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The map label for this gene is hisIE

Identifier: 86605221

GI number: 86605221

Start: 520709

End: 521404

Strand: Reverse

Name: hisIE

Synonym: CYA_0504

Alternate gene names: 86605221

Gene position: 521404-520709 (Counterclockwise)

Preceding gene: 86605222

Following gene: 86605220

Centisome position: 17.78

GC content: 62.21

Gene sequence:

>696_bases
ATGGTTGTTGCTTTGGCCTCTCTTGCTTCAGCAAATCTGCCCCGCACTCTTCTGCCGGCGGAAATCGCCCATCTAGACAA
CCTCAGCTACAACGAACAGGGTTTGATCCCGGCCATCGTGCAGGACTACCTGGACGGGACGGTGCTGATGATGGCCTGGA
TGAACCGCGAGTCGCTGCAGAAGACTCTGGAAACGGGCCGCACCTGGTTCTGGAGCCGCTCCCGCCAGGCGCTGTGGCCC
AAGGGGGAAACTTCTGGCCACGTGCAGTGGGTCAAGCAAATCCGCTACGACTGCGACAGCGACGCGCTGGTTATCTTGGT
GGAGCAGGTGGGAGAGGCGGCCTGCCACACAGGGGCGCGAAGCTGCTTCTTCCGCACTCTCCCCGAGAAGGAAGGGGATC
CCATGCCGGCCGCCGATACCCTGTCGCAGGTGTTTGCCGTGGTCAAGGCCCGCCAGGCGGATCCCCGGCCCGATTCCTAC
ACCAGCAGCCTGCTGGCCAAGGGAGACAACGCCATCCTGAAAAAACTCGGCGAGGAGACGGCTGAAGTGGTGATGGCGGC
AAAAGAGGGAGACAAGGCAGCCCTGGCCCGCGAGGTGGCGGATCTCTGGTACCACTGCCTCGTGGCTCTTGCTCATCATC
AGGTGGATATTCGGGATGTCTATCGCCAGCTCCAAGCCCGCCGTTCCCCTCAGTGA

Upstream 100 bases:

>100_bases
GGGTTGGGATCTGGGGGGAGTGAGAGATATCCTTTCTGGGGCGAGTTCTGCTATCCTGCTTTCAGGAATCTTTAACCTTT
CTGATTTTCTGCCGCCTTTG

Downstream 100 bases:

>100_bases
AGGTGCTCTGTCCGAGGAGGGGGAGCTGCCTTTGGAGGAGATGGAGCAACCCCCCGCCAAGACCTACCTAGATTTACAGG
CTGCGCGACGGGGCCAGGTG

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MVVALASLASANLPRTLLPAEIAHLDNLSYNEQGLIPAIVQDYLDGTVLMMAWMNRESLQKTLETGRTWFWSRSRQALWP
KGETSGHVQWVKQIRYDCDSDALVILVEQVGEAACHTGARSCFFRTLPEKEGDPMPAADTLSQVFAVVKARQADPRPDSY
TSSLLAKGDNAILKKLGEETAEVVMAAKEGDKAALAREVADLWYHCLVALAHHQVDIRDVYRQLQARRSPQ

Sequences:

>Translated_231_residues
MVVALASLASANLPRTLLPAEIAHLDNLSYNEQGLIPAIVQDYLDGTVLMMAWMNRESLQKTLETGRTWFWSRSRQALWP
KGETSGHVQWVKQIRYDCDSDALVILVEQVGEAACHTGARSCFFRTLPEKEGDPMPAADTLSQVFAVVKARQADPRPDSY
TSSLLAKGDNAILKKLGEETAEVVMAAKEGDKAALAREVADLWYHCLVALAHHQVDIRDVYRQLQARRSPQ
>Mature_231_residues
MVVALASLASANLPRTLLPAEIAHLDNLSYNEQGLIPAIVQDYLDGTVLMMAWMNRESLQKTLETGRTWFWSRSRQALWP
KGETSGHVQWVKQIRYDCDSDALVILVEQVGEAACHTGARSCFFRTLPEKEGDPMPAADTLSQVFAVVKARQADPRPDSY
TSSLLAKGDNAILKKLGEETAEVVMAAKEGDKAALAREVADLWYHCLVALAHHQVDIRDVYRQLQARRSPQ

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=195, Percent_Identity=42.0512820512821, Blast_Score=141, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319819, Length=194, Percent_Identity=35.5670103092784, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH [H]

EC number: =3.5.4.19; =3.6.1.31 [H]

Molecular weight: Translated: 25702; Mature: 25702

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVALASLASANLPRTLLPAEIAHLDNLSYNEQGLIPAIVQDYLDGTVLMMAWMNRESLQ
CEEEHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHH
KTLETGRTWFWSRSRQALWPKGETSGHVQWVKQIRYDCDSDALVILVEQVGEAACHTGAR
HHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
SCFFRTLPEKEGDPMPAADTLSQVFAVVKARQADPRPDSYTSSLLAKGDNAILKKLGEET
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
AEVVMAAKEGDKAALAREVADLWYHCLVALAHHQVDIRDVYRQLQARRSPQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVVALASLASANLPRTLLPAEIAHLDNLSYNEQGLIPAIVQDYLDGTVLMMAWMNRESLQ
CEEEHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHH
KTLETGRTWFWSRSRQALWPKGETSGHVQWVKQIRYDCDSDALVILVEQVGEAACHTGAR
HHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
SCFFRTLPEKEGDPMPAADTLSQVFAVVKARQADPRPDSYTSSLLAKGDNAILKKLGEET
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
AEVVMAAKEGDKAALAREVADLWYHCLVALAHHQVDIRDVYRQLQARRSPQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]