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The map label for this gene is cysT [H]

Identifier: 86607736

GI number: 86607736

Start: 246059

End: 246871

Strand: Reverse

Name: cysT [H]

Synonym: CYB_0237

Alternate gene names: 86607736

Gene position: 246871-246059 (Counterclockwise)

Preceding gene: 86607737

Following gene: 86607735

Centisome position: 8.1

GC content: 60.02

Gene sequence:

>813_bases
TTGCTGCAAGCTTTGTCTCAACTGTCTTGGCCGTGGCGGCTGACCCTGGGCTATCTCTTCTTGATGTTGATTCTGCCGCT
GGCTGCCTTGATTGCCAAGTCCAGCAGCGTGGGCTGGGCGGAGTTTTGGCGGATCGCGACGGATCCCGTGGCCCTCTCCG
CCTATGAAGTTACTTTTGTAACCGCGTTGCTGGCGGCTCTGATCAACGGCGTGATGGGCACCCTGACTGCCTGGGTGCTG
GTGCGCTACGCCTTCCCCGGCAAAAAGCTTCTCGAGGGGTTGATCGATCTGCCGTTTGCTTTGCCCACCGCCGTGGCCGG
CCTCACCCTGGCAACGGTTTACAGCAGCAACGGCTGGATTGGATCCCTTTTGGCCCCCTTGGGGATCCGCATCTCCTTTA
CCCGCTTGGGGGTGGGGGTGGCGATGCTGTTTATTTCTTTTCCCTTTGTGGTGCGGACGGTGCAGCCGGTGTTGCAGGAG
CTGGAGAAAGATGTGGAAGAGGCAGCCTGGAGTTTGGGGGCTTCCCGCTTTCAGACCTTCCGGCGCGTGGTGCTGCCCCA
GATTCTGCCGGCAGTGCTCACCGGGGTGGCCCTGGGGTTTTCCCGCGCCGTGGGGGAATACGGCTCTGTGGTGATCATCG
CTTCCAGCATTCCCTTTCGGGACTTGATCGCGGCGGTGCTGGTGATTCAGCGGCTGGAACAACACGACTATGCTGGGGCA
ACCGTGATCGGAACGGTGATGTTGGGGATCTCTTTCCTGTCGCTGGTGGTAATTAACCTTCTGCAAGCCTGGGGAAGTCG
CCATGAAACCTGA

Upstream 100 bases:

>100_bases
GCAGGTGGGGCAGCAGGTCTATGTGCGCCCCAAGGTGGTGAAAGCCTTTCCCGTCAAAAAAACCGAGATGACCGCAATGC
CGGTGGGAGCTTGAGAAACG

Downstream 100 bases:

>100_bases
CGAGGTAGCAGGTGTCAAACCGAAGCCCTCAACATGGCTGTCGGGCCTGTTGATCGGGATCGTCGGGCTTTACATCGCGC
TGCTGCTCGGGATCCCGGTG

Product: sulfate ABC transporter, permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLQALSQLSWPWRLTLGYLFLMLILPLAALIAKSSSVGWAEFWRIATDPVALSAYEVTFVTALLAALINGVMGTLTAWVL
VRYAFPGKKLLEGLIDLPFALPTAVAGLTLATVYSSNGWIGSLLAPLGIRISFTRLGVGVAMLFISFPFVVRTVQPVLQE
LEKDVEEAAWSLGASRFQTFRRVVLPQILPAVLTGVALGFSRAVGEYGSVVIIASSIPFRDLIAAVLVIQRLEQHDYAGA
TVIGTVMLGISFLSLVVINLLQAWGSRHET

Sequences:

>Translated_270_residues
MLQALSQLSWPWRLTLGYLFLMLILPLAALIAKSSSVGWAEFWRIATDPVALSAYEVTFVTALLAALINGVMGTLTAWVL
VRYAFPGKKLLEGLIDLPFALPTAVAGLTLATVYSSNGWIGSLLAPLGIRISFTRLGVGVAMLFISFPFVVRTVQPVLQE
LEKDVEEAAWSLGASRFQTFRRVVLPQILPAVLTGVALGFSRAVGEYGSVVIIASSIPFRDLIAAVLVIQRLEQHDYAGA
TVIGTVMLGISFLSLVVINLLQAWGSRHET
>Mature_270_residues
MLQALSQLSWPWRLTLGYLFLMLILPLAALIAKSSSVGWAEFWRIATDPVALSAYEVTFVTALLAALINGVMGTLTAWVL
VRYAFPGKKLLEGLIDLPFALPTAVAGLTLATVYSSNGWIGSLLAPLGIRISFTRLGVGVAMLFISFPFVVRTVQPVLQE
LEKDVEEAAWSLGASRFQTFRRVVLPQILPAVLTGVALGFSRAVGEYGSVVIIASSIPFRDLIAAVLVIQRLEQHDYAGA
TVIGTVMLGISFLSLVVINLLQAWGSRHET

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=234, Percent_Identity=49.5726495726496, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI87082099, Length=242, Percent_Identity=36.3636363636364, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1786980, Length=175, Percent_Identity=36.5714285714286, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787369, Length=214, Percent_Identity=28.5046728971963, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787368, Length=155, Percent_Identity=30.9677419354839, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29200; Mature: 29200

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231; 8425067 [H]