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The map label for this gene is crp [H]

Identifier: 86606007

GI number: 86606007

Start: 1345253

End: 1345906

Strand: Reverse

Name: crp [H]

Synonym: CYA_1331

Alternate gene names: 86606007

Gene position: 1345906-1345253 (Counterclockwise)

Preceding gene: 86606008

Following gene: 86606004

Centisome position: 45.89

GC content: 61.01

Gene sequence:

>654_bases
ATGTTTCTGTTTGAAGGCTTTTCCCCTGAGGTGCTCGAGCCGCTGGCCCGCCAGATGTTGCTGCGCTCCCACCCTGTTAA
CCAGGCCATCCTGCTACAGAACGACTGGGGCAACGCCGTCTATTTCATCCTGGAGGGTTGGGTCAAGATTCGCACTCACG
ATCTGGAGGGCCGGGAGGTTACCCTCACCATTCTTGGCCCAGGGGAGATCTTCGGGGAAATGGCCGCCTTGGACGAGGCG
CCCCGTTCCAGCGATGTGATTGCCCTGACAGCCACCAAGGTCTGCTCTCTGTCGGCCCAGAACTTTCTGGCCCTTTTGGC
TGCCGATGCGCAGGTTGCCATCCGCCTTGCCCAGCTCTTGTCGCGACGTTTACGGCAGATCAACCGTCGCCTGCAATTGC
GGGAGGCCAGCAGCGTCGCTCGCGTCGCCGATATCCTGCTCTTCTTGGCGGAGTCCCGCGGGCGGGTCGGTTGCAAGGGG
GTAGAAATTCCCAATTTGCCCCACCGGGAGCTGAGCAGCTTGAGTGGCCTGGCCCGGGAAACGGTTACCCGTGTCTTGTC
TAAGCTGGAGCAGGAGGAGCTGATCCGCCGGGATCGGCAGCGGGACATCCTCTGCATCCCCCAGATGGATGCCCTGGAAA
AGCTAATTGGCTAA

Upstream 100 bases:

>100_bases
CAGGTGAAGAGTTTTTCTCTGGGAAGAGAGTTGGGCAAGAATCGAGGCGGTGCAGGAGGAGACCCCAGTGAGCGAGGTAA
CGGCTGAAGATTTACGCCTG

Downstream 100 bases:

>100_bases
AGCGAGGGTTGCCACCTGTTGCCAAAAGCCCTCTTGGCATTCTAAAATGATAGTCCCAGAGCCGGGATAGCTCAGTTGGT
AGAGCAGAGGACTGAAAATC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MFLFEGFSPEVLEPLARQMLLRSHPVNQAILLQNDWGNAVYFILEGWVKIRTHDLEGREVTLTILGPGEIFGEMAALDEA
PRSSDVIALTATKVCSLSAQNFLALLAADAQVAIRLAQLLSRRLRQINRRLQLREASSVARVADILLFLAESRGRVGCKG
VEIPNLPHRELSSLSGLARETVTRVLSKLEQEELIRRDRQRDILCIPQMDALEKLIG

Sequences:

>Translated_217_residues
MFLFEGFSPEVLEPLARQMLLRSHPVNQAILLQNDWGNAVYFILEGWVKIRTHDLEGREVTLTILGPGEIFGEMAALDEA
PRSSDVIALTATKVCSLSAQNFLALLAADAQVAIRLAQLLSRRLRQINRRLQLREASSVARVADILLFLAESRGRVGCKG
VEIPNLPHRELSSLSGLARETVTRVLSKLEQEELIRRDRQRDILCIPQMDALEKLIG
>Mature_217_residues
MFLFEGFSPEVLEPLARQMLLRSHPVNQAILLQNDWGNAVYFILEGWVKIRTHDLEGREVTLTILGPGEIFGEMAALDEA
PRSSDVIALTATKVCSLSAQNFLALLAADAQVAIRLAQLLSRRLRQINRRLQLREASSVARVADILLFLAESRGRVGCKG
VEIPNLPHRELSSLSGLARETVTRVLSKLEQEELIRRDRQRDILCIPQMDALEKLIG

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of one or more genes involved in the induction of competence [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=198, Percent_Identity=28.2828282828283, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24288; Mature: 24288

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLFEGFSPEVLEPLARQMLLRSHPVNQAILLQNDWGNAVYFILEGWVKIRTHDLEGREV
CCEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECEEEEEEECCCCCEE
TLTILGPGEIFGEMAALDEAPRSSDVIALTATKVCSLSAQNFLALLAADAQVAIRLAQLL
EEEEECCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRLRQINRRLQLREASSVARVADILLFLAESRGRVGCKGVEIPNLPHRELSSLSGLARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TVTRVLSKLEQEELIRRDRQRDILCIPQMDALEKLIG
HHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHC
>Mature Secondary Structure
MFLFEGFSPEVLEPLARQMLLRSHPVNQAILLQNDWGNAVYFILEGWVKIRTHDLEGREV
CCEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECEEEEEEECCCCCEE
TLTILGPGEIFGEMAALDEAPRSSDVIALTATKVCSLSAQNFLALLAADAQVAIRLAQLL
EEEEECCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRLRQINRRLQLREASSVARVADILLFLAESRGRVGCKGVEIPNLPHRELSSLSGLARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TVTRVLSKLEQEELIRRDRQRDILCIPQMDALEKLIG
HHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542653; 7542800 [H]