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The map label for this gene is nirK

Identifier: 86361254

GI number: 86361254

Start: 590443

End: 591588

Strand: Direct

Name: nirK

Synonym: RHE_PF00525

Alternate gene names: 86361254

Gene position: 590443-591588 (Clockwise)

Preceding gene: 86361252

Following gene: 86361255

Centisome position: 91.9

GC content: 59.86

Gene sequence:

>1146_bases
ATGACAAATACTCTGCAAATAACCCGGCGCACGATGCTGACGGGTGCTGCTGTTGCCGGAGCGCTGACGCCAATCATCGT
ATCAAGCGCGGCGCAGGCCAATGAAGAGGTTGCAGCTCTCAAGACTGCCAAACAGGTGAAGGTCGACTTGTCGAGGCTTC
CCCGCGAAAAGGTCGAGCTCGTCAAGCCGCCCTTCGTCCATGCTCATCAGCAGAAGGCCGAAGGTGGGCCGAAGATCAAG
GAATTCACGCTAACCATCCAGGAAAAGAAGATGATCCTGGACAAGGACGGCACCGAGATCAACGCCATGACCTTCGACGG
ATCGGTGCCGGGTCCTCTGATGGTGGTGCATCAGGACGACTATGTCGAGCTGACCCTGGTCAACCCCGATAGCAATACGC
TTCAGCACAATATCGACTTCCACGCCGCCACGGGCGGGTTGGGCGGCGGCGCATTGACGGTCGTTAATCCGGGCGAGAGC
ACCGTTCTTCGCTTCAAGGCGACCAAGGCCGGCGTCTTCGTCTATCACTGCGCCCCTCCCGGCATGGTGCCGTGGCACGT
CACATCGGGCATGAACGGCGCCATCATGGTCCTGCCGCGTGAAGGTCTGAAGGACGGCAAGGGCAAGCCGCTCGTCTACG
ACAAGGTCTATTATGTCGGCGAACAGGACTTTTATGTTCCGCGCGACGAAACGGGCAAATTCAAGAAATACGACAGCCCT
GGCGAGGCCTACGATGAGACGGTCGCCGCCATGAAGACGCTAACGCCGACCCATATCGTCTTCAACGGCGCGGTCGGAGC
CCTAACCGGCGAGAATGCGATGACGGCCAAGGTCGGCGAAACGGTGCTGATCGTTCATTCTCAGGCCAACCGCGACACCC
GGCCGCATCTGATCGGCGGGCATGGCGAATATGTCTGGGCGACCGGCAAATTCCTCAACCCACCGGACACGGATCAGGAA
ACCTGGTTCATTCCCGGCGGCACGGCGGGTGCGGCCCTTTATACTTTCGCTCAGCCTGGCATCTATGCCTACGTGAACCA
CAACCTTATCGAGGCCTTCGAGCTGGGGGCGGCGGCCCACTTTAAGGTGACCGGCGAATGGGACGACGATCTCATGACCT
CGGTCAGGGCACCGTCGGCAAGCTGA

Upstream 100 bases:

>100_bases
CAGCCAGTTTGCGCCACGTCAAAGATTTCCGTCGGCTTTCTTCTACTCTCCCCTTCGACAGGGCGGCGTCCGCTCAGTCT
TCGACTACAGGAGATTAGAA

Downstream 100 bases:

>100_bases
CCGGGCGGAGGGGGCGGTGCGCGGGGTCAGGGGCGACCCGCGCACCATTCTCATTTTGGGGGCGCTATTTGCGAAAATAT
CATGGCCCGGAACGCAGCGA

Product: nitrite reductase protein

Products: NA

Alternate protein names: Cu-NIR [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTNTLQITRRTMLTGAAVAGALTPIIVSSAAQANEEVAALKTAKQVKVDLSRLPREKVELVKPPFVHAHQQKAEGGPKIK
EFTLTIQEKKMILDKDGTEINAMTFDGSVPGPLMVVHQDDYVELTLVNPDSNTLQHNIDFHAATGGLGGGALTVVNPGES
TVLRFKATKAGVFVYHCAPPGMVPWHVTSGMNGAIMVLPREGLKDGKGKPLVYDKVYYVGEQDFYVPRDETGKFKKYDSP
GEAYDETVAAMKTLTPTHIVFNGAVGALTGENAMTAKVGETVLIVHSQANRDTRPHLIGGHGEYVWATGKFLNPPDTDQE
TWFIPGGTAGAALYTFAQPGIYAYVNHNLIEAFELGAAAHFKVTGEWDDDLMTSVRAPSAS

Sequences:

>Translated_381_residues
MTNTLQITRRTMLTGAAVAGALTPIIVSSAAQANEEVAALKTAKQVKVDLSRLPREKVELVKPPFVHAHQQKAEGGPKIK
EFTLTIQEKKMILDKDGTEINAMTFDGSVPGPLMVVHQDDYVELTLVNPDSNTLQHNIDFHAATGGLGGGALTVVNPGES
TVLRFKATKAGVFVYHCAPPGMVPWHVTSGMNGAIMVLPREGLKDGKGKPLVYDKVYYVGEQDFYVPRDETGKFKKYDSP
GEAYDETVAAMKTLTPTHIVFNGAVGALTGENAMTAKVGETVLIVHSQANRDTRPHLIGGHGEYVWATGKFLNPPDTDQE
TWFIPGGTAGAALYTFAQPGIYAYVNHNLIEAFELGAAAHFKVTGEWDDDLMTSVRAPSAS
>Mature_380_residues
TNTLQITRRTMLTGAAVAGALTPIIVSSAAQANEEVAALKTAKQVKVDLSRLPREKVELVKPPFVHAHQQKAEGGPKIKE
FTLTIQEKKMILDKDGTEINAMTFDGSVPGPLMVVHQDDYVELTLVNPDSNTLQHNIDFHAATGGLGGGALTVVNPGEST
VLRFKATKAGVFVYHCAPPGMVPWHVTSGMNGAIMVLPREGLKDGKGKPLVYDKVYYVGEQDFYVPRDETGKFKKYDSPG
EAYDETVAAMKTLTPTHIVFNGAVGALTGENAMTAKVGETVLIVHSQANRDTRPHLIGGHGEYVWATGKFLNPPDTDQET
WFIPGGTAGAALYTFAQPGIYAYVNHNLIEAFELGAAAHFKVTGEWDDDLMTSVRAPSAS

Specific function: Unknown

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 plastocyanin-like domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011707
- InterPro:   IPR008972
- InterPro:   IPR001287
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07732 Cu-oxidase_3 [H]

EC number: =1.7.2.1 [H]

Molecular weight: Translated: 41032; Mature: 40901

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTLQITRRTMLTGAAVAGALTPIIVSSAAQANEEVAALKTAKQVKVDLSRLPREKVEL
CCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHH
VKPPFVHAHQQKAEGGPKIKEFTLTIQEKKMILDKDGTEINAMTFDGSVPGPLMVVHQDD
CCCCCCCCHHHHCCCCCCEEEEEEEEEECEEEEECCCCEEEEEEECCCCCCCEEEEECCC
YVELTLVNPDSNTLQHNIDFHAATGGLGGGALTVVNPGESTVLRFKATKAGVFVYHCAPP
EEEEEEECCCCCCEEEECEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCC
GMVPWHVTSGMNGAIMVLPREGLKDGKGKPLVYDKVYYVGEQDFYVPRDETGKFKKYDSP
CCCCEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCCC
GEAYDETVAAMKTLTPTHIVFNGAVGALTGENAMTAKVGETVLIVHSQANRDTRPHLIGG
CHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCEEEECCCEEEEEEECCCCCCCCCEECC
HGEYVWATGKFLNPPDTDQETWFIPGGTAGAALYTFAQPGIYAYVNHNLIEAFELGAAAH
CCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCHHHHHHCCCEEE
FKVTGEWDDDLMTSVRAPSAS
EEEECCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TNTLQITRRTMLTGAAVAGALTPIIVSSAAQANEEVAALKTAKQVKVDLSRLPREKVEL
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHH
VKPPFVHAHQQKAEGGPKIKEFTLTIQEKKMILDKDGTEINAMTFDGSVPGPLMVVHQDD
CCCCCCCCHHHHCCCCCCEEEEEEEEEECEEEEECCCCEEEEEEECCCCCCCEEEEECCC
YVELTLVNPDSNTLQHNIDFHAATGGLGGGALTVVNPGESTVLRFKATKAGVFVYHCAPP
EEEEEEECCCCCCEEEECEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCC
GMVPWHVTSGMNGAIMVLPREGLKDGKGKPLVYDKVYYVGEQDFYVPRDETGKFKKYDSP
CCCCEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCCC
GEAYDETVAAMKTLTPTHIVFNGAVGALTGENAMTAKVGETVLIVHSQANRDTRPHLIGG
CHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCEEEECCCEEEEEEECCCCCCCCCEECC
HGEYVWATGKFLNPPDTDQETWFIPGGTAGAALYTFAQPGIYAYVNHNLIEAFELGAAAH
CCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCHHHHHHCCCEEE
FKVTGEWDDDLMTSVRAPSAS
EEEECCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899992 [H]