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The map label for this gene is ndhF [H]

Identifier: 86605354

GI number: 86605354

Start: 651333

End: 653168

Strand: Reverse

Name: ndhF [H]

Synonym: CYA_0640

Alternate gene names: 86605354

Gene position: 653168-651333 (Counterclockwise)

Preceding gene: 86605355

Following gene: 86605353

Centisome position: 22.27

GC content: 62.2

Gene sequence:

>1836_bases
ATGAGTTTGGACGGGCTGGTCAGAACCGCGTGGTTAATTCCCCTCTATGCGCTAGTGGCGTGGCTGGGGGCGTTGATTTG
GTCCCCTGGGATCACCCGGCGCACCGGCCCCCGACCGGCGGGCTATCTAAATGCGCTGCTGACCTTGGTGGCGTTCTTGC
ACAGTGTGGGGGCGCTGGTGGCGATTTGGGATCAGCCGGCGCAGGTGATTCGGCTGCCCTGGCTGCAGGTAGGGGATCTG
GTGTTGGAGCTGCCGCTGCAGGTTTCATCGCTAACGCTGGGGGCCTGCGCCGTGGTGACGGGGCTAAACCTGGCGGCCCA
GATCTACGGCTTTGGCTACATGGAAATGGACTGGGGCTGGGCGCGGTTCTTCTCGCTGCTGGGCCTGTTTGAGGGGGGGA
TGTGCGCTCTGGCTCTGTGCGACTCTTTGTTTTTCAGCTACGTCATCCTGGAGATTTTGACTCTGGGCACCTACCTGTTG
GTGGGCTTTTGGTTTAACCAATCGCTGGTGGTAACTGGGGCCCGGGATGCTTTTCTAACCAAGCGCATCGGCGACCTGTT
GCTCTTGATGGGGGTCATCGCCATCTATCCTTTGACGGGATCCTGGGAGTTTGAGGACTTGACGGCTTGGGCAAAGTCGG
TGCAGGCAGGAGCTATCTCTGTGGATCCGAACTTGATCGCCTTGGTGGGGCTGGGGTTGCTGGCCGGCCCTGTGGGCAAG
TGTGCCCAGTTCCCTCTGCATCTGTGGCTGGATGAGGCGATGGAAGGGCCGATCCCCTCTACGATCTTGCGCAATGCGGT
GGTGGTGGGCACAGGAGCTTGGGTGTTGATTCGGCTGCAGCCGCTCTATGAGCTTTCCCCGACGGTGTTGCGGCTGATGC
TGGTGATCGGGGGGCTGACGGCAGTGGGGGGATCCCTGGTGGCCCTGGCCCAAATTGATGCCAAGCGGGCCTTGTCCTAC
CTGGTGAGCGCCTACATGGGCTTGATCTTTGTAGCGGTGGGCACAGGACACACGCAACAGGCCTACCAACTGCTGCTCAC
CTATGCGCCGGCGATGGCCTTGCTGGTGATGGCGCTGGGCATGGTGATCTGGAACAGCATTACCCAGGATCTGCGCTATC
TGGGGGGCTTGTGGTCGCGGCGGCCCATTTCCGGCCTGTGCTTTTTGACGGGGATGGCCGGCATGGTGGCCCTGCCGCCT
TTCGGCTCCTTCTGGGTGCTGACGGATCTGTGGCAGCAGTTGGCGCAATCGGGGCAGGGGCTGTGGGCGGCAGTGGTGCT
GGTCACCAACGCGGTAACGGCGCTGGGGCTGACGCGGGTATTCGGCCTGGTTTGGGGAGGCAAGCCGCAGCAGATGAGCG
AGCGCTCCCCAGAGGTGCACTGGCCGATGATGTTGCCCAGCACGGCCATGATGGGGGTGACGCTGCATGTGCCGATGATT
CTGGCGGCCTTGGATCTCTTGCCGGCTCTGCAGGGGATCCCGATTGCGGTGATGGCCGGATCCAGCTTGGCGGGCATCGC
GTTGGGAGCCTGGCTGTACCTGCCGCGGGGGCGGCGGCCAGTGGACTGGGCTTTCTTGGCCGACTGGCAGGATCTGCTGG
CCAACGACTTCTACACCCCCAAGCTCTACCGCGCCAGCGTGGTGTTTGTGGTGCGCATCATTTCTGAGGTTACGGCCCTC
TTTGACCGCTACGTGGTGGACGGCGTGGTGAATTTGACCAGCTTAGTTGTGCTGGCAGGAGGTCAGGTTTTGAAATACAC
CACCTCCGGCCAGTTGCAGGCCTATGTGCTGACGATTGTGGTGGGGGTGGCCCTGCTGGGCTGGCTAATTCTCTAG

Upstream 100 bases:

>100_bases
TTTGTCTATCCTAGTTTGCCACTAGGATGCCTCTTAAGATTTTTTACAGGATCTCAGTAGGCGATGGGGTCAGGCGGGAT
TCCTGGCCGTAAGGAGGATT

Downstream 100 bases:

>100_bases
GCAGAGGGGGACGGAGTCCTTAAGAAAGAGGGAAAAGACCATGCTGAGTGCCTTGATTGTGTTGCCGATTTTGGGATCCC
TGCTGCTGTTCCTGGGCTTG

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSLDGLVRTAWLIPLYALVAWLGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALVAIWDQPAQVIRLPWLQVGDL
VLELPLQVSSLTLGACAVVTGLNLAAQIYGFGYMEMDWGWARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLL
VGFWFNQSLVVTGARDAFLTKRIGDLLLLMGVIAIYPLTGSWEFEDLTAWAKSVQAGAISVDPNLIALVGLGLLAGPVGK
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLRLMLVIGGLTAVGGSLVALAQIDAKRALSY
LVSAYMGLIFVAVGTGHTQQAYQLLLTYAPAMALLVMALGMVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGMVALPP
FGSFWVLTDLWQQLAQSGQGLWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHVPMI
LAALDLLPALQGIPIAVMAGSSLAGIALGAWLYLPRGRRPVDWAFLADWQDLLANDFYTPKLYRASVVFVVRIISEVTAL
FDRYVVDGVVNLTSLVVLAGGQVLKYTTSGQLQAYVLTIVVGVALLGWLIL

Sequences:

>Translated_611_residues
MSLDGLVRTAWLIPLYALVAWLGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALVAIWDQPAQVIRLPWLQVGDL
VLELPLQVSSLTLGACAVVTGLNLAAQIYGFGYMEMDWGWARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLL
VGFWFNQSLVVTGARDAFLTKRIGDLLLLMGVIAIYPLTGSWEFEDLTAWAKSVQAGAISVDPNLIALVGLGLLAGPVGK
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLRLMLVIGGLTAVGGSLVALAQIDAKRALSY
LVSAYMGLIFVAVGTGHTQQAYQLLLTYAPAMALLVMALGMVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGMVALPP
FGSFWVLTDLWQQLAQSGQGLWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHVPMI
LAALDLLPALQGIPIAVMAGSSLAGIALGAWLYLPRGRRPVDWAFLADWQDLLANDFYTPKLYRASVVFVVRIISEVTAL
FDRYVVDGVVNLTSLVVLAGGQVLKYTTSGQLQAYVLTIVVGVALLGWLIL
>Mature_610_residues
SLDGLVRTAWLIPLYALVAWLGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALVAIWDQPAQVIRLPWLQVGDLV
LELPLQVSSLTLGACAVVTGLNLAAQIYGFGYMEMDWGWARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLLV
GFWFNQSLVVTGARDAFLTKRIGDLLLLMGVIAIYPLTGSWEFEDLTAWAKSVQAGAISVDPNLIALVGLGLLAGPVGKC
AQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLRLMLVIGGLTAVGGSLVALAQIDAKRALSYL
VSAYMGLIFVAVGTGHTQQAYQLLLTYAPAMALLVMALGMVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGMVALPPF
GSFWVLTDLWQQLAQSGQGLWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHVPMIL
AALDLLPALQGIPIAVMAGSSLAGIALGAWLYLPRGRRPVDWAFLADWQDLLANDFYTPKLYRASVVFVVRIISEVTALF
DRYVVDGVVNLTSLVVLAGGQVLKYTTSGQLQAYVLTIVVGVALLGWLIL

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=359, Percent_Identity=29.2479108635098, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1788614, Length=566, Percent_Identity=28.7985865724382, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788831, Length=395, Percent_Identity=26.5822784810127, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788827, Length=443, Percent_Identity=25.0564334085779, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788829, Length=469, Percent_Identity=24.9466950959488, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788613, Length=343, Percent_Identity=25.0728862973761, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 65901; Mature: 65770

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDGLVRTAWLIPLYALVAWLGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEE
AIWDQPAQVIRLPWLQVGDLVLELPLQVSSLTLGACAVVTGLNLAAQIYGFGYMEMDWGW
EEECCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
ARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLLVGFWFNQSLVVTGARDAFLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHH
KRIGDLLLLMGVIAIYPLTGSWEFEDLTAWAKSVQAGAISVDPNLIALVGLGLLAGPVGK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCHHH
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLRLMLVIGGLT
HHHCHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEEECHHHHCCHHHHHHHHHHHHHH
AVGGSLVALAQIDAKRALSYLVSAYMGLIFVAVGTGHTQQAYQLLLTYAPAMALLVMALG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGMVALPPFGSFWVLTDLWQQLAQSGQG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHCCCC
LWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHVPMI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHH
LAALDLLPALQGIPIAVMAGSSLAGIALGAWLYLPRGRRPVDWAFLADWQDLLANDFYTP
HHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
KLYRASVVFVVRIISEVTALFDRYVVDGVVNLTSLVVLAGGQVLKYTTSGQLQAYVLTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
VGVALLGWLIL
HHHHHHHHHHC
>Mature Secondary Structure 
SLDGLVRTAWLIPLYALVAWLGALIWSPGITRRTGPRPAGYLNALLTLVAFLHSVGALV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEE
AIWDQPAQVIRLPWLQVGDLVLELPLQVSSLTLGACAVVTGLNLAAQIYGFGYMEMDWGW
EEECCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
ARFFSLLGLFEGGMCALALCDSLFFSYVILEILTLGTYLLVGFWFNQSLVVTGARDAFLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHH
KRIGDLLLLMGVIAIYPLTGSWEFEDLTAWAKSVQAGAISVDPNLIALVGLGLLAGPVGK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCHHH
CAQFPLHLWLDEAMEGPIPSTILRNAVVVGTGAWVLIRLQPLYELSPTVLRLMLVIGGLT
HHHCHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEEECHHHHCCHHHHHHHHHHHHHH
AVGGSLVALAQIDAKRALSYLVSAYMGLIFVAVGTGHTQQAYQLLLTYAPAMALLVMALG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MVIWNSITQDLRYLGGLWSRRPISGLCFLTGMAGMVALPPFGSFWVLTDLWQQLAQSGQG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHCCCC
LWAAVVLVTNAVTALGLTRVFGLVWGGKPQQMSERSPEVHWPMMLPSTAMMGVTLHVPMI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHH
LAALDLLPALQGIPIAVMAGSSLAGIALGAWLYLPRGRRPVDWAFLADWQDLLANDFYTP
HHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
KLYRASVVFVVRIISEVTALFDRYVVDGVVNLTSLVVLAGGQVLKYTTSGQLQAYVLTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
VGVALLGWLIL
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]