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The map label for this gene is nth [H]

Identifier: 86609598

GI number: 86609598

Start: 2252545

End: 2253183

Strand: Reverse

Name: nth [H]

Synonym: CYB_2151

Alternate gene names: 86609598

Gene position: 2253183-2252545 (Counterclockwise)

Preceding gene: 86609599

Following gene: 86609591

Centisome position: 73.96

GC content: 62.91

Gene sequence:

>639_bases
ATGCGGCTAGAGCAGTGCTTCGGGATCCCCCAGCGGGCTCCCAATGGCCTTTCCCCTCACCCCGATCTCCTGGACGAGTT
GGTGGGCACGATCCTTTCCCAGAACACCACCGATGCCAACAGCAGCCGTGCCTTCCGCGCCCTCAAAGCCGCCTTTCCCT
CTTGGGAAGCGGTCTTGGCTGCCGACCCGGCAGATCTGGCCCAGGTGATCCGCTCCGGCGGCTTAGCCCACCTTAAAGCA
GCCCGCATTCAGGAGATCTTGGCCGCCATTCAAGAACGACAGGGATCCCTGAGCCTCGACTTTCTGCGCGACCTGGACGA
CGCCGGCGCCCTCGACTTTCTGCTCTCGTTAAAAGGGGTAGGGCTGAAGACCGCCACCTGTGTGTTGTTGTTCGGGCTGG
GTCGGGATCTCTGCCCGGTGGATACCCATGTGCATCGAGTGGCCAACCGTCTGGGCTTGGTGCGGGCCAAACATCCCGAC
GACACTTTTGCTCAGCTTTCCCCTCAGATCCCCAGAGGCAAAGCCTATTCCCTGCACGTCAACTTTGTCCGGCTGGGCAA
GCGCATCTGCAAGGCCCGTATGCCCGAATGTGGCCGCTGCCCGCTGCGTCCCACCTGCCCTTCTGGGCGGCAGCGCTGA

Upstream 100 bases:

>100_bases
GGACAGCCTTTCCCAACGCCCGCCTGTGCTCGCCAAAGCGGCGGAGTCCCCTGCCCTGCTGGCAGAACTCCCGCCCGCTG
ACAGGATGGAATCGCTGTTG

Downstream 100 bases:

>100_bases
TCCCGACAGCAGGGCTCTATTCTGCGGGCGCAGTAGAAGCATCGGGGATCCCCTCTTGTCCGTTCCTGCCGGCGTCTCTC
TCTGCTGGGGGAACTTCCAA

Product: base excision DNA repair protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRLEQCFGIPQRAPNGLSPHPDLLDELVGTILSQNTTDANSSRAFRALKAAFPSWEAVLAADPADLAQVIRSGGLAHLKA
ARIQEILAAIQERQGSLSLDFLRDLDDAGALDFLLSLKGVGLKTATCVLLFGLGRDLCPVDTHVHRVANRLGLVRAKHPD
DTFAQLSPQIPRGKAYSLHVNFVRLGKRICKARMPECGRCPLRPTCPSGRQR

Sequences:

>Translated_212_residues
MRLEQCFGIPQRAPNGLSPHPDLLDELVGTILSQNTTDANSSRAFRALKAAFPSWEAVLAADPADLAQVIRSGGLAHLKA
ARIQEILAAIQERQGSLSLDFLRDLDDAGALDFLLSLKGVGLKTATCVLLFGLGRDLCPVDTHVHRVANRLGLVRAKHPD
DTFAQLSPQIPRGKAYSLHVNFVRLGKRICKARMPECGRCPLRPTCPSGRQR
>Mature_212_residues
MRLEQCFGIPQRAPNGLSPHPDLLDELVGTILSQNTTDANSSRAFRALKAAFPSWEAVLAADPADLAQVIRSGGLAHLKA
ARIQEILAAIQERQGSLSLDFLRDLDDAGALDFLLSLKGVGLKTATCVLLFGLGRDLCPVDTHVHRVANRLGLVRAKHPD
DTFAQLSPQIPRGKAYSLHVNFVRLGKRICKARMPECGRCPLRPTCPSGRQR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=187, Percent_Identity=28.8770053475936, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787920, Length=196, Percent_Identity=27.0408163265306, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=187, Percent_Identity=31.5508021390374, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23075; Mature: 23075

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEQCFGIPQRAPNGLSPHPDLLDELVGTILSQNTTDANSSRAFRALKAAFPSWEAVLA
CCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEE
ADPADLAQVIRSGGLAHLKAARIQEILAAIQERQGSLSLDFLRDLDDAGALDFLLSLKGV
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCC
GLKTATCVLLFGLGRDLCPVDTHVHRVANRLGLVRAKHPDDTFAQLSPQIPRGKAYSLHV
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEH
NFVRLGKRICKARMPECGRCPLRPTCPSGRQR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLEQCFGIPQRAPNGLSPHPDLLDELVGTILSQNTTDANSSRAFRALKAAFPSWEAVLA
CCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEE
ADPADLAQVIRSGGLAHLKAARIQEILAAIQERQGSLSLDFLRDLDDAGALDFLLSLKGV
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCC
GLKTATCVLLFGLGRDLCPVDTHVHRVANRLGLVRAKHPDDTFAQLSPQIPRGKAYSLHV
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEH
NFVRLGKRICKARMPECGRCPLRPTCPSGRQR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]