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The map label for this gene is nodW [H]

Identifier: 86361277

GI number: 86361277

Start: 612391

End: 613047

Strand: Reverse

Name: nodW [H]

Synonym: RHE_PF00548

Alternate gene names: 86361277

Gene position: 613047-612391 (Counterclockwise)

Preceding gene: 86361278

Following gene: 86361276

Centisome position: 95.41

GC content: 60.43

Gene sequence:

>657_bases
ATGCCTGATATGCGCAAAGCAGCCAAGGAACCAGCCGCGTCCGTCGTCTTCATCGTCGACGACGATATCTCGATGAGAGA
GGCGTTGACCGACCTGTTCCGCTCGATGAAATTCGGCGCTGAAGCGTTCGAAAGCACCTCCGCCTTTCTTGAAAAGGCCG
ATCTGAGCCGACCAGGCTGCCTCCTCCTCGACGTGCAGCTTCCCGGCGTCAGCGGCCTTGATTTTCAGGTCGAGCTGGAG
CGCATCGGCAACCAGATGCCGATCGTCTTCATGACGGGCTTCGGCGACATTCCGATGAGCGTGCGGGCAATGAAGGCCGG
CGCCGTCGACTTTCTGACCAAGCCGTTCGAGGAGCAGGACATTCTCGATGCCGTGGCGACCGCGATGGAGCGCGATGCCG
TACGGCGCCAGGAGAGCGCGCAGAGCGCGGCTGTCACCTCACTTGCCGCAAGCCTGACGCCGCGCGAACGCGAAGTCATG
GATGCCGTCGTCAGGGGGCTGATGAACAAGCAGATCGCTTTCGAACTGGGCATTAGCGAAGTGACGGTAAAACTCCATCG
AGGCAATGTCATGCGCAAGATGGAAGTCCGGTCTGTCGCCGATCTGGTGCGCAAAGCTGAAATCCTCGGCGACCGACACC
GGCCAGCTGTATCTTAG

Upstream 100 bases:

>100_bases
ACCACGCTCGGGGCCCGTGGTGGAAAGCTCGAAGGCGGCAACCATCCCGAGGGTGGAGCGGTCTTTGAAATGTCGCTGCC
GATCAGACCGGAGATGGAAT

Downstream 100 bases:

>100_bases
TATGGTATCTTTCAAGGGGGCATGGGCGCATAAAGGCGGCATAGAGAACAAAAAAGGCTGCCTCATTGCCCTCCGTTCCC
ACCATCGCCGTCGTTGACGA

Product: two-component response regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPDMRKAAKEPAASVVFIVDDDISMREALTDLFRSMKFGAEAFESTSAFLEKADLSRPGCLLLDVQLPGVSGLDFQVELE
RIGNQMPIVFMTGFGDIPMSVRAMKAGAVDFLTKPFEEQDILDAVATAMERDAVRRQESAQSAAVTSLAASLTPREREVM
DAVVRGLMNKQIAFELGISEVTVKLHRGNVMRKMEVRSVADLVRKAEILGDRHRPAVS

Sequences:

>Translated_218_residues
MPDMRKAAKEPAASVVFIVDDDISMREALTDLFRSMKFGAEAFESTSAFLEKADLSRPGCLLLDVQLPGVSGLDFQVELE
RIGNQMPIVFMTGFGDIPMSVRAMKAGAVDFLTKPFEEQDILDAVATAMERDAVRRQESAQSAAVTSLAASLTPREREVM
DAVVRGLMNKQIAFELGISEVTVKLHRGNVMRKMEVRSVADLVRKAEILGDRHRPAVS
>Mature_217_residues
PDMRKAAKEPAASVVFIVDDDISMREALTDLFRSMKFGAEAFESTSAFLEKADLSRPGCLLLDVQLPGVSGLDFQVELER
IGNQMPIVFMTGFGDIPMSVRAMKAGAVDFLTKPFEEQDILDAVATAMERDAVRRQESAQSAAVTSLAASLTPREREVMD
AVVRGLMNKQIAFELGISEVTVKLHRGNVMRKMEVRSVADLVRKAEILGDRHRPAVS

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786747, Length=160, Percent_Identity=27.5, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=33.59375, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788521, Length=194, Percent_Identity=34.020618556701, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790102, Length=144, Percent_Identity=29.8611111111111, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23960; Mature: 23829

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDMRKAAKEPAASVVFIVDDDISMREALTDLFRSMKFGAEAFESTSAFLEKADLSRPGC
CCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LLLDVQLPGVSGLDFQVELERIGNQMPIVFMTGFGDIPMSVRAMKAGAVDFLTKPFEEQD
EEEEEECCCCCCCCCEEEHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHH
ILDAVATAMERDAVRRQESAQSAAVTSLAASLTPREREVMDAVVRGLMNKQIAFELGISE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECHHH
VTVKLHRGNVMRKMEVRSVADLVRKAEILGDRHRPAVS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PDMRKAAKEPAASVVFIVDDDISMREALTDLFRSMKFGAEAFESTSAFLEKADLSRPGC
CCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LLLDVQLPGVSGLDFQVELERIGNQMPIVFMTGFGDIPMSVRAMKAGAVDFLTKPFEEQD
EEEEEECCCCCCCCCEEEHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHH
ILDAVATAMERDAVRRQESAQSAAVTSLAASLTPREREVMDAVVRGLMNKQIAFELGISE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECHHH
VTVKLHRGNVMRKMEVRSVADLVRKAEILGDRHRPAVS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]