Click here to switch to the map view.

The map label for this gene is menD

Identifier: 86609492

GI number: 86609492

Start: 2144573

End: 2146399

Strand: Direct

Name: menD

Synonym: CYB_2045

Alternate gene names: 86609492

Gene position: 2144573-2146399 (Clockwise)

Preceding gene: 86609491

Following gene: 86609494

Centisome position: 70.39

GC content: 63.27

Gene sequence:

>1827_bases
GTGACCGGTGAAAACTTTGTCCTGCCGAGGCGGCTGGACTGGCGCAACCTCAACACCCTCTGGGGATCCCTATTGGCCGA
AACCTTGAGTCGGCTGGGTTGCCGTTTAGCGGTGGTGTCTCCGGGATCCCGCTCAGCCCCCCTGACCTACGCCCTGGCCC
AGCACCCCAACATCGAGGCGATCCCGGTGCTGGATGAGCGGTCGGCAGCCTTTTTTGCTCTGGGGTTGGCCCAGCAAACC
CGGCAGCCCATCCCCCTGGTGTGTACTTCCGGCAGCGCTGGCGCTCACTTTTTCCCAGCTCTCATCGAGGCGGCAGAAAG
TGGCTGGCCGCTTTTGGTTTTGACGGCGGATCGGCCCCCGGAGCTGCGCCACTGCCGCGCCGGCCAAGCGATTGACCAGG
TGAAGCTCTACGGCCATTACCCCAACTGGCAGGCGGAGTTGGCCCTGCCCAGCGCGGATCCAGAAGCCCTGGCCTACTTG
CGCCAGACCCTGGTTCACGCCTGGGAGCGCACCCTTTACCCCTTCCCCGGCCCTGTGCATTTGAATGTGCCCTTCCGGGA
TCCCTTGGCTCCCATTGCCGATGGCAGCACTGCGCCGCTGCTCCATCTACAGGAAGATCCGGAGTTCGAGAAGACCTTTT
TTCGTGGGGTTGCTCCTCCATGCACCGGCTCTTCCTTGCAGGCGGTGCGGAGCCCTTTTCAGACGGAGACGGTTTCTTTT
TGGCAGGAGTGGCGCGGCTGTGAGCGGGGGGTGATCTTGGCGGGGCCGGCTCAACCGGCGGATCCCCAGCGTTATGTGGA
GGCAGTGGCCCATTTGGCGACGGCTTTGGGGTGGCCGGTTCTGGCCGAGGGGCTCTCTCCCCTGCGCAACTACGCTTCCC
TCAACCCCTATCTCATCACCACCTACGATCTCATCCTGCAGCGGGATCGGCCCCAGCTTTGGCCGGAGCAAGTCATCCAA
CTGGGATCCCTGCCCACCAGCAAAGCGCTGCGGCAAACCCTGGCCAAGGTTGCTCCCCGCGTTTGGCGGTTGGATCCCCG
TCCCGACAATGGGGATCCCCTGCACAACCCCACCACCCACTTGCCTTTCGCCGTAGAGGAGTGGGTCGCCGGGATCCCGG
AAGCGCCGGCCAAGCGAACAACTTTTCTGCAGGGCTGGCTGACAGCGGAAGCACAAATCCGTGCTGCCCTAGACCAGGCC
ATGACTGACCTATCGGAGTGGTTTGAAGGCAAGGTTGCCTGGTGGCTGGCCCACCACTTGCCCCCAGAGACTCCGCTGTT
TATTGCCAACAGCACGCCGGTGCGGGATGTGGAGTGGTTTTGGCCCCCCAACGACCGCCGCATCCGTCCCTACTTCAATC
GCGGCACCAACGGCATCGATGGCATTCTCTCTACCGCCCTTGGGGTGGCCCACGGCTCTCACAAACGCCCAACGGTGCTG
CTGACCGGGGATCTGGCCTTTCTCCACGACAGCAGTGGCCTACGGCTCGGCCAACAGGTGCGGGGATCCCTGACCATTCT
CCTCATCAACAACCAGGGGGGTGGGATTTTCGAGCTGCTGCCCATTGCTGAAGTGGGCCGTCAAGGGCCAGATCCGCCCT
TTGAGCCCTACTTTGCTACTCCCCAGTTGGTGGATCTTGCCCTCCTCTGCCAAGCCCACGGCATTCCCCACCGGCGGGTG
GAAAGCTGGCAGGAGCTGGCCCAAGCTTTGGATCCCTTGCCTGCCCAAGGGGTGCGGGTGTTGGAGATCCTTACGGATCG
AAAAGCCGATGCTCAGCGGCGGCGGGACTGGTTTCAACAGTGGTCGGAGCTGCCATCCAAGCCTTGA

Upstream 100 bases:

>100_bases
TTCAGGAGGCGGCAGCGGATGAGTATTTTCGGCTGCACAATGCCCGCATTCACTTTTTGCCGCCGGGATCCCGATTCGAT
CTCAACACCTTGTCTCCGCT

Downstream 100 bases:

>100_bases
CCTGCTTCCAGATCTGCTCCGGCAAATCCTCAGCTTCGCAGTCCAGCCACAGGGCCACGCATCGACCCCTTCCCGACAGG
GTGCCGTAGGCATGGCCCTT

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MTGENFVLPRRLDWRNLNTLWGSLLAETLSRLGCRLAVVSPGSRSAPLTYALAQHPNIEAIPVLDERSAAFFALGLAQQT
RQPIPLVCTSGSAGAHFFPALIEAAESGWPLLVLTADRPPELRHCRAGQAIDQVKLYGHYPNWQAELALPSADPEALAYL
RQTLVHAWERTLYPFPGPVHLNVPFRDPLAPIADGSTAPLLHLQEDPEFEKTFFRGVAPPCTGSSLQAVRSPFQTETVSF
WQEWRGCERGVILAGPAQPADPQRYVEAVAHLATALGWPVLAEGLSPLRNYASLNPYLITTYDLILQRDRPQLWPEQVIQ
LGSLPTSKALRQTLAKVAPRVWRLDPRPDNGDPLHNPTTHLPFAVEEWVAGIPEAPAKRTTFLQGWLTAEAQIRAALDQA
MTDLSEWFEGKVAWWLAHHLPPETPLFIANSTPVRDVEWFWPPNDRRIRPYFNRGTNGIDGILSTALGVAHGSHKRPTVL
LTGDLAFLHDSSGLRLGQQVRGSLTILLINNQGGGIFELLPIAEVGRQGPDPPFEPYFATPQLVDLALLCQAHGIPHRRV
ESWQELAQALDPLPAQGVRVLEILTDRKADAQRRRDWFQQWSELPSKP

Sequences:

>Translated_608_residues
MTGENFVLPRRLDWRNLNTLWGSLLAETLSRLGCRLAVVSPGSRSAPLTYALAQHPNIEAIPVLDERSAAFFALGLAQQT
RQPIPLVCTSGSAGAHFFPALIEAAESGWPLLVLTADRPPELRHCRAGQAIDQVKLYGHYPNWQAELALPSADPEALAYL
RQTLVHAWERTLYPFPGPVHLNVPFRDPLAPIADGSTAPLLHLQEDPEFEKTFFRGVAPPCTGSSLQAVRSPFQTETVSF
WQEWRGCERGVILAGPAQPADPQRYVEAVAHLATALGWPVLAEGLSPLRNYASLNPYLITTYDLILQRDRPQLWPEQVIQ
LGSLPTSKALRQTLAKVAPRVWRLDPRPDNGDPLHNPTTHLPFAVEEWVAGIPEAPAKRTTFLQGWLTAEAQIRAALDQA
MTDLSEWFEGKVAWWLAHHLPPETPLFIANSTPVRDVEWFWPPNDRRIRPYFNRGTNGIDGILSTALGVAHGSHKRPTVL
LTGDLAFLHDSSGLRLGQQVRGSLTILLINNQGGGIFELLPIAEVGRQGPDPPFEPYFATPQLVDLALLCQAHGIPHRRV
ESWQELAQALDPLPAQGVRVLEILTDRKADAQRRRDWFQQWSELPSKP
>Mature_607_residues
TGENFVLPRRLDWRNLNTLWGSLLAETLSRLGCRLAVVSPGSRSAPLTYALAQHPNIEAIPVLDERSAAFFALGLAQQTR
QPIPLVCTSGSAGAHFFPALIEAAESGWPLLVLTADRPPELRHCRAGQAIDQVKLYGHYPNWQAELALPSADPEALAYLR
QTLVHAWERTLYPFPGPVHLNVPFRDPLAPIADGSTAPLLHLQEDPEFEKTFFRGVAPPCTGSSLQAVRSPFQTETVSFW
QEWRGCERGVILAGPAQPADPQRYVEAVAHLATALGWPVLAEGLSPLRNYASLNPYLITTYDLILQRDRPQLWPEQVIQL
GSLPTSKALRQTLAKVAPRVWRLDPRPDNGDPLHNPTTHLPFAVEEWVAGIPEAPAKRTTFLQGWLTAEAQIRAALDQAM
TDLSEWFEGKVAWWLAHHLPPETPLFIANSTPVRDVEWFWPPNDRRIRPYFNRGTNGIDGILSTALGVAHGSHKRPTVLL
TGDLAFLHDSSGLRLGQQVRGSLTILLINNQGGGIFELLPIAEVGRQGPDPPFEPYFATPQLVDLALLCQAHGIPHRRVE
SWQELAQALDPLPAQGVRVLEILTDRKADAQRRRDWFQQWSELPSKP

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=593, Percent_Identity=32.2091062394604, Blast_Score=220, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_SYNJB (Q2JK10)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478254.1
- ProteinModelPortal:   Q2JK10
- STRING:   Q2JK10
- GeneID:   3902612
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2045
- TIGR:   CYB_2045
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   Q2JK10
- ProtClustDB:   PRK07449
- BioCyc:   SSP321332:CYB_2045-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 67557; Mature: 67425

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGENFVLPRRLDWRNLNTLWGSLLAETLSRLGCRLAVVSPGSRSAPLTYALAQHPNIEA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCE
IPVLDERSAAFFALGLAQQTRQPIPLVCTSGSAGAHFFPALIEAAESGWPLLVLTADRPP
EEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCC
ELRHCRAGQAIDQVKLYGHYPNWQAELALPSADPEALAYLRQTLVHAWERTLYPFPGPVH
HHHHHHCCCCHHHEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
LNVPFRDPLAPIADGSTAPLLHLQEDPEFEKTFFRGVAPPCTGSSLQAVRSPFQTETVSF
EECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHH
WQEWRGCERGVILAGPAQPADPQRYVEAVAHLATALGWPVLAEGLSPLRNYASLNPYLIT
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEE
TYDLILQRDRPQLWPEQVIQLGSLPTSKALRQTLAKVAPRVWRLDPRPDNGDPLHNPTTH
EEHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCC
LPFAVEEWVAGIPEAPAKRTTFLQGWLTAEAQIRAALDQAMTDLSEWFEGKVAWWLAHHL
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCC
PPETPLFIANSTPVRDVEWFWPPNDRRIRPYFNRGTNGIDGILSTALGVAHGSHKRPTVL
CCCCCEEEECCCCCCCEEEEECCCCCEECCHHCCCCCHHHHHHHHHHHHHCCCCCCCEEE
LTGDLAFLHDSSGLRLGQQVRGSLTILLINNQGGGIFELLPIAEVGRQGPDPPFEPYFAT
EECCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCEEEECCHHHHCCCCCCCCCCCCCCC
PQLVDLALLCQAHGIPHRRVESWQELAQALDPLPAQGVRVLEILTDRKADAQRRRDWFQQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
WSELPSKP
HHHCCCCC
>Mature Secondary Structure 
TGENFVLPRRLDWRNLNTLWGSLLAETLSRLGCRLAVVSPGSRSAPLTYALAQHPNIEA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCE
IPVLDERSAAFFALGLAQQTRQPIPLVCTSGSAGAHFFPALIEAAESGWPLLVLTADRPP
EEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCC
ELRHCRAGQAIDQVKLYGHYPNWQAELALPSADPEALAYLRQTLVHAWERTLYPFPGPVH
HHHHHHCCCCHHHEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
LNVPFRDPLAPIADGSTAPLLHLQEDPEFEKTFFRGVAPPCTGSSLQAVRSPFQTETVSF
EECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHH
WQEWRGCERGVILAGPAQPADPQRYVEAVAHLATALGWPVLAEGLSPLRNYASLNPYLIT
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEE
TYDLILQRDRPQLWPEQVIQLGSLPTSKALRQTLAKVAPRVWRLDPRPDNGDPLHNPTTH
EEHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCC
LPFAVEEWVAGIPEAPAKRTTFLQGWLTAEAQIRAALDQAMTDLSEWFEGKVAWWLAHHL
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCC
PPETPLFIANSTPVRDVEWFWPPNDRRIRPYFNRGTNGIDGILSTALGVAHGSHKRPTVL
CCCCCEEEECCCCCCCEEEEECCCCCEECCHHCCCCCHHHHHHHHHHHHHCCCCCCCEEE
LTGDLAFLHDSSGLRLGQQVRGSLTILLINNQGGGIFELLPIAEVGRQGPDPPFEPYFAT
EECCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCEEEECCHHHHCCCCCCCCCCCCCCC
PQLVDLALLCQAHGIPHRRVESWQELAQALDPLPAQGVRVLEILTDRKADAQRRRDWFQQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
WSELPSKP
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA