTGCCGAAGGGGAGGAATGTTCGCCGGCTGCCCAAACCCTGCTGCAGCTGCTTCAGGCCA
AAGGGGGAGAGCTGTCTTGGCGCTATGTGCAACAGCAGGTGCCCACTGCCGCCCTCGCCCTGCGGGAACTGCAGCGACGG
GGTTGGGTGGAGAGCTTTTGGCAGGAGCAACAGGGGGGCAGGGTGAGGACCCAACTGGCCGCCAGTCTGGCCGACGACTC
CCCAGAGGGGCTGAGCCTCCCTCAGCAGAAGGCGCTGCAGATCCTCAAGCAGAAGGGGGGAGAGCTGCTGCTCGCCGAGT
GGGAGCGCCTGGCAGGAGTGAGCCGAGCGGTTCTGCAGGGGTTGGCCCGCAAAGGCCGTGTCCACCTCTACGAGCGCCCA
CTGCTGCGGCTGGGAGATGCCAGCCTGCCGGGGTGGCGGGATCCGCCCAGGCCCTTGACGGCCGCCCAGGCCCAGGCGGT
AGAGGCGATCCGGCAAGCTTTGGGCCGGTTCCAGCGGTTTTTACTGCACGGGGTCACCGGTTCTGGGAAAACGGAAGTGT
ACCTGCAGGTGATCGCCCAGGTGCTGCAGCAAGGACGGTCGGCGCTGGTGCTGGTGCCCGAGATCGGCCTTACCCCGCAA
CTGATGGATCGGTTTCGGGCGCGGCTGCGATCCCAGGTGTGGGCCTACCACAGCGGCCTCTCGGAAGGCGAGCGCTACGA
CACCTGGCGGCAGATGCTGTCCTCCACCCCCCAAGTGGTGATCGGCACCCGCTCGGCGGTGTTTGCCCCCTTGTCCAAGC
TGGGCCTGATCGTCTTGGACGAAGAGCACGACGACAGCTTCAAGCAGGATCAGCCCCAGCCCTGCTACCACGCCCGTCAG
GTGGCCGAGTGGCGGGCCGAGCTGGCCGGTTGTCCCCTGGTCCTGGGCAGTGCAACCCCAGCGGTGGAAACCTATGCCGC
CCATCTGCAGGATCCGCAGCGGTGGATCCGGCTGGCCCTGCCGCAGCGCATCCCCCAAGCTGGGATCCCGGCCACCCTGC
CCCAACTGGAGCTGGTGGACATGCGCCAGGAGCTCCAAGCCGGCAACCGCAGCATCCTCAGCCGCCGTCTACACCAGGCC
CTGCAGCAGACGCTGGACAGAGGGGAACAGGCCATTCTCTTTGTCCCCCGCCGCGGCCACAGCACCTTCGTCTTGTGCCG
TAGCTGCGGGTTTGTCCTCACCTGCGAGCATTGTGATGTTTCTCTCACCTTTCACCTGGCCCATCTCGGCGGACAAGAAC
TGCGCTGCCACTACTGCGGCGCCCGCCAACCCCATCCGCAGCGCTGCCCCGCCTGTGGATCCCCCTACCTGAAGCACTTC
GGCACCGGCACCCAAAAAGTGGTGGAAGTGTTGCACCAGCATTGGCCGCAGCTCTCGATTTTGCGCTACGACAGCGACGC
CACCCGCCGCAAGGGATCCCATCGCGATTTGCTGGAGCAGTTTCGCAGCGGCAAGGCCCAGGTGCTGGTGGGCACGCAAA
TGCTCACCAAAGGGCTGGACATACCCCAGGTAACCTTGGTGGGCGTGATGGCCGCCGATGGGCTGTTGCACCAAGCCGAC
TATCGCGCCGGCGAGCGCAGCTTTCAACTGCTCACCCAGGTGGCAGGGCGCTCGGGCCGGGGATCCCTGCCAGGACAGGC
GATTATCCAGACCTATCTGCCGGAGCACCCGATTTTGAGGGCGGTACTGGCCCAAAATTACGAGGGCTTCTTGCAGGCAG
AACTGCAACAACGGCGAGAGGGGGGCTATCCTCCCCACCGAGCTTTGATTTTGATCCGCTTGAGCAGCACCCGCCTGGAA
GCCCTGGAGCGTTATTGCCATCGGGTGGCCGAACGCCTACAGGGGATCCCAGCAGAGGTGTTGGGGCCGGGGCCGGCCCA
GGTGGAGCGGGTTGCCGGACGCTACCGCTGGCAGATCCTGTTGAAGCAGCTTCCCGACCAAGGTTGGGATCGCCCGAAGC
TGGCTGCGCAGATCCAGGCCGCCATTGGCCATCCCCCCCAAGGGATCCGCGTCAGCATAGATGTGGATCCCCTGCGGATT
CTCTAG

Upstream 100 bases:

>100_bases
TACCGTCTCGGGCAAGCAAGCGGGCGGTCGATCTGCCGAGGTTTCTGCCCCGTCCGTGAGGGATCCCGTGTTGCAGCCTG
TTCCTCCCCGCTGGCTGGAA

Downstream 100 bases:

>100_bases
GAACAGTTGACCCGGCGCGCGGCAAGCCCGCCGATCCCGCGAGATCTTCCCGCGAGATCTTAAGGTAGTCTGGTTTCGGG
GCCACCCGTATCCAACTCAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MWVDHPHLGGCFTYRVPEGSAPEAGDLLSVPFGRQMAGAVALGWRSELPAGVDPQSVREIEAVVAKGILPKEFWPLLQRV
ADYYLTPLAQVVRTVLPPGLLSRSQRRVRLLRLPAEGEECSPAAQTLLQLLQAKGGELSWRYVQQQVPTAALALRELQRR
GWVESFWQEQQGGRVRTQLAASLADDSPEGLSLPQQKALQILKQKGGELLLAEWERLAGVSRAVLQGLARKGRVHLYERP
LLRLGDASLPGWRDPPRPLTAAQAQAVEAIRQALGRFQRFLLHGVTGSGKTEVYLQVIAQVLQQGRSALVLVPEIGLTPQ
LMDRFRARLRSQVWAYHSGLSEGERYDTWRQMLSSTPQVVIGTRSAVFAPLSKLGLIVLDEEHDDSFKQDQPQPCYHARQ
VAEWRAELAGCPLVLGSATPAVETYAAHLQDPQRWIRLALPQRIPQAGIPATLPQLELVDMRQELQAGNRSILSRRLHQA
LQQTLDRGEQAILFVPRRGHSTFVLCRSCGFVLTCEHCDVSLTFHLAHLGGQELRCHYCGARQPHPQRCPACGSPYLKHF
GTGTQKVVEVLHQHWPQLSILRYDSDATRRKGSHRDLLEQFRSGKAQVLVGTQMLTKGLDIPQVTLVGVMAADGLLHQAD
YRAGERSFQLLTQVAGRSGRGSLPGQAIIQTYLPEHPILRAVLAQNYEGFLQAELQQRREGGYPPHRALILIRLSSTRLE
ALERYCHRVAERLQGIPAEVLGPGPAQVERVAGRYRWQILLKQLPDQGWDRPKLAAQIQAAIGHPPQGIRVSIDVDPLRI
L

Sequences:

>Translated_801_residues
MWVDHPHLGGCFTYRVPEGSAPEAGDLLSVPFGRQMAGAVALGWRSELPAGVDPQSVREIEAVVAKGILPKEFWPLLQRV
ADYYLTPLAQVVRTVLPPGLLSRSQRRVRLLRLPAEGEECSPAAQTLLQLLQAKGGELSWRYVQQQVPTAALALRELQRR
GWVESFWQEQQGGRVRTQLAASLADDSPEGLSLPQQKALQILKQKGGELLLAEWERLAGVSRAVLQGLARKGRVHLYERP
LLRLGDASLPGWRDPPRPLTAAQAQAVEAIRQALGRFQRFLLHGVTGSGKTEVYLQVIAQVLQQGRSALVLVPEIGLTPQ
LMDRFRARLRSQVWAYHSGLSEGERYDTWRQMLSSTPQVVIGTRSAVFAPLSKLGLIVLDEEHDDSFKQDQPQPCYHARQ
VAEWRAELAGCPLVLGSATPAVETYAAHLQDPQRWIRLALPQRIPQAGIPATLPQLELVDMRQELQAGNRSILSRRLHQA
LQQTLDRGEQAILFVPRRGHSTFVLCRSCGFVLTCEHCDVSLTFHLAHLGGQELRCHYCGARQPHPQRCPACGSPYLKHF
GTGTQKVVEVLHQHWPQLSILRYDSDATRRKGSHRDLLEQFRSGKAQVLVGTQMLTKGLDIPQVTLVGVMAADGLLHQAD
YRAGERSFQLLTQVAGRSGRGSLPGQAIIQTYLPEHPILRAVLAQNYEGFLQAELQQRREGGYPPHRALILIRLSSTRLE
ALERYCHRVAERLQGIPAEVLGPGPAQVERVAGRYRWQILLKQLPDQGWDRPKLAAQIQAAIGHPPQGIRVSIDVDPLRI
L
>Mature_801_residues
MWVDHPHLGGCFTYRVPEGSAPEAGDLLSVPFGRQMAGAVALGWRSELPAGVDPQSVREIEAVVAKGILPKEFWPLLQRV
ADYYLTPLAQVVRTVLPPGLLSRSQRRVRLLRLPAEGEECSPAAQTLLQLLQAKGGELSWRYVQQQVPTAALALRELQRR
GWVESFWQEQQGGRVRTQLAASLADDSPEGLSLPQQKALQILKQKGGELLLAEWERLAGVSRAVLQGLARKGRVHLYERP
LLRLGDASLPGWRDPPRPLTAAQAQAVEAIRQALGRFQRFLLHGVTGSGKTEVYLQVIAQVLQQGRSALVLVPEIGLTPQ
LMDRFRARLRSQVWAYHSGLSEGERYDTWRQMLSSTPQVVIGTRSAVFAPLSKLGLIVLDEEHDDSFKQDQPQPCYHARQ
VAEWRAELAGCPLVLGSATPAVETYAAHLQDPQRWIRLALPQRIPQAGIPATLPQLELVDMRQELQAGNRSILSRRLHQA
LQQTLDRGEQAILFVPRRGHSTFVLCRSCGFVLTCEHCDVSLTFHLAHLGGQELRCHYCGARQPHPQRCPACGSPYLKHF
GTGTQKVVEVLHQHWPQLSILRYDSDATRRKGSHRDLLEQFRSGKAQVLVGTQMLTKGLDIPQVTLVGVMAADGLLHQAD
YRAGERSFQLLTQVAGRSGRGSLPGQAIIQTYLPEHPILRAVLAQNYEGFLQAELQQRREGGYPPHRALILIRLSSTRLE
ALERYCHRVAERLQGIPAEVLGPGPAQVERVAGRYRWQILLKQLPDQGWDRPKLAAQIQAAIGHPPQGIRVSIDVDPLRI
L

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=550, Percent_Identity=41.8181818181818, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 89194; Mature: 89194

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVDHPHLGGCFTYRVPEGSAPEAGDLLSVPFGRQMAGAVALGWRSELPAGVDPQSVREI
CCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHCCCHHCCCCCCCHHHHHHH
EAVVAKGILPKEFWPLLQRVADYYLTPLAQVVRTVLPPGLLSRSQRRVRLLRLPAEGEEC
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCCC
SPAAQTLLQLLQAKGGELSWRYVQQQVPTAALALRELQRRGWVESFWQEQQGGRVRTQLA
CHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHH
ASLADDSPEGLSLPQQKALQILKQKGGELLLAEWERLAGVSRAVLQGLARKGRVHLYERP
HHHCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LLRLGDASLPGWRDPPRPLTAAQAQAVEAIRQALGRFQRFLLHGVTGSGKTEVYLQVIAQ
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VLQQGRSALVLVPEIGLTPQLMDRFRARLRSQVWAYHSGLSEGERYDTWRQMLSSTPQVV
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEE
IGTRSAVFAPLSKLGLIVLDEEHDDSFKQDQPQPCYHARQVAEWRAELAGCPLVLGSATP
EECCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCC
AVETYAAHLQDPQRWIRLALPQRIPQAGIPATLPQLELVDMRQELQAGNRSILSRRLHQA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LQQTLDRGEQAILFVPRRGHSTFVLCRSCGFVLTCEHCDVSLTFHLAHLGGQELRCHYCG
HHHHHCCCCEEEEEEECCCCCEEEEEECCCCEEEECCCCEEEEEEEECCCCCCEEEEECC
ARQPHPQRCPACGSPYLKHFGTGTQKVVEVLHQHWPQLSILRYDSDATRRKGSHRDLLEQ
CCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHHCCCCHHHHHHH
FRSGKAQVLVGTQMLTKGLDIPQVTLVGVMAADGLLHQADYRAGERSFQLLTQVAGRSGR
HHCCCCEEEECHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCC
GSLPGQAIIQTYLPEHPILRAVLAQNYEGFLQAELQQRREGGYPPHRALILIRLSSTRLE
CCCCHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHH
ALERYCHRVAERLQGIPAEVLGPGPAQVERVAGRYRWQILLKQLPDQGWDRPKLAAQIQA
HHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AIGHPPQGIRVSIDVDPLRIL
HHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MWVDHPHLGGCFTYRVPEGSAPEAGDLLSVPFGRQMAGAVALGWRSELPAGVDPQSVREI
CCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHCCCHHCCCCCCCHHHHHHH
EAVVAKGILPKEFWPLLQRVADYYLTPLAQVVRTVLPPGLLSRSQRRVRLLRLPAEGEEC
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCCC
SPAAQTLLQLLQAKGGELSWRYVQQQVPTAALALRELQRRGWVESFWQEQQGGRVRTQLA
CHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHH
ASLADDSPEGLSLPQQKALQILKQKGGELLLAEWERLAGVSRAVLQGLARKGRVHLYERP
HHHCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LLRLGDASLPGWRDPPRPLTAAQAQAVEAIRQALGRFQRFLLHGVTGSGKTEVYLQVIAQ
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VLQQGRSALVLVPEIGLTPQLMDRFRARLRSQVWAYHSGLSEGERYDTWRQMLSSTPQVV
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEE
IGTRSAVFAPLSKLGLIVLDEEHDDSFKQDQPQPCYHARQVAEWRAELAGCPLVLGSATP
EECCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCC
AVETYAAHLQDPQRWIRLALPQRIPQAGIPATLPQLELVDMRQELQAGNRSILSRRLHQA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LQQTLDRGEQAILFVPRRGHSTFVLCRSCGFVLTCEHCDVSLTFHLAHLGGQELRCHYCG
HHHHHCCCCEEEEEEECCCCCEEEEEECCCCEEEECCCCEEEEEEEECCCCCCEEEEECC
ARQPHPQRCPACGSPYLKHFGTGTQKVVEVLHQHWPQLSILRYDSDATRRKGSHRDLLEQ
CCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHHCCCCHHHHHHH
FRSGKAQVLVGTQMLTKGLDIPQVTLVGVMAADGLLHQADYRAGERSFQLLTQVAGRSGR
HHCCCCEEEECHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCC
GSLPGQAIIQTYLPEHPILRAVLAQNYEGFLQAELQQRREGGYPPHRALILIRLSSTRLE
CCCCHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHH
ALERYCHRVAERLQGIPAEVLGPGPAQVERVAGRYRWQILLKQLPDQGWDRPKLAAQIQA
HHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AIGHPPQGIRVSIDVDPLRIL
HHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]