BacMap

Definition	Synechococcus sp. JA-2-3B'a(2-13), complete genome.
Accession	NC_007776
Length	3,046,682

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The map label for this gene is miaA

Identifier: 86609493

GI number: 86609493

Start: 2146323

End: 2147387

Strand: Reverse

Name: miaA

Synonym: CYB_2044

Alternate gene names: 86609493

Gene position: 2147387-2146323 (Counterclockwise)

Preceding gene: 86609495

Following gene: 86609490

Centisome position: 70.48

GC content: 62.63

Gene sequence:

>1065_bases
ATGCGCATTACCCTCGAGAATGGCATTCCCGCTGCAGCAGCTTCTGACCGGACAGAATGGCCGGGATTGATCGTCATTGC
CGGCCCCACGGCCACCGGGAAATCTCGACAGGCACTGCTTTTGGCCCAACGGCTGGGATCCCCGTTGTTGAATGCGGACT
CTCGCCAGGTCTACCGCGAGTTCGACATCGGCACAGCCAAGCCTACCCCCGCCGAGCAGGCCCTTTGGCCCCACGAGTTG
ATCGACATTGTTGATCCCTGCCACACCTACACGGTGGCTGAGTTTCAGCAGGCGGCCCAAGCCCGCATCGCCGAGGCCCA
TCGGCAGGGACAGACTCCCATCTTGGTGGGGGGCACCGGTCTCTACATCCAATCGGTGACTGCTGGCCTAGGGATCCCTG
CTGTACCCCCGCAGCCCCAACTGAGAAGACAACTGGAAACTTGGCCCCCTGAGATCCGCTACGCCTGGCTGCAGCAGTTG
GATCCCGTTGCCGCCCAGCACATTCATCCCCATGACGCGGTGCGCACGCTGCGGGCTTTGGAGATCGTTTACACCACCGG
GAAGCCGGCTTCCAGCCTAAGGCGGGCTCATCCCCCCCATTACCCCATCTTGATCCTGGGGCTGCGCTGCCCAATGCCCC
GTTTGGAACAACGCATTGCCCGGCGCACGGCGGAGATGATGGAGCGGGGGTGGATCGAGGAGGTGAAAACCCTGCGGGAG
CGCTACGGGCCAGACTTACCCCTCTTGCAGACCTTGGGCTATGCGGAGATCGGCGCTTACCTGGAGGGGCGGATCCCGGA
AGCCGAACTGCAACCCCTGATTGTGCGGCGCACCCGCCAATTTGCCAAGCGGCAGATGACTTGGTTTCGAGCCATGCTGG
GGATCCAAAAGGGCCATGCCTACGGCACCCTGTCGGGAAGGGGTCGATGCGTGGCCCTGTGGCTGGACTGCGAAGCTGAG
GATTTGCCGGAGCAGATCTGGAAGCAGGTCAAGGCTTGGATGGCAGCTCCGACCACTGTTGAAACCAGTCCCGCCGCCGC
TGAGCATCGGCTTTTCGATCCGTAA

Upstream 100 bases:

>100_bases
ACCGATCCTAACCCAAAGAGACAAGGTTCTTCGAGAATCTTAATCTGGTCTCTGAGGAAAGTTGCTTGCCAGTCTGCCCT
GCCGATGGTTTGGGTAGCGA

Downstream 100 bases:

>100_bases
GGATCTCCAACACCCGCACCCCTTGGGCAGGCAAGGGATCCAAAGCTTGGGCCAGCTCCTGCCAGCTTTCCACCCGCCGG
TGGGGAATGCCGTGGGCTTG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRITLENGIPAAAASDRTEWPGLIVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYREFDIGTAKPTPAEQALWPHEL
IDIVDPCHTYTVAEFQQAAQARIAEAHRQGQTPILVGGTGLYIQSVTAGLGIPAVPPQPQLRRQLETWPPEIRYAWLQQL
DPVAAQHIHPHDAVRTLRALEIVYTTGKPASSLRRAHPPHYPILILGLRCPMPRLEQRIARRTAEMMERGWIEEVKTLRE
RYGPDLPLLQTLGYAEIGAYLEGRIPEAELQPLIVRRTRQFAKRQMTWFRAMLGIQKGHAYGTLSGRGRCVALWLDCEAE
DLPEQIWKQVKAWMAAPTTVETSPAAAEHRLFDP

Sequences:

>Translated_354_residues
MRITLENGIPAAAASDRTEWPGLIVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYREFDIGTAKPTPAEQALWPHEL
IDIVDPCHTYTVAEFQQAAQARIAEAHRQGQTPILVGGTGLYIQSVTAGLGIPAVPPQPQLRRQLETWPPEIRYAWLQQL
DPVAAQHIHPHDAVRTLRALEIVYTTGKPASSLRRAHPPHYPILILGLRCPMPRLEQRIARRTAEMMERGWIEEVKTLRE
RYGPDLPLLQTLGYAEIGAYLEGRIPEAELQPLIVRRTRQFAKRQMTWFRAMLGIQKGHAYGTLSGRGRCVALWLDCEAE
DLPEQIWKQVKAWMAAPTTVETSPAAAEHRLFDP
>Mature_354_residues
MRITLENGIPAAAASDRTEWPGLIVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYREFDIGTAKPTPAEQALWPHEL
IDIVDPCHTYTVAEFQQAAQARIAEAHRQGQTPILVGGTGLYIQSVTAGLGIPAVPPQPQLRRQLETWPPEIRYAWLQQL
DPVAAQHIHPHDAVRTLRALEIVYTTGKPASSLRRAHPPHYPILILGLRCPMPRLEQRIARRTAEMMERGWIEEVKTLRE
RYGPDLPLLQTLGYAEIGAYLEGRIPEAELQPLIVRRTRQFAKRQMTWFRAMLGIQKGHAYGTLSGRGRCVALWLDCEAE
DLPEQIWKQVKAWMAAPTTVETSPAAAEHRLFDP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=306, Percent_Identity=31.3725490196078, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1790613, Length=325, Percent_Identity=36, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=33.9100346020761, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24649830, Length=265, Percent_Identity=31.3207547169811, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SYNJB (Q2JK09)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478255.1
- ProteinModelPortal:   Q2JK09
- SMR:   Q2JK09
- STRING:   Q2JK09
- GeneID:   3902613
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2044
- TIGR:   CYB_2044
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q2JK09
- ProtClustDB:   CLSK750055
- BioCyc:   SSP321332:CYB_2044-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 39621; Mature: 39621

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITLENGIPAAAASDRTEWPGLIVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYRE
CEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
FDIGTAKPTPAEQALWPHELIDIVDPCHTYTVAEFQQAAQARIAEAHRQGQTPILVGGTG
HCCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
LYIQSVTAGLGIPAVPPQPQLRRQLETWPPEIRYAWLQQLDPVAAQHIHPHDAVRTLRAL
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHH
EIVYTTGKPASSLRRAHPPHYPILILGLRCPMPRLEQRIARRTAEMMERGWIEEVKTLRE
HEEEECCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RYGPDLPLLQTLGYAEIGAYLEGRIPEAELQPLIVRRTRQFAKRQMTWFRAMLGIQKGHA
HHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YGTLSGRGRCVALWLDCEAEDLPEQIWKQVKAWMAAPTTVETSPAAAEHRLFDP
EEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MRITLENGIPAAAASDRTEWPGLIVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYRE
CEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
FDIGTAKPTPAEQALWPHELIDIVDPCHTYTVAEFQQAAQARIAEAHRQGQTPILVGGTG
HCCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
LYIQSVTAGLGIPAVPPQPQLRRQLETWPPEIRYAWLQQLDPVAAQHIHPHDAVRTLRAL
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHH
EIVYTTGKPASSLRRAHPPHYPILILGLRCPMPRLEQRIARRTAEMMERGWIEEVKTLRE
HEEEECCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RYGPDLPLLQTLGYAEIGAYLEGRIPEAELQPLIVRRTRQFAKRQMTWFRAMLGIQKGHA
HHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YGTLSGRGRCVALWLDCEAEDLPEQIWKQVKAWMAAPTTVETSPAAAEHRLFDP
EEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA