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The map label for this gene is 86361268

Identifier: 86361268

GI number: 86361268

Start: 604549

End: 605847

Strand: Reverse

Name: 86361268

Synonym: RHE_PF00539

Alternate gene names: NA

Gene position: 605847-604549 (Counterclockwise)

Preceding gene: 86361276

Following gene: 86361267

Centisome position: 94.29

GC content: 65.05

Gene sequence:

>1299_bases
ATGCCGCTGGTGCCGACTTTCGCGCCAAAGGCACTGGGGAGCTATTGCACCAAGGTGTTGCCGATACCTTTGGATCGGTC
GGGGCCGGGAACACCAAGGTGCAATGGACCGGTGAGGGCAAGACGGATATCACCCGCGAGCGCAACGCTTCGAATGGAGT
CCGATATGACAGCCTGGCACGACCGTCGCATTCTTGATCTTTTCGGGATTGAACTTCCCATCATCCAGGCGCCGATGGCC
GGTGCGACCACGGTCGACATGGTGATAGCGGCTTCCAGGGCCGGTGGTCTCGGCTCGCTGCCGAGCGCGCAATATTCCGT
CGAGCAGCTGCGGGAGGTGCTTCGGCGCATCAACCAGGAGACGGCCGCTCCCATCAACGTAAACTTCTTCAGCCATATGA
CGCCGGCAGACGATCCGGCCGCCCAGATGAAATGGCGCTCGGCCCTCGCGCCATATTACCGGGAGCTCGGGATCGATCCC
GCCGCTCCCGTCTCCGGTTCGGGCCGGGCGCCCTTCGACGATGCGTTCTGCGATGTCGTCGAAGAGTTTCGTCCAGCCGT
CGTCAGTTTCCATTTCGGCCTTCCGGAGCCGCGGCTGGTCGAGCGCGTGAGGGCCACCGGCGCCAGGATCATATCCTCGG
CGACCATTGTGGAGGAGGCGGTCTGGCTGGAAGCCAACGGTGTCGACGCGGTGATCGCCATGGGCTTCGAGGCCGGCGGC
CACCGCGGCAATTTTCTTACGAAGGACATGGCCACGCAGGTCGGGACGATGGCTCTCGTCCCGCAGATCGTCGATGCGGT
GCAAGTTCCCGTCATCGCCGCCGGCGGTATCGCCGACGGTCGGGGTGTGGCGGCAGCATGGATGCTCGGCGCGTCAGCAG
TTCAGATCGGCACGGCCTATCTCTTCAGCGCCGAAGCGAAGATAGCGGCCGTGCACGCGGCAGCGCTTGGCCACGCAGGC
GATGCCAGCACGGCGATCACCAACCTCTTCACCGGGCGGCCGGCACGCGGCGTCGTCAACCGGATCATGCGCGAACTCGG
GCCGATTTCCGATGTCGCGCCGCCGTTTCCGACGGCAGGCGCTGCCCTTGCGGCCATCCGGGCCAAAGCGGAGGGCAGGG
GGAGCGACGATTTCACCAACCTGTGGGCGGGACAGTCGGCCAGGCTCGCGAAACATCTGCCGGCCGGCGAGCTCACGAGG
TCGCTCTTTGACGAGGCCATCGCGGCGCTTGATGCGCATTCGAAAGCCCGCAAAACGCAAATAGCGGTTCCGGTTTTTCC
GCCGCCGGCGACACCATAG

Upstream 100 bases:

>100_bases
TGCGCGCGAGGCCCCGTCACCAGGTGGGATGAACCGGGCGTCTCACGGGTCGGAAATCTGTCTGAGCGGGCTTGGCCGGA
GGGTCCGGCCGTTGATCTTC

Downstream 100 bases:

>100_bases
TGCAATGGAACCCATCGTCATTCATGCGCAAGATAGGCGCGTCGAAGGCACGCAATACCACATCTGCTAGCTTCGCCAGC
GACCGGAAAGGCGCCGCACG

Product: dioxygenase protein

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPLVPTFAPKALGSYCTKVLPIPLDRSGPGTPRCNGPVRARRISPASATLRMESDMTAWHDRRILDLFGIELPIIQAPMA
GATTVDMVIAASRAGGLGSLPSAQYSVEQLREVLRRINQETAAPINVNFFSHMTPADDPAAQMKWRSALAPYYRELGIDP
AAPVSGSGRAPFDDAFCDVVEEFRPAVVSFHFGLPEPRLVERVRATGARIISSATIVEEAVWLEANGVDAVIAMGFEAGG
HRGNFLTKDMATQVGTMALVPQIVDAVQVPVIAAGGIADGRGVAAAWMLGASAVQIGTAYLFSAEAKIAAVHAAALGHAG
DASTAITNLFTGRPARGVVNRIMRELGPISDVAPPFPTAGAALAAIRAKAEGRGSDDFTNLWAGQSARLAKHLPAGELTR
SLFDEAIAALDAHSKARKTQIAVPVFPPPATP

Sequences:

>Translated_432_residues
MPLVPTFAPKALGSYCTKVLPIPLDRSGPGTPRCNGPVRARRISPASATLRMESDMTAWHDRRILDLFGIELPIIQAPMA
GATTVDMVIAASRAGGLGSLPSAQYSVEQLREVLRRINQETAAPINVNFFSHMTPADDPAAQMKWRSALAPYYRELGIDP
AAPVSGSGRAPFDDAFCDVVEEFRPAVVSFHFGLPEPRLVERVRATGARIISSATIVEEAVWLEANGVDAVIAMGFEAGG
HRGNFLTKDMATQVGTMALVPQIVDAVQVPVIAAGGIADGRGVAAAWMLGASAVQIGTAYLFSAEAKIAAVHAAALGHAG
DASTAITNLFTGRPARGVVNRIMRELGPISDVAPPFPTAGAALAAIRAKAEGRGSDDFTNLWAGQSARLAKHLPAGELTR
SLFDEAIAALDAHSKARKTQIAVPVFPPPATP
>Mature_431_residues
PLVPTFAPKALGSYCTKVLPIPLDRSGPGTPRCNGPVRARRISPASATLRMESDMTAWHDRRILDLFGIELPIIQAPMAG
ATTVDMVIAASRAGGLGSLPSAQYSVEQLREVLRRINQETAAPINVNFFSHMTPADDPAAQMKWRSALAPYYRELGIDPA
APVSGSGRAPFDDAFCDVVEEFRPAVVSFHFGLPEPRLVERVRATGARIISSATIVEEAVWLEANGVDAVIAMGFEAGGH
RGNFLTKDMATQVGTMALVPQIVDAVQVPVIAAGGIADGRGVAAAWMLGASAVQIGTAYLFSAEAKIAAVHAAALGHAGD
ASTAITNLFTGRPARGVVNRIMRELGPISDVAPPFPTAGAALAAIRAKAEGRGSDDFTNLWAGQSARLAKHLPAGELTRS
LFDEAIAALDAHSKARKTQIAVPVFPPPATP

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=361, Percent_Identity=29.3628808864266, Blast_Score=141, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 45357; Mature: 45226

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVPTFAPKALGSYCTKVLPIPLDRSGPGTPRCNGPVRARRISPASATLRMESDMTAWH
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCHHEEEECCCCHHHH
DRRILDLFGIELPIIQAPMAGATTVDMVIAASRAGGLGSLPSAQYSVEQLREVLRRINQE
CCHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TAAPINVNFFSHMTPADDPAAQMKWRSALAPYYRELGIDPAAPVSGSGRAPFDDAFCDVV
HCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
EEFRPAVVSFHFGLPEPRLVERVRATGARIISSATIVEEAVWLEANGVDAVIAMGFEAGG
HHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCC
HRGNFLTKDMATQVGTMALVPQIVDAVQVPVIAAGGIADGRGVAAAWMLGASAVQIGTAY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LFSAEAKIAAVHAAALGHAGDASTAITNLFTGRPARGVVNRIMRELGPISDVAPPFPTAG
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
AALAAIRAKAEGRGSDDFTNLWAGQSARLAKHLPAGELTRSLFDEAIAALDAHSKARKTQ
HHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCE
IAVPVFPPPATP
EEEECCCCCCCC
>Mature Secondary Structure 
PLVPTFAPKALGSYCTKVLPIPLDRSGPGTPRCNGPVRARRISPASATLRMESDMTAWH
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCHHEEEECCCCHHHH
DRRILDLFGIELPIIQAPMAGATTVDMVIAASRAGGLGSLPSAQYSVEQLREVLRRINQE
CCHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TAAPINVNFFSHMTPADDPAAQMKWRSALAPYYRELGIDPAAPVSGSGRAPFDDAFCDVV
HCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
EEFRPAVVSFHFGLPEPRLVERVRATGARIISSATIVEEAVWLEANGVDAVIAMGFEAGG
HHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCC
HRGNFLTKDMATQVGTMALVPQIVDAVQVPVIAAGGIADGRGVAAAWMLGASAVQIGTAY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LFSAEAKIAAVHAAALGHAGDASTAITNLFTGRPARGVVNRIMRELGPISDVAPPFPTAG
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
AALAAIRAKAEGRGSDDFTNLWAGQSARLAKHLPAGELTRSLFDEAIAALDAHSKARKTQ
HHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCE
IAVPVFPPPATP
EEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA