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The map label for this gene is rsmE [H]

Identifier: 86608181

GI number: 86608181

Start: 713301

End: 714170

Strand: Reverse

Name: rsmE [H]

Synonym: CYB_0695

Alternate gene names: 86608181

Gene position: 714170-713301 (Counterclockwise)

Preceding gene: 86608182

Following gene: 86608180

Centisome position: 23.44

GC content: 63.22

Gene sequence:

>870_bases
ATGGGTGGTGGCAGCCCTCTGGCGACGGGGGGAATGGTGTTGTCTGAACGCACGCATGACCTGAGCGCTTTGCCCCGGTT
GGCGATTGACCCGGCGCAGCGGGATCCGAGTGGTGTTCTCCGTTTAACCGGCCCGCAACAGCACTACTTGCAGCGGGTGC
GCCGCCTCAGGCCAGGGGATCTCTTTTTGGTTTTGGACGGCTCAGGCAAGCTGTGGGCGGCCCGTTGGCAAGCTCAGGGA
TCCCAAATTGTGGGCGAGGTGGAGACGCTGCCGCGGGAATTGCCCGTTTTTTTGCATCTGGGCTTGGCGGTGGTCAAAGG
GCAGGGGTTTGACGAGGTGCTCCGCCAGGTTACCGAGCTGGGGGTGGCGCGAATTACCCCCCTGCTGACAGAGCGCACCG
TGTTGGAACCGGGATCTGGGCGGCAGGAACGCTGGCAAAAAATTGTGCGGGAGGCCGCAGAACAATGTGAGCGGTTGCGC
TGGCCTCAGGTCGATCCCCCCACCCCTTGGACAAGTTGGCTGGCGGAGCTGCAAGCCGTTCGCGCTACTCGGACGGAGTC
CTTCTGGTGGGGTATAGCCGTGGCGCGCGAGAACGCCCCCCTGCTGGTGCAGGCCCTGCCAAAGCTGCAGATCCCGGCCT
TGGCCATAGCCATTGGCCCAGAAGGGGGCTGGACGGTTGCCGAACGTCGGCAGGCTGAGGAATATGGGGGAGTGTCCGTC
TCCCTTGGCCCTACCATCTTGCGAGCCACCACCGCCGCTGTTGTTGCCACCAGCCAAATTGCCGCCACCTACTCCTGGTT
GGACTCGAGCTTGGATCGGGGTCATTTGGCTCCGCTAGCGACGGGGAGTGTTGTCGCCGATAATCAATAA

Upstream 100 bases:

>100_bases
CGGAGTTTCGGCTGGTGGTGAAGGAGGGGGGATGGCTGATCCTCAGCGGCATCCTCATCGAGCAGGCCAAGTTGGTGGCG
GAGATGCTGGAGGCCAACGA

Downstream 100 bases:

>100_bases
CTAGGCCGTTTGCGTCAGCGGGACGGAGTCCTTTGACGAGAACAGCCATTGGCATAGCAGGCGGCCAGGCCCACCCCAGG
GAGGGAGAACGAGAATGGAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MGGGSPLATGGMVLSERTHDLSALPRLAIDPAQRDPSGVLRLTGPQQHYLQRVRRLRPGDLFLVLDGSGKLWAARWQAQG
SQIVGEVETLPRELPVFLHLGLAVVKGQGFDEVLRQVTELGVARITPLLTERTVLEPGSGRQERWQKIVREAAEQCERLR
WPQVDPPTPWTSWLAELQAVRATRTESFWWGIAVARENAPLLVQALPKLQIPALAIAIGPEGGWTVAERRQAEEYGGVSV
SLGPTILRATTAAVVATSQIAATYSWLDSSLDRGHLAPLATGSVVADNQ

Sequences:

>Translated_289_residues
MGGGSPLATGGMVLSERTHDLSALPRLAIDPAQRDPSGVLRLTGPQQHYLQRVRRLRPGDLFLVLDGSGKLWAARWQAQG
SQIVGEVETLPRELPVFLHLGLAVVKGQGFDEVLRQVTELGVARITPLLTERTVLEPGSGRQERWQKIVREAAEQCERLR
WPQVDPPTPWTSWLAELQAVRATRTESFWWGIAVARENAPLLVQALPKLQIPALAIAIGPEGGWTVAERRQAEEYGGVSV
SLGPTILRATTAAVVATSQIAATYSWLDSSLDRGHLAPLATGSVVADNQ
>Mature_288_residues
GGGSPLATGGMVLSERTHDLSALPRLAIDPAQRDPSGVLRLTGPQQHYLQRVRRLRPGDLFLVLDGSGKLWAARWQAQGS
QIVGEVETLPRELPVFLHLGLAVVKGQGFDEVLRQVTELGVARITPLLTERTVLEPGSGRQERWQKIVREAAEQCERLRW
PQVDPPTPWTSWLAELQAVRATRTESFWWGIAVARENAPLLVQALPKLQIPALAIAIGPEGGWTVAERRQAEEYGGVSVS
LGPTILRATTAAVVATSQIAATYSWLDSSLDRGHLAPLATGSVVADNQ

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=229, Percent_Identity=30.1310043668122, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 31425; Mature: 31294

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGGSPLATGGMVLSERTHDLSALPRLAIDPAQRDPSGVLRLTGPQQHYLQRVRRLRPGD
CCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCC
LFLVLDGSGKLWAARWQAQGSQIVGEVETLPRELPVFLHLGLAVVKGQGFDEVLRQVTEL
EEEEECCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GVARITPLLTERTVLEPGSGRQERWQKIVREAAEQCERLRWPQVDPPTPWTSWLAELQAV
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RATRTESFWWGIAVARENAPLLVQALPKLQIPALAIAIGPEGGWTVAERRQAEEYGGVSV
HHHCCCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEE
SLGPTILRATTAAVVATSQIAATYSWLDSSLDRGHLAPLATGSVVADNQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
GGGSPLATGGMVLSERTHDLSALPRLAIDPAQRDPSGVLRLTGPQQHYLQRVRRLRPGD
CCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCC
LFLVLDGSGKLWAARWQAQGSQIVGEVETLPRELPVFLHLGLAVVKGQGFDEVLRQVTEL
EEEEECCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GVARITPLLTERTVLEPGSGRQERWQKIVREAAEQCERLRWPQVDPPTPWTSWLAELQAV
HHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RATRTESFWWGIAVARENAPLLVQALPKLQIPALAIAIGPEGGWTVAERRQAEEYGGVSV
HHHCCCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEE
SLGPTILRATTAAVVATSQIAATYSWLDSSLDRGHLAPLATGSVVADNQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]