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The map label for this gene is pdhB [H]

Identifier: 86609223

GI number: 86609223

Start: 1849211

End: 1850191

Strand: Reverse

Name: pdhB [H]

Synonym: CYB_1765

Alternate gene names: 86609223

Gene position: 1850191-1849211 (Counterclockwise)

Preceding gene: 86609227

Following gene: 86609222

Centisome position: 60.73

GC content: 56.98

Gene sequence:

>981_bases
ATGGCCGAGACATTCCTTTACAACGCCTTGCGTGCTGCCCTCGACGAAGAAATGGCCCGGGATCCCAACGTATTTGTTTT
GGGAGAAGATGTCGGCCATTACGGCGGCTCCTACAAAGTCACCAAAGACCTGTATCGCAAGTACGGGGAGATGCGCCTGC
TGGATACCCCCATTTGCGAGAACAGCTTCACCGGCCTGGCCATCGGGGCAGCCATGACCGGCTTGCGCCCGGTGGTGGAA
GGGATGAACATGGGCTTTCTGCTGCTGGCCTTCAACCAAATTGCCAACAATGCCGGTATGCTGCGCTACACTTCCGGGGG
CAATTTCAAGATCCCGATGGTCATCCGCGGGCCGGGCGGGGTGGGTCGTCAGTTGGGAGCAGAACACTCGCAGCGGCTAG
AGGCCTACTTCCAAGCGGTGCCCGGGCTTAAGATCGTGGCCTGTTCAACTCCCTATAATGCCAAGGGCTTGCTCAAGTCC
GCCATCCGCGACGACAACCCCGTTCTCTTTTTCGAGCATGTGCTCCTGTACAACTTGAAAGAAGATCTGCCAGAAGAGGA
ATACCTTTTGCCTCTGGACAAAGCCGAAATCGTCCGATCCGGCTCCGATGTTACCCTGCTCACCTACTCCCGCATGCGCT
ACCACGTGCTCAAGGCCGTGGACACTTTGGTGCAGCAGGAGATCGACCCTGAGGTGATCGATTTAATCTCGCTGAAACCT
TTAGATATGGGAACCATTGCCGCTTCGGTGCGCAAAACCCATCGGGTGATCATTGTTGAGGAAGACATGAAATCGGGTGG
CATTGGGGCGGAACTGACAGCTCGCATCATGGAAGAACTGTTCGATGAGCTGGATGCGCCGGTGATACGTTTGGCTTCGC
AGGACATTCCCACCCCCTACAACGGCACGCTGGAGGCGGCCACCATCGTGCAACCTGCAGATATTGTGGCGGCGGTGGAG
CGGCTCCTCTACGCGGATTAG

Upstream 100 bases:

>100_bases
CCCTTTGAGCAAGAATTCTGAGCTCAGCCTATCAAGTCAGCTGCAAGGGGATCCATGCCGGCAAAATGGTTTAATGAGGG
TTTAGCGATATTGAACCTCT

Downstream 100 bases:

>100_bases
AACCCCCAAAAATCCCTACAAAACAGCGCCGAAATCTATCTCCCAGCAACTTGCAAAATCCTCAGGGCAACCTTGCTAAG
ATTTCCATGGCAGGGATCCG

Product: dehydrogenase, E1 component, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MAETFLYNALRAALDEEMARDPNVFVLGEDVGHYGGSYKVTKDLYRKYGEMRLLDTPICENSFTGLAIGAAMTGLRPVVE
GMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGGVGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKS
AIRDDNPVLFFEHVLLYNLKEDLPEEEYLLPLDKAEIVRSGSDVTLLTYSRMRYHVLKAVDTLVQQEIDPEVIDLISLKP
LDMGTIAASVRKTHRVIIVEEDMKSGGIGAELTARIMEELFDELDAPVIRLASQDIPTPYNGTLEAATIVQPADIVAAVE
RLLYAD

Sequences:

>Translated_326_residues
MAETFLYNALRAALDEEMARDPNVFVLGEDVGHYGGSYKVTKDLYRKYGEMRLLDTPICENSFTGLAIGAAMTGLRPVVE
GMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGGVGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKS
AIRDDNPVLFFEHVLLYNLKEDLPEEEYLLPLDKAEIVRSGSDVTLLTYSRMRYHVLKAVDTLVQQEIDPEVIDLISLKP
LDMGTIAASVRKTHRVIIVEEDMKSGGIGAELTARIMEELFDELDAPVIRLASQDIPTPYNGTLEAATIVQPADIVAAVE
RLLYAD
>Mature_325_residues
AETFLYNALRAALDEEMARDPNVFVLGEDVGHYGGSYKVTKDLYRKYGEMRLLDTPICENSFTGLAIGAAMTGLRPVVEG
MNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGGVGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKSA
IRDDNPVLFFEHVLLYNLKEDLPEEEYLLPLDKAEIVRSGSDVTLLTYSRMRYHVLKAVDTLVQQEIDPEVIDLISLKPL
DMGTIAASVRKTHRVIIVEEDMKSGGIGAELTARIMEELFDELDAPVIRLASQDIPTPYNGTLEAATIVQPADIVAAVER
LLYAD

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=321, Percent_Identity=43.3021806853583, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI291084858, Length=321, Percent_Identity=41.1214953271028, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI4557353, Length=299, Percent_Identity=39.1304347826087, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI34101272, Length=299, Percent_Identity=39.1304347826087, Blast_Score=224, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17538422, Length=321, Percent_Identity=43.3021806853583, Blast_Score=283, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI17506935, Length=299, Percent_Identity=37.123745819398, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319698, Length=321, Percent_Identity=44.2367601246106, Blast_Score=271, Evalue=8e-74,
Organism=Drosophila melanogaster, GI21358145, Length=321, Percent_Identity=45.4828660436137, Blast_Score=294, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24650940, Length=321, Percent_Identity=45.4828660436137, Blast_Score=294, Evalue=7e-80,
Organism=Drosophila melanogaster, GI160714832, Length=299, Percent_Identity=40.4682274247492, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI160714828, Length=299, Percent_Identity=40.4682274247492, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24650943, Length=86, Percent_Identity=53.4883720930233, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650945, Length=86, Percent_Identity=53.4883720930233, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35901; Mature: 35770

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETFLYNALRAALDEEMARDPNVFVLGEDVGHYGGSYKVTKDLYRKYGEMRLLDTPICE
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHHHCCEEEECCCCCC
NSFTGLAIGAAMTGLRPVVEGMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGG
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKSAIRDDNPVLFFEHVLLYNLK
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EDLPEEEYLLPLDKAEIVRSGSDVTLLTYSRMRYHVLKAVDTLVQQEIDPEVIDLISLKP
HHCCCCCEECCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
LDMGTIAASVRKTHRVIIVEEDMKSGGIGAELTARIMEELFDELDAPVIRLASQDIPTPY
CCHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
NGTLEAATIVQPADIVAAVERLLYAD
CCCCCCEEEECHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AETFLYNALRAALDEEMARDPNVFVLGEDVGHYGGSYKVTKDLYRKYGEMRLLDTPICE
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHHHCCEEEECCCCCC
NSFTGLAIGAAMTGLRPVVEGMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGG
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKSAIRDDNPVLFFEHVLLYNLK
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EDLPEEEYLLPLDKAEIVRSGSDVTLLTYSRMRYHVLKAVDTLVQQEIDPEVIDLISLKP
HHCCCCCEECCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
LDMGTIAASVRKTHRVIIVEEDMKSGGIGAELTARIMEELFDELDAPVIRLASQDIPTPY
CCHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
NGTLEAATIVQPADIVAAVERLLYAD
CCCCCCEEEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]