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The map label for this gene is nifE [H]

Identifier: 86607908

GI number: 86607908

Start: 421901

End: 423295

Strand: Reverse

Name: nifE [H]

Synonym: CYB_0411

Alternate gene names: 86607908

Gene position: 423295-421901 (Counterclockwise)

Preceding gene: 86607909

Following gene: 86607907

Centisome position: 13.89

GC content: 61.65

Gene sequence:

>1395_bases
ATGCCTTTGATCAGCCGGACTAAAGTGGCGGAGCTGCTCAACGAGACCGCCTGCGAGCACAACCACAAGAAAGACGGCAA
AGGCAAAAACAAAGTCTGTGCCGAGCAAGCCAAGCCAGGAGGAGCGCAGGGAGGCTGTGCCTTTGACGGGGCTTCGATTG
CCCTGGTGCCGATCGCCGATGCCGCCCATCTAGTACATGGGCCAATCGCCTGCAGCGGCAACTCTTGGGGGACGCGGGGC
AGCCTCTCTTCTGGCCCCCAGCTTTATCGGGTGGGCTTCACCACCGATCTGAGCGAGAACGAGATCATCTTCGGTGGGGA
GGAGAAGCTGTTCCAGGCCATCCTTGACTTGCAGGCCAAATACCAGCCTGCCGCCATTTTTGTCTATCTCACTTGTGTAA
CTGCCCTCATTGGCGACGATGTGGAGGCCGTGTGTCAGCGGGCCAGCCGCCAGCTTGGGATCCCGGTCATTCCGGTGAAT
GCGCCCGGCTTTGTGGGCAGCAAAAACCTAGGCAACCGTCTGGCCGGAGAAGCCCTGCTGGACTATGTTATCGGCACCGC
CTCCCCGGAGTACACCACTCCCCGCGACATTAACCTCATCGGCGAGTACAACATTGCCGGCGAGCTGTGGGATGTCTTGC
CTCTGTTTGACGAGCTGGGGATCCGCATCTTGGCCAAGATCACCGGCGATGGCCGCTACCGCGAGATCTGTACCGCCCAC
CGGGCCAAGCTGAACGTCCTCATCTGCGCCAAAGCCCTGCTCAATGTGGGCCGCAAGATGCAGGAGCGCTACGGGATCCC
CTACATTGAGGCTTCCTTTTACGGGCTGCAGGAGATGAACCACTGCCTACGGGCCATTGCCCAAGGGCTGAAGGATCCCG
AACTGCAGGAGCGTACCGAGGCCCTCATCGAGCGGGAAACTCGCGCCACCGAGGCGGCTTTGGCCCCCTACCGGCAGCGG
CTGGCGGGCAAACGAGTTGTCCTTTATACCGGCGGCGTGAAAAGTTGGTCGATCATCTCTGCTGCCCGAGATCTGGGGAT
GCAGGTAACGGCCACCAGCACCCGCAAAAGCACCGAAGAAGACAAAGCCCGCATCCGCGAGCTGCTCGGCCAGGATGGCA
TTCTCTTGGACAAGGGCAGCCCTCAAGAGCTGCTCAAGGTGGTGGAGCGCACGAAAGCGGACTTGCTCATCGCCGGCGGA
CGCAACCAGTACACCGCCCTCAAGGCCCGCATCCCCTTTCTGGACATCAACCAGGAGCGGCACCATGCCTATGCCGGCTA
TCGCGGCCTGGTGAACTTGGCGCGGGAGATCGATGCCGCCCTCCACAGCCCCATCTGGGAGCAGTTGCGACGGCCTGCCC
CCTGGGAGACTCCAGCCGAAGAGGAGGTGGGATGA

Upstream 100 bases:

>100_bases
CCCTCTACGAGGAGATCATCGCCCTGCGGCTACGGGCGGCGGCTTATCTGGCCGAAGCTGCGGCTCAACCCCAGCCCCCC
TCTTCTCAGGAGATCCCATC

Downstream 100 bases:

>100_bases
TGGCCCAGCTCATCCAAGAACGCAAGCCTCTCAGCGTCAACCCCCTCAAACTCAGCGCTCCGCTGGGAGCGGCCCTGGCC
TTTCTGGGGATCAAGGGCTG

Product: nitrogenase MoFe cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPLISRTKVAELLNETACEHNHKKDGKGKNKVCAEQAKPGGAQGGCAFDGASIALVPIADAAHLVHGPIACSGNSWGTRG
SLSSGPQLYRVGFTTDLSENEIIFGGEEKLFQAILDLQAKYQPAAIFVYLTCVTALIGDDVEAVCQRASRQLGIPVIPVN
APGFVGSKNLGNRLAGEALLDYVIGTASPEYTTPRDINLIGEYNIAGELWDVLPLFDELGIRILAKITGDGRYREICTAH
RAKLNVLICAKALLNVGRKMQERYGIPYIEASFYGLQEMNHCLRAIAQGLKDPELQERTEALIERETRATEAALAPYRQR
LAGKRVVLYTGGVKSWSIISAARDLGMQVTATSTRKSTEEDKARIRELLGQDGILLDKGSPQELLKVVERTKADLLIAGG
RNQYTALKARIPFLDINQERHHAYAGYRGLVNLAREIDAALHSPIWEQLRRPAPWETPAEEEVG

Sequences:

>Translated_464_residues
MPLISRTKVAELLNETACEHNHKKDGKGKNKVCAEQAKPGGAQGGCAFDGASIALVPIADAAHLVHGPIACSGNSWGTRG
SLSSGPQLYRVGFTTDLSENEIIFGGEEKLFQAILDLQAKYQPAAIFVYLTCVTALIGDDVEAVCQRASRQLGIPVIPVN
APGFVGSKNLGNRLAGEALLDYVIGTASPEYTTPRDINLIGEYNIAGELWDVLPLFDELGIRILAKITGDGRYREICTAH
RAKLNVLICAKALLNVGRKMQERYGIPYIEASFYGLQEMNHCLRAIAQGLKDPELQERTEALIERETRATEAALAPYRQR
LAGKRVVLYTGGVKSWSIISAARDLGMQVTATSTRKSTEEDKARIRELLGQDGILLDKGSPQELLKVVERTKADLLIAGG
RNQYTALKARIPFLDINQERHHAYAGYRGLVNLAREIDAALHSPIWEQLRRPAPWETPAEEEVG
>Mature_463_residues
PLISRTKVAELLNETACEHNHKKDGKGKNKVCAEQAKPGGAQGGCAFDGASIALVPIADAAHLVHGPIACSGNSWGTRGS
LSSGPQLYRVGFTTDLSENEIIFGGEEKLFQAILDLQAKYQPAAIFVYLTCVTALIGDDVEAVCQRASRQLGIPVIPVNA
PGFVGSKNLGNRLAGEALLDYVIGTASPEYTTPRDINLIGEYNIAGELWDVLPLFDELGIRILAKITGDGRYREICTAHR
AKLNVLICAKALLNVGRKMQERYGIPYIEASFYGLQEMNHCLRAIAQGLKDPELQERTEALIERETRATEAALAPYRQRL
AGKRVVLYTGGVKSWSIISAARDLGMQVTATSTRKSTEEDKARIRELLGQDGILLDKGSPQELLKVVERTKADLLIAGGR
NQYTALKARIPFLDINQERHHAYAGYRGLVNLAREIDAALHSPIWEQLRRPAPWETPAEEEVG

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 50690; Mature: 50558

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLISRTKVAELLNETACEHNHKKDGKGKNKVCAEQAKPGGAQGGCAFDGASIALVPIAD
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCEECCCCEEEEEECC
AAHLVHGPIACSGNSWGTRGSLSSGPQLYRVGFTTDLSENEIIFGGEEKLFQAILDLQAK
HHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHCC
YQPAAIFVYLTCVTALIGDDVEAVCQRASRQLGIPVIPVNAPGFVGSKNLGNRLAGEALL
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHH
DYVIGTASPEYTTPRDINLIGEYNIAGELWDVLPLFDELGIRILAKITGDGRYREICTAH
HHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
RAKLNVLICAKALLNVGRKMQERYGIPYIEASFYGLQEMNHCLRAIAQGLKDPELQERTE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALIERETRATEAALAPYRQRLAGKRVVLYTGGVKSWSIISAARDLGMQVTATSTRKSTEE
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEECCCCCCCHH
DKARIRELLGQDGILLDKGSPQELLKVVERTKADLLIAGGRNQYTALKARIPFLDINQER
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCEEECCCHH
HHAYAGYRGLVNLAREIDAALHSPIWEQLRRPAPWETPAEEEVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLISRTKVAELLNETACEHNHKKDGKGKNKVCAEQAKPGGAQGGCAFDGASIALVPIAD
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCEECCCCEEEEEECC
AAHLVHGPIACSGNSWGTRGSLSSGPQLYRVGFTTDLSENEIIFGGEEKLFQAILDLQAK
HHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHCC
YQPAAIFVYLTCVTALIGDDVEAVCQRASRQLGIPVIPVNAPGFVGSKNLGNRLAGEALL
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHH
DYVIGTASPEYTTPRDINLIGEYNIAGELWDVLPLFDELGIRILAKITGDGRYREICTAH
HHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
RAKLNVLICAKALLNVGRKMQERYGIPYIEASFYGLQEMNHCLRAIAQGLKDPELQERTE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALIERETRATEAALAPYRQRLAGKRVVLYTGGVKSWSIISAARDLGMQVTATSTRKSTEE
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEECCCCCCCHH
DKARIRELLGQDGILLDKGSPQELLKVVERTKADLLIAGGRNQYTALKARIPFLDINQER
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCEEECCCHH
HHAYAGYRGLVNLAREIDAALHSPIWEQLRRPAPWETPAEEEVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10217509 [H]