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The map label for this gene is pyrF

Identifier: 86607840

GI number: 86607840

Start: 362120

End: 362827

Strand: Reverse

Name: pyrF

Synonym: CYB_0343

Alternate gene names: 86607840

Gene position: 362827-362120 (Counterclockwise)

Preceding gene: 86607841

Following gene: 86607839

Centisome position: 11.91

GC content: 61.72

Gene sequence:

>708_bases
ATGAAGGGTGACCGCCTGATTGTTGCGCTGGATACTCCTTCTCAAGAGCAGGCTTTGGAGTGGGTGGACTCCTTGCCGCA
GGTGCTGTGGTGGAAGGTGGGTCTGGAGTTGTTCACTGCTGCGGGTTCTTCCATCCTGCAGGCTCTCAAGGAAAGGGGCA
AGCGCATTTTCCTGGATTTGAAGCTGCACGACATTCCCCACACGGTGGGGAGGGCTACGGAGGTGGCCGTCGGCTATGGA
GTGGATCTGCTCACCGTCCATGCCGCCGGTGGCAGTGCTATGTTGCGCTCAGCCGTGGCAGCCGCCCAGGGCAGTTCTTG
TCGGCTGTTGGCGGTTACCCTCTTAACCAGCATCAGCCCCCCGCAGGTGCGACAGGAACTGGGCATCGAGATGGATCCCG
CCGACTATGTGTTGCATTTGGCCCGGCTGGCTCAAGCTCAAGGCTTTACTGGACTGGTTTGCTCTCCTCAGGAGGTCTCG
CGGTTGCGCCAAGCTTTGGGGCCGGAGGTTTTGCTGGTTACTCCCGGGATCCGTCTGGGCCAAGCCAAGGCGGGGGATGG
AGACGAGGATCAACAGCGCATCTGCACGCCCGACGAAGCTCTGCGGGCAGGAGCCGATTATCTGGTGGTGGGCCGCCCCA
TCACCGCTTCTCCCGATCCCGTGGCCGCCTTGCGGCGCTTCCAAGCCTTGTGGGATGCTGCTAGCTGA

Upstream 100 bases:

>100_bases
CCTGCCGCTACAATGCCCATTCTCCAGAGTTGCGCTGCGCCGTCAACCCCTGCGGCCCCTGTGAGGGCTGTCCCCACTAC
GAGCCTCGGAATGAGCCCCG

Downstream 100 bases:

>100_bases
GAGGGTGGGGAGATGGTTTGGCCTGATGTTGTCCTGGTGGTTCTGTGATGCAACGATACCATAGGCTTGTCCGGTGGATC
CCTGAAAAAAGGCCGGCTGG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKGDRLIVALDTPSQEQALEWVDSLPQVLWWKVGLELFTAAGSSILQALKERGKRIFLDLKLHDIPHTVGRATEVAVGYG
VDLLTVHAAGGSAMLRSAVAAAQGSSCRLLAVTLLTSISPPQVRQELGIEMDPADYVLHLARLAQAQGFTGLVCSPQEVS
RLRQALGPEVLLVTPGIRLGQAKAGDGDEDQQRICTPDEALRAGADYLVVGRPITASPDPVAALRRFQALWDAAS

Sequences:

>Translated_235_residues
MKGDRLIVALDTPSQEQALEWVDSLPQVLWWKVGLELFTAAGSSILQALKERGKRIFLDLKLHDIPHTVGRATEVAVGYG
VDLLTVHAAGGSAMLRSAVAAAQGSSCRLLAVTLLTSISPPQVRQELGIEMDPADYVLHLARLAQAQGFTGLVCSPQEVS
RLRQALGPEVLLVTPGIRLGQAKAGDGDEDQQRICTPDEALRAGADYLVVGRPITASPDPVAALRRFQALWDAAS
>Mature_235_residues
MKGDRLIVALDTPSQEQALEWVDSLPQVLWWKVGLELFTAAGSSILQALKERGKRIFLDLKLHDIPHTVGRATEVAVGYG
VDLLTVHAAGGSAMLRSAVAAAQGSSCRLLAVTLLTSISPPQVRQELGIEMDPADYVLHLARLAQAQGFTGLVCSPQEVS
RLRQALGPEVLLVTPGIRLGQAKAGDGDEDQQRICTPDEALRAGADYLVVGRPITASPDPVAALRRFQALWDAAS

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=44.2982456140351, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_SYNJB (Q2JPF1)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_476602.1
- ProteinModelPortal:   Q2JPF1
- SMR:   Q2JPF1
- STRING:   Q2JPF1
- GeneID:   3901284
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0343
- TIGR:   CYB_0343
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q2JPF1
- ProtClustDB:   CLSK750486
- BioCyc:   SSP321332:CYB_0343-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25103; Mature: 25103

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 11-11 BINDING 32-32 BINDING 116-116 BINDING 178-178 BINDING 191-191 BINDING 211-211 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGDRLIVALDTPSQEQALEWVDSLPQVLWWKVGLELFTAAGSSILQALKERGKRIFLDL
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEE
KLHDIPHTVGRATEVAVGYGVDLLTVHAAGGSAMLRSAVAAAQGSSCRLLAVTLLTSISP
EECCCCHHHCCHHHHHECCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
PQVRQELGIEMDPADYVLHLARLAQAQGFTGLVCSPQEVSRLRQALGPEVLLVTPGIRLG
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCEEEECCCCEEC
QAKAGDGDEDQQRICTPDEALRAGADYLVVGRPITASPDPVAALRRFQALWDAAS
CCCCCCCCCCHHHCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGDRLIVALDTPSQEQALEWVDSLPQVLWWKVGLELFTAAGSSILQALKERGKRIFLDL
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEE
KLHDIPHTVGRATEVAVGYGVDLLTVHAAGGSAMLRSAVAAAQGSSCRLLAVTLLTSISP
EECCCCHHHCCHHHHHECCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
PQVRQELGIEMDPADYVLHLARLAQAQGFTGLVCSPQEVSRLRQALGPEVLLVTPGIRLG
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCEEEECCCCEEC
QAKAGDGDEDQQRICTPDEALRAGADYLVVGRPITASPDPVAALRRFQALWDAAS
CCCCCCCCCCHHHCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA