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The map label for this gene is alsS [H]

Identifier: 86609591

GI number: 86609591

Start: 2243363

End: 2245000

Strand: Reverse

Name: alsS [H]

Synonym: CYB_2144

Alternate gene names: 86609591

Gene position: 2245000-2243363 (Counterclockwise)

Preceding gene: 86609598

Following gene: 86609590

Centisome position: 73.69

GC content: 60.13

Gene sequence:

>1638_bases
ATGAATACCGCCGAACTGCTGGTTCGCTGCCTGGAAAATGAGGGGGTGCAGTACATCTTCGGCGTTCCTGGCGAAGAAAA
CCTGGAAGTCCTGCAAGCCCTGCGCCGCTCCTCAATTCGCTTTATCACCACCCGCCACGAACAGGGGGCCGCCTTCATGG
CCGATGTCTATGGCCGGCTGACGGGCAGAGCGGGGGTGTGTCTCTCCACCTTGGGGCCGGGCGCCACCAACCTGATGACC
GGAGTTGCCGACGCCAACCTGGATGGAGCACCCCTAGTGGCCATCACCGGCCAAGTGGGCACCGACCGCATGCACATCGA
ATCCCATCAATACCTGGATTTGGTGGCCATGTTTGCCCCCGTCACCAAGTGGAATGCCCAGATCGTGCGCCCCAGCATCA
CCCCGGAGATCGTGCGCAAGGCTTTCAAATTGGCCCAAACCGAGAAGCCCGGGGCCGTCCACATCGACTTGCCGGAAAAC
ATCGCCGCCATGGAAGCCAGCGGCACTCCCTTGAGGAGAGATCGCCTGGACAAAACCTACGCCTCCTATCAAAGCCTGAA
CGAAGCGGCGTCCCTGATCTCCCGCGCCCGCAACCCCTTGATTTTGGCCGGCAATGGGGCCATTCGCGCCCAAGCCAGCA
GCGCCCTCACGGAATTTGCCACCCGCTTGAACATCCCCGTGGTCAACACCTTCATGGGCAAAGGAGTGATCCCCTACACC
CATCCCCTGTCCCTGTGGGCCGTTGGCCTGCAACAGCGGGATCTGATCAGTTGTGGCTTCGACCGCACCGATCTGCTGAT
CGCCGTCGGCTACGACCTCATCGAGTACTCTCCCAAGAAATGGAACCCCGACGGCACCATCCCCATCGTTCACATCGCTG
CCCTGCCCGCAGAAATCGACAGCAGCTACATTCCGCAAGTGGAGGTGGTGGGAGACATTTCCGACTCCCTGATGGAGATC
CTGCGCCGCTGCGATCGCACGGGCAAGCCGGATCCCTATGGCCTCAGCCTGCGGCAGAACATCGTCCGCGAGTACACCTG
CTATGCCCACGATCAGAGCTACCCGATCAAGCCCCAAAAGCTGATCTACGACCTGCGCCAAGTCCTGGAACCCGAAGACA
TCGTCATCTCCGATGTCGGCGCCCACAAAATGTGGATGGCCCGCCTCTACCACTGTGACAAACCCAACACCTGTCTCATC
TCCAACGGCTTTGCCGCCATGGGCATCGCCATTCCGGGGGCCTTGGCCGCCAAATTGGTGCATCCCGACCGGCGCATCGT
GGCCGTAACCGGAGATGGAGGGTTCATGATGAACTGCCAAGAGCTGGAAACTGCCCTCAGGGTGGGCACTCCCTTTGTAA
CTCTCATCTTCAACGATGGCGGCTATGGGCTGATCGAGTGGAAGCAACACAATCAATACAACGAGGCTGCTTTCGTCAAG
TTCGGCAATCCAGACTTCGTTAAGCTGGCGGAGAGCATGGGCCTCAAGGGCTACCGTGTCGAAAAGGCTGCCGAGCTGAT
CCCCATCCTGAAGCAGGCCCTCGAGCAGCCGGTGCCCACCCTCATCGACTGCCCTGTCGATTACAGCGAAAACCTTTACC
TAACCCAGCGGTCGCAAGCGTTGGTCTGCGAGGCTTAG

Upstream 100 bases:

>100_bases
AGCCTGCCCCCGTCCCCAAGCCCAGAAAACAAGTTATCCCTTGCCCCGTCCGCCGTCTATGGTGGGATCCAGATCCACCG
TTCTCCATACCCTTGCCACC

Downstream 100 bases:

>100_bases
GGGACGAGATGGATGTGAATGCGAGATTTGAGCCGAGTTGGGGCATAGTAAGCCCAGGGTTGGCGGCCTCTGGGCAGTCC
AGACTTATGTATCTTTACCG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNTAELLVRCLENEGVQYIFGVPGEENLEVLQALRRSSIRFITTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFAPVTKWNAQIVRPSITPEIVRKAFKLAQTEKPGAVHIDLPEN
IAAMEASGTPLRRDRLDKTYASYQSLNEAASLISRARNPLILAGNGAIRAQASSALTEFATRLNIPVVNTFMGKGVIPYT
HPLSLWAVGLQQRDLISCGFDRTDLLIAVGYDLIEYSPKKWNPDGTIPIVHIAALPAEIDSSYIPQVEVVGDISDSLMEI
LRRCDRTGKPDPYGLSLRQNIVREYTCYAHDQSYPIKPQKLIYDLRQVLEPEDIVISDVGAHKMWMARLYHCDKPNTCLI
SNGFAAMGIAIPGALAAKLVHPDRRIVAVTGDGGFMMNCQELETALRVGTPFVTLIFNDGGYGLIEWKQHNQYNEAAFVK
FGNPDFVKLAESMGLKGYRVEKAAELIPILKQALEQPVPTLIDCPVDYSENLYLTQRSQALVCEA

Sequences:

>Translated_545_residues
MNTAELLVRCLENEGVQYIFGVPGEENLEVLQALRRSSIRFITTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFAPVTKWNAQIVRPSITPEIVRKAFKLAQTEKPGAVHIDLPEN
IAAMEASGTPLRRDRLDKTYASYQSLNEAASLISRARNPLILAGNGAIRAQASSALTEFATRLNIPVVNTFMGKGVIPYT
HPLSLWAVGLQQRDLISCGFDRTDLLIAVGYDLIEYSPKKWNPDGTIPIVHIAALPAEIDSSYIPQVEVVGDISDSLMEI
LRRCDRTGKPDPYGLSLRQNIVREYTCYAHDQSYPIKPQKLIYDLRQVLEPEDIVISDVGAHKMWMARLYHCDKPNTCLI
SNGFAAMGIAIPGALAAKLVHPDRRIVAVTGDGGFMMNCQELETALRVGTPFVTLIFNDGGYGLIEWKQHNQYNEAAFVK
FGNPDFVKLAESMGLKGYRVEKAAELIPILKQALEQPVPTLIDCPVDYSENLYLTQRSQALVCEA
>Mature_545_residues
MNTAELLVRCLENEGVQYIFGVPGEENLEVLQALRRSSIRFITTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFAPVTKWNAQIVRPSITPEIVRKAFKLAQTEKPGAVHIDLPEN
IAAMEASGTPLRRDRLDKTYASYQSLNEAASLISRARNPLILAGNGAIRAQASSALTEFATRLNIPVVNTFMGKGVIPYT
HPLSLWAVGLQQRDLISCGFDRTDLLIAVGYDLIEYSPKKWNPDGTIPIVHIAALPAEIDSSYIPQVEVVGDISDSLMEI
LRRCDRTGKPDPYGLSLRQNIVREYTCYAHDQSYPIKPQKLIYDLRQVLEPEDIVISDVGAHKMWMARLYHCDKPNTCLI
SNGFAAMGIAIPGALAAKLVHPDRRIVAVTGDGGFMMNCQELETALRVGTPFVTLIFNDGGYGLIEWKQHNQYNEAAFVK
FGNPDFVKLAESMGLKGYRVEKAAELIPILKQALEQPVPTLIDCPVDYSENLYLTQRSQALVCEA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=553, Percent_Identity=26.5822784810127, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=25.045045045045, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790104, Length=538, Percent_Identity=31.4126394052045, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=33.2737030411449, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1786717, Length=568, Percent_Identity=28.3450704225352, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=25.9259259259259, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788716, Length=544, Percent_Identity=27.5735294117647, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=481, Percent_Identity=27.6507276507277, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=481, Percent_Identity=27.6507276507277, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=551, Percent_Identity=23.4119782214156, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=31.5978456014363, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6320816, Length=537, Percent_Identity=25.1396648044693, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323163, Length=512, Percent_Identity=23.046875, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=525, Percent_Identity=22.0952380952381, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=527, Percent_Identity=23.5294117647059, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=26.654740608229, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59942; Mature: 59942

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAELLVRCLENEGVQYIFGVPGEENLEVLQALRRSSIRFITTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHH
TGRAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFAP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHC
VTKWNAQIVRPSITPEIVRKAFKLAQTEKPGAVHIDLPENIAAMEASGTPLRRDRLDKTY
CCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHH
ASYQSLNEAASLISRARNPLILAGNGAIRAQASSALTEFATRLNIPVVNTFMGKGVIPYT
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLSLWAVGLQQRDLISCGFDRTDLLIAVGYDLIEYSPKKWNPDGTIPIVHIAALPAEID
CCHHHHHCCCCCCCHHHCCCCCCCEEEEECCHHHHCCCCCCCCCCCEEEEEEEECCHHCC
SSYIPQVEVVGDISDSLMEILRRCDRTGKPDPYGLSLRQNIVREYTCYAHDQSYPIKPQK
CCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCHHH
LIYDLRQVLEPEDIVISDVGAHKMWMARLYHCDKPNTCLISNGFAAMGIAIPGALAAKLV
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEECCCEEEHHHCCHHHHHHHC
HPDRRIVAVTGDGGFMMNCQELETALRVGTPFVTLIFNDGGYGLIEWKQHNQYNEAAFVK
CCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEE
FGNPDFVKLAESMGLKGYRVEKAAELIPILKQALEQPVPTLIDCPVDYSENLYLTQRSQA
ECCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCCE
LVCEA
EEECC
>Mature Secondary Structure
MNTAELLVRCLENEGVQYIFGVPGEENLEVLQALRRSSIRFITTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHH
TGRAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFAP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHC
VTKWNAQIVRPSITPEIVRKAFKLAQTEKPGAVHIDLPENIAAMEASGTPLRRDRLDKTY
CCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHH
ASYQSLNEAASLISRARNPLILAGNGAIRAQASSALTEFATRLNIPVVNTFMGKGVIPYT
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLSLWAVGLQQRDLISCGFDRTDLLIAVGYDLIEYSPKKWNPDGTIPIVHIAALPAEID
CCHHHHHCCCCCCCHHHCCCCCCCEEEEECCHHHHCCCCCCCCCCCEEEEEEEECCHHCC
SSYIPQVEVVGDISDSLMEILRRCDRTGKPDPYGLSLRQNIVREYTCYAHDQSYPIKPQK
CCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCHHH
LIYDLRQVLEPEDIVISDVGAHKMWMARLYHCDKPNTCLISNGFAAMGIAIPGALAAKLV
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEECCCEEEHHHCCHHHHHHHC
HPDRRIVAVTGDGGFMMNCQELETALRVGTPFVTLIFNDGGYGLIEWKQHNQYNEAAFVK
CCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEE
FGNPDFVKLAESMGLKGYRVEKAAELIPILKQALEQPVPTLIDCPVDYSENLYLTQRSQA
ECCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCCE
LVCEA
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]