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The map label for this gene is fixJ [H]

Identifier: 86361276

GI number: 86361276

Start: 611951

End: 612325

Strand: Reverse

Name: fixJ [H]

Synonym: RHE_PF00547

Alternate gene names: 86361276

Gene position: 612325-611951 (Counterclockwise)

Preceding gene: 86361277

Following gene: 86361268

Centisome position: 95.3

GC content: 61.87

Gene sequence:

>375_bases
TTGCCCTCCGTTCCCACCATCGCCGTCGTTGACGACGACCAGGCCATCCGCGAAGCGATGGATGACCTGATCAAGAACTT
CGGTTACCAGAGCCGGCTGTTCTCCTGTGCGGAAGAGTTTCTCGATTTCGAGCCACGCGCCGAGATCGACTGCATGTTGG
TGGACGTCCAGATGCCGGGCCTTACCGGCCTTGAATTGTTGGCTGAGCTCAACCGCCGTGGGGACGGCCCGCCGATGATC
TTCGTGACCTCCTACGACGATGAGCGCACGCGCAACGCTGCGCTCAATGGCGGCGCCTTCGCCTTTCTCCGCAAACCGGT
CGACGTCAGCACGCTGATCGGATGTCTCGAGGCTGCCTTCAGCCAACGGCCGTGA

Upstream 100 bases:

>100_bases
TCCTCGGCGACCGACACCGGCCAGCTGTATCTTAGTATGGTATCTTTCAAGGGGGCATGGGCGCATAAAGGCGGCATAGA
GAACAAAAAAGGCTGCCTCA

Downstream 100 bases:

>100_bases
GCCCGATTCGCTGCCCGCCAGATCCCAGTCGCTCATCAGATCCTGAAAGGCATCATCGCGCCGCCGGCTCCTGCGATGGC
CGAACTCAACCACGGTTGAG

Product: putative two-component response regulator protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MPSVPTIAVVDDDQAIREAMDDLIKNFGYQSRLFSCAEEFLDFEPRAEIDCMLVDVQMPGLTGLELLAELNRRGDGPPMI
FVTSYDDERTRNAALNGGAFAFLRKPVDVSTLIGCLEAAFSQRP

Sequences:

>Translated_124_residues
MPSVPTIAVVDDDQAIREAMDDLIKNFGYQSRLFSCAEEFLDFEPRAEIDCMLVDVQMPGLTGLELLAELNRRGDGPPMI
FVTSYDDERTRNAALNGGAFAFLRKPVDVSTLIGCLEAAFSQRP
>Mature_123_residues
PSVPTIAVVDDDQAIREAMDDLIKNFGYQSRLFSCAEEFLDFEPRAEIDCMLVDVQMPGLTGLELLAELNRRGDGPPMIF
VTSYDDERTRNAALNGGAFAFLRKPVDVSTLIGCLEAAFSQRP

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=31.8965517241379, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13719; Mature: 13588

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVPTIAVVDDDQAIREAMDDLIKNFGYQSRLFSCAEEFLDFEPRAEIDCMLVDVQMPG
CCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
LTGLELLAELNRRGDGPPMIFVTSYDDERTRNAALNGGAFAFLRKPVDVSTLIGCLEAAF
CHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
SQRP
CCCC
>Mature Secondary Structure 
PSVPTIAVVDDDQAIREAMDDLIKNFGYQSRLFSCAEEFLDFEPRAEIDCMLVDVQMPG
CCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
LTGLELLAELNRRGDGPPMIFVTSYDDERTRNAALNGGAFAFLRKPVDVSTLIGCLEAAF
CHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
SQRP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]