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The map label for this gene is def

Identifier: 86604850

GI number: 86604850

Start: 115188

End: 115784

Strand: Reverse

Name: def

Synonym: CYA_0120

Alternate gene names: 86604850

Gene position: 115784-115188 (Counterclockwise)

Preceding gene: 86604851

Following gene: 86604848

Centisome position: 3.95

GC content: 60.64

Gene sequence:

>597_bases
ATGGTGGCCACCCTTTCTGTCCCCAAAGCCAAGCTGAAAAACCCGCCTCTGCGCATCCATCGCATGGGTGACAAGGTGCT
GCGGCAGCCGGCCAAGCGCGTCAGCCAGGTCAACGACGAGATCCGCCAGTTGGCGCGACACATGCTGCAGACGATGTACA
GCGCCGATGGCATTGGCCTGGCAGCTCCCCAGGTGGGCATTCCCAAGCGCTTGATTGTGGTAGATCTCTATCCCGATAAG
CCGGAAGTGCCACCGCTGGTGCTGATCAACCCGGAGATCCGCGAGTACCTCGGCGAAGTTGTAGCCGGCCAGGAAGGCTG
CCTTAGCATTCCGGGCGTCTTCTGCGAGGTCATGCGGCCGCAGGGGGTGGTGGTTTCGTTTAAGGACGAAACCGGGCGGC
CCCGCACCCTCCAAGCCGACGACCTGCTGGCGCGGGTTATCCAACACGAAATCGATCACCTCAACGGTGTGCTGTTTGTG
GATCACGTGGAAAACGAGCTGCTGTTGGATCAGGAGCTGCGCAAGCACGGCTTCCAGATGCAAGATGTGCAGCGCAAAAA
CAGCACACTTGCCACCCCTCCACCGAAACCTCGCTAG

Upstream 100 bases:

>100_bases
GCTGAACAGCAGACGCATCGCTTCGGCACGGCTTGGACTGAATGTAGAATGGAAGTCCAGACGGGATGTCTGCTGTTGTT
TATTCTGAGAGACCTGCCGG

Downstream 100 bases:

>100_bases
GTCATTGCCGCAGGCGCTGCGCCAAGAGCTGTTCTTCTAGAGCAGCAATGCGGTTGTAGGCAGCGGTTAGCTGGGCGCTC
AGGCGCTGGATCTGGGCTTC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MVATLSVPKAKLKNPPLRIHRMGDKVLRQPAKRVSQVNDEIRQLARHMLQTMYSADGIGLAAPQVGIPKRLIVVDLYPDK
PEVPPLVLINPEIREYLGEVVAGQEGCLSIPGVFCEVMRPQGVVVSFKDETGRPRTLQADDLLARVIQHEIDHLNGVLFV
DHVENELLLDQELRKHGFQMQDVQRKNSTLATPPPKPR

Sequences:

>Translated_198_residues
MVATLSVPKAKLKNPPLRIHRMGDKVLRQPAKRVSQVNDEIRQLARHMLQTMYSADGIGLAAPQVGIPKRLIVVDLYPDK
PEVPPLVLINPEIREYLGEVVAGQEGCLSIPGVFCEVMRPQGVVVSFKDETGRPRTLQADDLLARVIQHEIDHLNGVLFV
DHVENELLLDQELRKHGFQMQDVQRKNSTLATPPPKPR
>Mature_198_residues
MVATLSVPKAKLKNPPLRIHRMGDKVLRQPAKRVSQVNDEIRQLARHMLQTMYSADGIGLAAPQVGIPKRLIVVDLYPDK
PEVPPLVLINPEIREYLGEVVAGQEGCLSIPGVFCEVMRPQGVVVSFKDETGRPRTLQADDLLARVIQHEIDHLNGVLFV
DHVENELLLDQELRKHGFQMQDVQRKNSTLATPPPKPR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=176, Percent_Identity=32.3863636363636, Blast_Score=88, Evalue=4e-18,
Organism=Escherichia coli, GI1789682, Length=147, Percent_Identity=46.9387755102041, Blast_Score=117, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24645728, Length=182, Percent_Identity=34.0659340659341, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645726, Length=161, Percent_Identity=31.6770186335404, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22205; Mature: 22205

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVATLSVPKAKLKNPPLRIHRMGDKVLRQPAKRVSQVNDEIRQLARHMLQTMYSADGIGL
CCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AAPQVGIPKRLIVVDLYPDKPEVPPLVLINPEIREYLGEVVAGQEGCLSIPGVFCEVMRP
CCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
QGVVVSFKDETGRPRTLQADDLLARVIQHEIDHLNGVLFVDHVENELLLDQELRKHGFQM
CCEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCH
QDVQRKNSTLATPPPKPR
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVATLSVPKAKLKNPPLRIHRMGDKVLRQPAKRVSQVNDEIRQLARHMLQTMYSADGIGL
CCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AAPQVGIPKRLIVVDLYPDKPEVPPLVLINPEIREYLGEVVAGQEGCLSIPGVFCEVMRP
CCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
QGVVVSFKDETGRPRTLQADDLLARVIQHEIDHLNGVLFVDHVENELLLDQELRKHGFQM
CCEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCH
QDVQRKNSTLATPPPKPR
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]