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The map label for this gene is folC

Identifier: 86606025

GI number: 86606025

Start: 1363311

End: 1364600

Strand: Reverse

Name: folC

Synonym: CYA_1353

Alternate gene names: 86606025

Gene position: 1364600-1363311 (Counterclockwise)

Preceding gene: 86606026

Following gene: 86606024

Centisome position: 46.53

GC content: 65.12

Gene sequence:

>1290_bases
ATGGGTGGGATCTCAACCTGGGATTGGCCTGGGTTTCTGGAGTCGCTGGGCCACTTTGGCGTCGATCTCAGCCTGGATCG
CATGCAGGTTCTGCTGCAGCGCCTGGGCCAGCCGCAGGCGGGGATCCCAGCCATCCACGTGGCCGGCACCAACGGCAAGG
GATCCGTCTGCGCCTGGGTCAGCCACGCGCTGTGGGCGGCGGGCTATCGCGTCGGTCGCTACACCTCTCCCCACCTGGTC
AATTGGCGGGAGCGGATCTGGCTGAGCGGCGAGTACATTTCGCCAGGCGACTGGAGCCGGGTGTTGGCCCAAGTGCGAGA
TTGCCTGCAGACCTACGCTCCAGGCGAGGCTTCCCCCACCCAGTTCGAGGCCATTACCGCCGCTGCCTGGCTGTACTTTC
GGCAGCAGAAGGTGGACATTGCCGTGATGGAAGTGGGGCTGGGGGGGCGGCTGGATGCCACCAACGCCGGCATCGAGCCG
GTAGTGACCGCCATTACCTCCATCGGCTGGGATCATTGGCAGCGTCTGGGCAACAGCCTGGCGCAAATTGCCTTTGAAAA
AGCCGGCATTCTCAAGCCGGGGATCCCGACTGTGTGCGCGCCCCAACCCCCTGAGGTGATGGCCGTGATCCGGGCTAGAG
CCGAGCAGACGGGCAGCCCCCTGCGGGTGGTGGAGCCCCTGCACTGGGCCGGTCCTCAGACTGTCTGCTGGCGGGATCAG
GTGCTTACCCTGCCTCTTGCCGGTGAGGTGCAACTGATCAATGGGGCGGTAGCTCTGGCCGTTGTGGAGACCCTGCAGGA
ACAGGGTTGGCGGATCCCAGATGTCGCTGTCGAGAAAGGATTTGCCCTCACCCGTTGGCCGGGTCGCCTACAGTGGGTGC
AAATTGGCTCCCGCCAGCTGCTGCTGGATGGGGCCCACAACTTGCCCGCCGCCCAGGCGCTGCGCCGCTACCTAGATGAG
CACCAGCCCGGCCCCCTCACCTGGTATATGGGCATTCTCACCACCAAAGACGTCAAGGGCATGCTCGCCACCCTGCTGCG
GCCGGGGGATCGCTTCTTCGCCCTGCCCCTCTTCGGCCACGCCGGAACCCATCCGCAAGAACTGGCCCACCTGGCCCAGA
TCTTGCAGCCCCATCTTCGCTCTGTCCAGGCGCTGCCGGATCTTGCCGCTTTACAAGCCCAACTGCAGCAGCCGGCTGAC
CTCGACGAGCCGCCCGCGGTTATCTGTGGATCCCTATACCTCTTGGGCCAGGTGATGCAAGCTTGCCTGGGATGGGATCT
GGCCTGCTGA

Upstream 100 bases:

>100_bases
GGCATTCGCGACGCTTTTGTGGTGAACCTTTGACAGCTTTTGGCGCCAGTGAGGAAGTGCCGAGTTGAGGTGAAGCTTGA
GCCTGAATACCCTGTCGCCA

Downstream 100 bases:

>100_bases
GGTTCCTGCCACCAGTTTGGCCGAGCGCTCGGAGACCCCTAGCGCTTCCCCTACCGGCCCCATCGGGATGACAGGATTTG
AACCTGCGGCCCTCTGCTCC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MGGISTWDWPGFLESLGHFGVDLSLDRMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWVSHALWAAGYRVGRYTSPHLV
NWRERIWLSGEYISPGDWSRVLAQVRDCLQTYAPGEASPTQFEAITAAAWLYFRQQKVDIAVMEVGLGGRLDATNAGIEP
VVTAITSIGWDHWQRLGNSLAQIAFEKAGILKPGIPTVCAPQPPEVMAVIRARAEQTGSPLRVVEPLHWAGPQTVCWRDQ
VLTLPLAGEVQLINGAVALAVVETLQEQGWRIPDVAVEKGFALTRWPGRLQWVQIGSRQLLLDGAHNLPAAQALRRYLDE
HQPGPLTWYMGILTTKDVKGMLATLLRPGDRFFALPLFGHAGTHPQELAHLAQILQPHLRSVQALPDLAALQAQLQQPAD
LDEPPAVICGSLYLLGQVMQACLGWDLAC

Sequences:

>Translated_429_residues
MGGISTWDWPGFLESLGHFGVDLSLDRMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWVSHALWAAGYRVGRYTSPHLV
NWRERIWLSGEYISPGDWSRVLAQVRDCLQTYAPGEASPTQFEAITAAAWLYFRQQKVDIAVMEVGLGGRLDATNAGIEP
VVTAITSIGWDHWQRLGNSLAQIAFEKAGILKPGIPTVCAPQPPEVMAVIRARAEQTGSPLRVVEPLHWAGPQTVCWRDQ
VLTLPLAGEVQLINGAVALAVVETLQEQGWRIPDVAVEKGFALTRWPGRLQWVQIGSRQLLLDGAHNLPAAQALRRYLDE
HQPGPLTWYMGILTTKDVKGMLATLLRPGDRFFALPLFGHAGTHPQELAHLAQILQPHLRSVQALPDLAALQAQLQQPAD
LDEPPAVICGSLYLLGQVMQACLGWDLAC
>Mature_428_residues
GGISTWDWPGFLESLGHFGVDLSLDRMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWVSHALWAAGYRVGRYTSPHLVN
WRERIWLSGEYISPGDWSRVLAQVRDCLQTYAPGEASPTQFEAITAAAWLYFRQQKVDIAVMEVGLGGRLDATNAGIEPV
VTAITSIGWDHWQRLGNSLAQIAFEKAGILKPGIPTVCAPQPPEVMAVIRARAEQTGSPLRVVEPLHWAGPQTVCWRDQV
LTLPLAGEVQLINGAVALAVVETLQEQGWRIPDVAVEKGFALTRWPGRLQWVQIGSRQLLLDGAHNLPAAQALRRYLDEH
QPGPLTWYMGILTTKDVKGMLATLLRPGDRFFALPLFGHAGTHPQELAHLAQILQPHLRSVQALPDLAALQAQLQQPADL
DEPPAVICGSLYLLGQVMQACLGWDLAC

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=343, Percent_Identity=35.5685131195335, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI66932984, Length=343, Percent_Identity=35.5685131195335, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI1788654, Length=419, Percent_Identity=33.1742243436754, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71984923, Length=284, Percent_Identity=34.8591549295775, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=284, Percent_Identity=34.8591549295775, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17553150, Length=284, Percent_Identity=34.8591549295775, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6323760, Length=424, Percent_Identity=33.4905660377358, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324815, Length=287, Percent_Identity=32.0557491289199, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6322718, Length=424, Percent_Identity=28.5377358490566, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24641571, Length=323, Percent_Identity=29.4117647058824, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24581568, Length=331, Percent_Identity=27.7945619335347, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46865; Mature: 46733

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGISTWDWPGFLESLGHFGVDLSLDRMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWV
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHH
SHALWAAGYRVGRYTSPHLVNWRERIWLSGEYISPGDWSRVLAQVRDCLQTYAPGEASPT
HHHHHHHHHHCCCCCCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
QFEAITAAAWLYFRQQKVDIAVMEVGLGGRLDATNAGIEPVVTAITSIGWDHWQRLGNSL
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
AQIAFEKAGILKPGIPTVCAPQPPEVMAVIRARAEQTGSPLRVVEPLHWAGPQTVCWRDQ
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCC
VLTLPLAGEVQLINGAVALAVVETLQEQGWRIPDVAVEKGFALTRWPGRLQWVQIGSRQL
EEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCEEEEEECCCEE
LLDGAHNLPAAQALRRYLDEHQPGPLTWYMGILTTKDVKGMLATLLRPGDRFFALPLFGH
EECCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
AGTHPQELAHLAQILQPHLRSVQALPDLAALQAQLQQPADLDEPPAVICGSLYLLGQVMQ
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ACLGWDLAC
HHHCCCCCC
>Mature Secondary Structure 
GGISTWDWPGFLESLGHFGVDLSLDRMQVLLQRLGQPQAGIPAIHVAGTNGKGSVCAWV
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHH
SHALWAAGYRVGRYTSPHLVNWRERIWLSGEYISPGDWSRVLAQVRDCLQTYAPGEASPT
HHHHHHHHHHCCCCCCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
QFEAITAAAWLYFRQQKVDIAVMEVGLGGRLDATNAGIEPVVTAITSIGWDHWQRLGNSL
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
AQIAFEKAGILKPGIPTVCAPQPPEVMAVIRARAEQTGSPLRVVEPLHWAGPQTVCWRDQ
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCC
VLTLPLAGEVQLINGAVALAVVETLQEQGWRIPDVAVEKGFALTRWPGRLQWVQIGSRQL
EEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCEEEEEECCCEE
LLDGAHNLPAAQALRRYLDEHQPGPLTWYMGILTTKDVKGMLATLLRPGDRFFALPLFGH
EECCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
AGTHPQELAHLAQILQPHLRSVQALPDLAALQAQLQQPADLDEPPAVICGSLYLLGQVMQ
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ACLGWDLAC
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]