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The map label for this gene is leuD [H]

Identifier: 86609470

GI number: 86609470

Start: 2125297

End: 2125899

Strand: Direct

Name: leuD [H]

Synonym: CYB_2021

Alternate gene names: 86609470

Gene position: 2125297-2125899 (Clockwise)

Preceding gene: 86609469

Following gene: 86609471

Centisome position: 69.76

GC content: 61.19

Gene sequence:

>603_bases
ATGTCAAGCGAAGTTTTGACCGTCACAGGCCGAGGGATCCCCCTGCTGGGCAACGACATCGACACCGATCGGATTATCCC
GGCCCGCTTTTTGAAGTGCGTCACTTTTGACGGCTTGGGCCAGCACGTGTTTGCCGACGACCGCGCCCAGGATCCCGGCC
AACACCCCTTCGATCAGCCTCAGTACCAGGGATCCCAGATCCTTGTGGTCAATGCCAATTTTGGCTGTGGCTCCAGCCGG
GAGCATGCCCCCCAAGCGCTGGCCAAATGGGGGATCCGCGCCCTCATTGGCGAAAGCTTTGCCGAGATCTTCTTCGGCAA
CTGCTTGGCCCTAGGGATCCCTTGTGTTACGGCACCCCCGGCTGTGATTCAAGCCCTGCAGAAGGCGGTGGCGGAAAATC
CCCAGCTCCAGTTGACACTGGATCTGCCCAGCAAACAGGTGTGCTGGGGGGAGAACGCCGCTGCTGTGGAGATCCCCCAG
GGAGCCTGGCAAATGCTGGTGCAGGGCACCTGGGACGCCACAGGACAACTGCTGGCCGCCCAAGCCCAGATTCAGCAAGT
AGCCGCTTCTTTACCCTACGTGCGTTGGCAGCGAGCCCGTTGA

Upstream 100 bases:

>100_bases
AAGCCTTCCCCTTTTGCCGGCTTTCCTTTGCCGTTGGGCAGGGTTTTGCCGAACGTTGCGATACCCTGAAGTCCTCTCTG
GGAGAATTCATCAAGTTCCC

Downstream 100 bases:

>100_bases
GCAATCCTCTCCCCCCTGATACCCATACATGGCTGGGCAGTGCCGACCCGGATTTTATGGATTTGGCGATCCGGGAGATA
GAAGCTGCGGATCCAGAGGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSSEVLTVTGRGIPLLGNDIDTDRIIPARFLKCVTFDGLGQHVFADDRAQDPGQHPFDQPQYQGSQILVVNANFGCGSSR
EHAPQALAKWGIRALIGESFAEIFFGNCLALGIPCVTAPPAVIQALQKAVAENPQLQLTLDLPSKQVCWGENAAAVEIPQ
GAWQMLVQGTWDATGQLLAAQAQIQQVAASLPYVRWQRAR

Sequences:

>Translated_200_residues
MSSEVLTVTGRGIPLLGNDIDTDRIIPARFLKCVTFDGLGQHVFADDRAQDPGQHPFDQPQYQGSQILVVNANFGCGSSR
EHAPQALAKWGIRALIGESFAEIFFGNCLALGIPCVTAPPAVIQALQKAVAENPQLQLTLDLPSKQVCWGENAAAVEIPQ
GAWQMLVQGTWDATGQLLAAQAQIQQVAASLPYVRWQRAR
>Mature_199_residues
SSEVLTVTGRGIPLLGNDIDTDRIIPARFLKCVTFDGLGQHVFADDRAQDPGQHPFDQPQYQGSQILVVNANFGCGSSRE
HAPQALAKWGIRALIGESFAEIFFGNCLALGIPCVTAPPAVIQALQKAVAENPQLQLTLDLPSKQVCWGENAAAVEIPQG
AWQMLVQGTWDATGQLLAAQAQIQQVAASLPYVRWQRAR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=37.3737373737374, Blast_Score=121, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321429, Length=150, Percent_Identity=35.3333333333333, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21601; Mature: 21470

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEVLTVTGRGIPLLGNDIDTDRIIPARFLKCVTFDGLGQHVFADDRAQDPGQHPFDQP
CCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCC
QYQGSQILVVNANFGCGSSREHAPQALAKWGIRALIGESFAEIFFGNCLALGIPCVTAPP
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCH
AVIQALQKAVAENPQLQLTLDLPSKQVCWGENAAAVEIPQGAWQMLVQGTWDATGQLLAA
HHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCEEEECCHHHHHHHCCCCCCHHHHHHH
QAQIQQVAASLPYVRWQRAR
HHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
SSEVLTVTGRGIPLLGNDIDTDRIIPARFLKCVTFDGLGQHVFADDRAQDPGQHPFDQP
CCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCC
QYQGSQILVVNANFGCGSSREHAPQALAKWGIRALIGESFAEIFFGNCLALGIPCVTAPP
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCH
AVIQALQKAVAENPQLQLTLDLPSKQVCWGENAAAVEIPQGAWQMLVQGTWDATGQLLAA
HHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCEEEECCHHHHHHHCCCCCCHHHHHHH
QAQIQQVAASLPYVRWQRAR
HHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]