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The map label for this gene is cysW [H]

Identifier: 86607735

GI number: 86607735

Start: 245242

End: 246069

Strand: Reverse

Name: cysW [H]

Synonym: CYB_0236

Alternate gene names: 86607735

Gene position: 246069-245242 (Counterclockwise)

Preceding gene: 86607736

Following gene: 86607732

Centisome position: 8.08

GC content: 60.27

Gene sequence:

>828_bases
ATGAAACCTGACGAGGTAGCAGGTGTCAAACCGAAGCCCTCAACATGGCTGTCGGGCCTGTTGATCGGGATCGTCGGGCT
TTACATCGCGCTGCTGCTCGGGATCCCGGTGGTGAACATCTTCTATCAGGCGTTTCGAGAAGGGATCCCAGCCTTTCTCC
ATCGCCTGACAGAGCGGGAGTTGCTCAGTGCCCTGCGATTGACGGTGCTAACCGCCCTGGTCGTTGTGCCCCTCAATACC
TTTTTCGGCCTCTGTGCTGCCTTGGCCATTGCTCATCGGCGTTTTCCGGGGCGGGCGCTGCTGCTGAGCCTGATCGATCT
GCCCTTTTCCATTTCCCCGGTGGTGGCTGGCCTAATGCTGGTGCTCATCTTCGGGCGTCAGGGTTGGCTGGGATCCTGGA
GGGTTATCTTTGCATTTCCGGGCATTGTGTTGGCAACGGCTTTCATCACCCTTCCTTTTGTGGCGCGGGAAGTGTTGCCG
GTGTTGGAGGAGGCAGGCACCGACCAGGAAGAAGCCGCCAAAACGCTGGGGGCGACCCCCTGGCAGATCTTCTGGAGAAT
CACCCTGCCCAACATCCGCTGGGGCCTTCTGTACGGCGTCATCTTGACCAATGCCCGCGCCATGGGGGAGTTCGGCGCTG
TGGCTGTGGTTTCCGGCAACATCATTGGCCGCACCCAGACCTTGCCCCTGTTCGTGGAAGATGCCTACCGCAGTTATGCC
ACCGGCTCAGCCTATGCGGCGGCGGTGGTGCTCTCCTTGCTGGCGGTGGTAACCCTAGTGGCTAGGGAGTTCCTTGAGCG
GGGATCCCATTCCCGCCGAGTCTTTTGA

Upstream 100 bases:

>100_bases
CACGACTATGCTGGGGCAACCGTGATCGGAACGGTGATGTTGGGGATCTCTTTCCTGTCGCTGGTGGTAATTAACCTTCT
GCAAGCCTGGGGAAGTCGCC

Downstream 100 bases:

>100_bases
CGGTTGGGTTGGGGCTAGGCAGGCTCGGTGAGGATCTCTGGCAGACAAACCCTAGTGCCCCGCAGGCCGCAGTAGCCGCG
CGGATTCTTGGCCAGATATT

Product: sulfate ABC transporter, permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKPDEVAGVKPKPSTWLSGLLIGIVGLYIALLLGIPVVNIFYQAFREGIPAFLHRLTERELLSALRLTVLTALVVVPLNT
FFGLCAALAIAHRRFPGRALLLSLIDLPFSISPVVAGLMLVLIFGRQGWLGSWRVIFAFPGIVLATAFITLPFVAREVLP
VLEEAGTDQEEAAKTLGATPWQIFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIIGRTQTLPLFVEDAYRSYA
TGSAYAAAVVLSLLAVVTLVAREFLERGSHSRRVF

Sequences:

>Translated_275_residues
MKPDEVAGVKPKPSTWLSGLLIGIVGLYIALLLGIPVVNIFYQAFREGIPAFLHRLTERELLSALRLTVLTALVVVPLNT
FFGLCAALAIAHRRFPGRALLLSLIDLPFSISPVVAGLMLVLIFGRQGWLGSWRVIFAFPGIVLATAFITLPFVAREVLP
VLEEAGTDQEEAAKTLGATPWQIFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIIGRTQTLPLFVEDAYRSYA
TGSAYAAAVVLSLLAVVTLVAREFLERGSHSRRVF
>Mature_275_residues
MKPDEVAGVKPKPSTWLSGLLIGIVGLYIALLLGIPVVNIFYQAFREGIPAFLHRLTERELLSALRLTVLTALVVVPLNT
FFGLCAALAIAHRRFPGRALLLSLIDLPFSISPVVAGLMLVLIFGRQGWLGSWRVIFAFPGIVLATAFITLPFVAREVLP
VLEEAGTDQEEAAKTLGATPWQIFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIIGRTQTLPLFVEDAYRSYA
TGSAYAAAVVLSLLAVVTLVAREFLERGSHSRRVF

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=261, Percent_Identity=48.2758620689655, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1788764, Length=202, Percent_Identity=42.0792079207921, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786980, Length=222, Percent_Identity=35.1351351351351, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29940; Mature: 29940

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDEVAGVKPKPSTWLSGLLIGIVGLYIALLLGIPVVNIFYQAFREGIPAFLHRLTERE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSALRLTVLTALVVVPLNTFFGLCAALAIAHRRFPGRALLLSLIDLPFSISPVVAGLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
VLIFGRQGWLGSWRVIFAFPGIVLATAFITLPFVAREVLPVLEEAGTDQEEAAKTLGATP
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
WQIFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIIGRTQTLPLFVEDAYRSYA
CEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHHHHHHC
TGSAYAAAVVLSLLAVVTLVAREFLERGSHSRRVF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKPDEVAGVKPKPSTWLSGLLIGIVGLYIALLLGIPVVNIFYQAFREGIPAFLHRLTERE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSALRLTVLTALVVVPLNTFFGLCAALAIAHRRFPGRALLLSLIDLPFSISPVVAGLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
VLIFGRQGWLGSWRVIFAFPGIVLATAFITLPFVAREVLPVLEEAGTDQEEAAKTLGATP
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
WQIFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIIGRTQTLPLFVEDAYRSYA
CEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHHHHHHC
TGSAYAAAVVLSLLAVVTLVAREFLERGSHSRRVF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]