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The map label for this gene is truB

Identifier: 86606014

GI number: 86606014

Start: 1351048

End: 1351947

Strand: Reverse

Name: truB

Synonym: CYA_1338

Alternate gene names: 86606014

Gene position: 1351947-1351048 (Counterclockwise)

Preceding gene: 86606016

Following gene: 86606013

Centisome position: 46.1

GC content: 65.0

Gene sequence:

>900_bases
ATGGGATCCCTGGAGGGCTTTCTCAACCTGGATAAGCCGGCCGGGCTCACCTCCCACGACTGTGTGGCGGTGGTGCGGCG
GGTCTTGCGCACCCGCCAGGTGGGGCATGGCGGCACTTTGGATCCGGCGGCAACGGGAGTTCTGCCCATTGCCGTGGGGC
GGGCGACGCGCTTTTTGGCGTTTTTGTCGGGGGACAAGGTCTATCGGGCCACCTTTCGCTTTGGGCAGCGCAGCGACACC
GACGATGCCGAGGGGCAGATCCAGGTGGGATCCCCTTGTCCGGAGCTGAGCCGCGAGCAGGTGGAGGAATGTCTGCGGGA
GTTTGTCGGCGAGATCTGGCAGGTGCCGCCTCTTTACAGCGCCATCAAGCGGCAGGGGAAAAAGCTCTACGAGCTGGCCC
GGGCCGGGGCAGAGGCGGCAGAACTGGGGCTGGAGCCGCGGAGAGTAGTGATTCGACACCTGCAGATCTTGGGCTGGCGC
TCTGGCGAGTTCCCGGAAGTGGATCTGGAGATCGCCTGTGGCCCCGGCACCTACATCCGCGCCCTCGCCCGCGACGTGGG
GGAGAAATTGGGCTGCGGAGGCTTGATGAGCCGCCTGCGACGGCTGCAAAGTGGCCCTTTTCGCAGCGAAGAGAGCCTGC
CCCTGGAGCAGTGGCAGGGATCCGCCGATCCCGCTGCTGCCCTGTTGCCGATTGAGTGGGGATTTCGCAGTTGGAACTCA
GTATTCTTATCTCCGGCTGAGGCGCAGCGCTGGCGACAAGGTCAGGCCATTCCTCTGGATCCGGCTCAGGCCACTGCCGA
GACCCTGCGGGTCATGAGCGGAGAGGGAGAATTTTTGGGCTTGGGGCACAGCCAAGCAGGTCAGCTCAAGCCGCTGCGGG
TGTTGCTGCCGGATCCCTAG

Upstream 100 bases:

>100_bases
GCTTCCAACTCGCAGACAGAAATCAACCCTATGTTAAGCGCTGCGGCAGTTCGGTTACAAATCTTAATTACTGATCAATG
ACTGATGGGTGATGGGCAGC

Downstream 100 bases:

>100_bases
AGCGTTGCAGAAAAGTTACATTTTCGTAACAACATCTGCCATTGGCTGAGGAGAAGGCGGGAAAGAACCCCCCGCATGCT
AAACTTCTGGGGGCAAGCGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MGSLEGFLNLDKPAGLTSHDCVAVVRRVLRTRQVGHGGTLDPAATGVLPIAVGRATRFLAFLSGDKVYRATFRFGQRSDT
DDAEGQIQVGSPCPELSREQVEECLREFVGEIWQVPPLYSAIKRQGKKLYELARAGAEAAELGLEPRRVVIRHLQILGWR
SGEFPEVDLEIACGPGTYIRALARDVGEKLGCGGLMSRLRRLQSGPFRSEESLPLEQWQGSADPAAALLPIEWGFRSWNS
VFLSPAEAQRWRQGQAIPLDPAQATAETLRVMSGEGEFLGLGHSQAGQLKPLRVLLPDP

Sequences:

>Translated_299_residues
MGSLEGFLNLDKPAGLTSHDCVAVVRRVLRTRQVGHGGTLDPAATGVLPIAVGRATRFLAFLSGDKVYRATFRFGQRSDT
DDAEGQIQVGSPCPELSREQVEECLREFVGEIWQVPPLYSAIKRQGKKLYELARAGAEAAELGLEPRRVVIRHLQILGWR
SGEFPEVDLEIACGPGTYIRALARDVGEKLGCGGLMSRLRRLQSGPFRSEESLPLEQWQGSADPAAALLPIEWGFRSWNS
VFLSPAEAQRWRQGQAIPLDPAQATAETLRVMSGEGEFLGLGHSQAGQLKPLRVLLPDP
>Mature_298_residues
GSLEGFLNLDKPAGLTSHDCVAVVRRVLRTRQVGHGGTLDPAATGVLPIAVGRATRFLAFLSGDKVYRATFRFGQRSDTD
DAEGQIQVGSPCPELSREQVEECLREFVGEIWQVPPLYSAIKRQGKKLYELARAGAEAAELGLEPRRVVIRHLQILGWRS
GEFPEVDLEIACGPGTYIRALARDVGEKLGCGGLMSRLRRLQSGPFRSEESLPLEQWQGSADPAAALLPIEWGFRSWNSV
FLSPAEAQRWRQGQAIPLDPAQATAETLRVMSGEGEFLGLGHSQAGQLKPLRVLLPDP

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=257, Percent_Identity=33.852140077821, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI215599015, Length=209, Percent_Identity=33.9712918660287, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI4503337, Length=209, Percent_Identity=33.9712918660287, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI2367200, Length=313, Percent_Identity=39.297124600639, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17553978, Length=216, Percent_Identity=30.0925925925926, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=209, Percent_Identity=32.0574162679426, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=146, Percent_Identity=38.3561643835616, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32689; Mature: 32558

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLEGFLNLDKPAGLTSHDCVAVVRRVLRTRQVGHGGTLDPAATGVLPIAVGRATRFLA
CCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHH
FLSGDKVYRATFRFGQRSDTDDAEGQIQVGSPCPELSREQVEECLREFVGEIWQVPPLYS
HHCCCCEEEHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
AIKRQGKKLYELARAGAEAAELGLEPRRVVIRHLQILGWRSGEFPEVDLEIACGPGTYIR
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHH
ALARDVGEKLGCGGLMSRLRRLQSGPFRSEESLPLEQWQGSADPAAALLPIEWGFRSWNS
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCC
VFLSPAEAQRWRQGQAIPLDPAQATAETLRVMSGEGEFLGLGHSQAGQLKPLRVLLPDP
EEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
GSLEGFLNLDKPAGLTSHDCVAVVRRVLRTRQVGHGGTLDPAATGVLPIAVGRATRFLA
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHH
FLSGDKVYRATFRFGQRSDTDDAEGQIQVGSPCPELSREQVEECLREFVGEIWQVPPLYS
HHCCCCEEEHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
AIKRQGKKLYELARAGAEAAELGLEPRRVVIRHLQILGWRSGEFPEVDLEIACGPGTYIR
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHH
ALARDVGEKLGCGGLMSRLRRLQSGPFRSEESLPLEQWQGSADPAAALLPIEWGFRSWNS
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCC
VFLSPAEAQRWRQGQAIPLDPAQATAETLRVMSGEGEFLGLGHSQAGQLKPLRVLLPDP
EEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA