Click here to switch to the map view.

The map label for this gene is map-2

Identifier: 86605846

GI number: 86605846

Start: 1181081

End: 1181854

Strand: Reverse

Name: map-2

Synonym: CYA_1158

Alternate gene names: 86605846

Gene position: 1181854-1181081 (Counterclockwise)

Preceding gene: 86605847

Following gene: 86605845

Centisome position: 40.3

GC content: 64.08

Gene sequence:

>774_bases
ATGCAGCTCATTGAGATCAAGTCCCGCCGGGAGGTGGAGAAGATGCGGCGGGCCGGGCGGGTGGTGGCCCAGGTGCTGCA
GGAAATTGCCGAGTGTCTGGAGCCAGGCTGGACAACGGCAGACATCGATGCCTATGCCGAGCGGCGGGTAGCGGAGTTGA
ATGCCCTGCCCAGCTTCAAGGGCTACTACGGCTTTCCCGCCTGTGTCTGTGTCAGCGTCAACCATGAGGTGGTACACGGG
ATCCCCAGCCCCCGCAAAGTGGTGCAGCCGGGGGACATCGTCAAGGTGGATTTTGGCGCCATGGTCGAAGGCTGGCATGC
CGACTCTTGCATCACCATTGGCCTGGAGCCCCTCGGCGATGCCGCCCGCGACCTGATCGACACAGCGGCCAAAGCGCTGC
AGACGGGCATTGACCACGTGCGCCACGGCGTCTGGCTGCAGGATGTCTCAGGCGCCATCGAGGACTACATCCAGGGGCGG
GGCTACTCCGTGGTGAAGCAGTACGTCGGCCACGGCGTGGGGCGCAACTTGCACGAGGAGCCGCAGTTTCCCCACTACCG
CACCCGCGACCTTCCCAATCCCAAGCTGCGCTCCGGCATGACGGTGGCAATCGAGCCGATGGTGAATGCAGGGGGAGAGG
CCACGCGGGTGCTCTCGGATGGCTGGACGGTGGTGACGGTGGACGGCTCTTGGTCGGCCCAGTTTGAGCACACGGTCTTG
ATCACGGACTCTGGGGCTGAGATTTTGACGGATCGCACCCCTTTTCAGTCCTAG

Upstream 100 bases:

>100_bases
CGATGGAATCGGTACAAGAACATCTGCGGGCTCTCTTGGAAGGCTTCAGGAGGACAGCGTGATCAAGACGGGGGCGGGAA
ATCTCTCCCCGCGGCGGGGC

Downstream 100 bases:

>100_bases
GGATCCCTTGCTGAAGGGCGGCAACGGCTAGGATAGAAGAGGTGGAGTATTGTTTCGGCTCTTAACTCGCTCTTAACAGG
GCGGGTTTCCTATCGATATT

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAECLEPGWTTADIDAYAERRVAELNALPSFKGYYGFPACVCVSVNHEVVHG
IPSPRKVVQPGDIVKVDFGAMVEGWHADSCITIGLEPLGDAARDLIDTAAKALQTGIDHVRHGVWLQDVSGAIEDYIQGR
GYSVVKQYVGHGVGRNLHEEPQFPHYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLSDGWTVVTVDGSWSAQFEHTVL
ITDSGAEILTDRTPFQS

Sequences:

>Translated_257_residues
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAECLEPGWTTADIDAYAERRVAELNALPSFKGYYGFPACVCVSVNHEVVHG
IPSPRKVVQPGDIVKVDFGAMVEGWHADSCITIGLEPLGDAARDLIDTAAKALQTGIDHVRHGVWLQDVSGAIEDYIQGR
GYSVVKQYVGHGVGRNLHEEPQFPHYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLSDGWTVVTVDGSWSAQFEHTVL
ITDSGAEILTDRTPFQS
>Mature_257_residues
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAECLEPGWTTADIDAYAERRVAELNALPSFKGYYGFPACVCVSVNHEVVHG
IPSPRKVVQPGDIVKVDFGAMVEGWHADSCITIGLEPLGDAARDLIDTAAKALQTGIDHVRHGVWLQDVSGAIEDYIQGR
GYSVVKQYVGHGVGRNLHEEPQFPHYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLSDGWTVVTVDGSWSAQFEHTVL
ITDSGAEILTDRTPFQS

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=249, Percent_Identity=40.1606425702811, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI40385867, Length=248, Percent_Identity=36.2903225806452, Blast_Score=165, Evalue=4e-41,
Organism=Escherichia coli, GI1786364, Length=250, Percent_Identity=41.6, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1788728, Length=253, Percent_Identity=26.4822134387352, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996291, Length=252, Percent_Identity=36.9047619047619, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6323273, Length=254, Percent_Identity=35.8267716535433, Blast_Score=163, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24583427, Length=246, Percent_Identity=36.9918699186992, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21355531, Length=249, Percent_Identity=36.144578313253, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 28229; Mature: 28229

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAECLEPGWTTADIDAYAERRVAELNALPSFK
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
GYYGFPACVCVSVNHEVVHGIPSPRKVVQPGDIVKVDFGAMVEGWHADSCITIGLEPLGD
CCCCCCEEEEEECCHHHHCCCCCCHHCCCCCCEEEEECCHHHCCCCCCCEEEECCCCCCH
AARDLIDTAAKALQTGIDHVRHGVWLQDVSGAIEDYIQGRGYSVVKQYVGHGVGRNLHEE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
PQFPHYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLSDGWTVVTVDGSWSAQFEHTVL
CCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCCEEEEEE
ITDSGAEILTDRTPFQS
EECCCCCEEECCCCCCC
>Mature Secondary Structure
MQLIEIKSRREVEKMRRAGRVVAQVLQEIAECLEPGWTTADIDAYAERRVAELNALPSFK
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
GYYGFPACVCVSVNHEVVHGIPSPRKVVQPGDIVKVDFGAMVEGWHADSCITIGLEPLGD
CCCCCCEEEEEECCHHHHCCCCCCHHCCCCCCEEEEECCHHHCCCCCCCEEEECCCCCCH
AARDLIDTAAKALQTGIDHVRHGVWLQDVSGAIEDYIQGRGYSVVKQYVGHGVGRNLHEE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
PQFPHYRTRDLPNPKLRSGMTVAIEPMVNAGGEATRVLSDGWTVVTVDGSWSAQFEHTVL
CCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCCEEEEEE
ITDSGAEILTDRTPFQS
EECCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]