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The map label for this gene is purR [H]

Identifier: 86361035

GI number: 86361035

Start: 343261

End: 344268

Strand: Direct

Name: purR [H]

Synonym: RHE_PF00305

Alternate gene names: 86361035

Gene position: 343261-344268 (Clockwise)

Preceding gene: 86361028

Following gene: 86361036

Centisome position: 53.42

GC content: 65.08

Gene sequence:

>1008_bases
ATGACGACCATAAGAGATGTCGCGCGCCTTGCGGGAGTTTCAATCTCGACCGTCTCGCTTGCGCTCAACAGCCCGAAGCG
GGTGGGCGCCGAGACGCTGGACCGCATTCAGCAGGCGATCAAATCGACCGGCTACCGCATCGATCCGGTGGCCCAGACGC
TGGCGCGCGGGCGCAGCTCGCTCGTCGGTTTCGTGTCCGCCAATCTCGGCAACATGTTCTTCGGCGACATCCGCCGCGAG
ATCGAGCACCAGGCGCTCGATCATGGCTATTTCGTCCTGATCGCCGACTCTTCCGGCAGGGCCGATCTCGAACGGGCGCT
GTTGGAGCGAATGGAAGCCCAGAAGATCGCCGGCATCGCGCTGGCGACCAACGGCCACGGCGAGGAATACGCCGCGTTCC
TGCGCGACTTCAAGACGCCGATCGTGATGTTCGACCAGAAGGTGGAAGGTGCCGAGCGCGATTTTGTCGGCTCCGACAAT
CCGCTGACGACGACCATCCTGACCGAGCACCTGCTGCAGCTCGGCCACCGGCGCATCGCCTTCATCTCCGGCCCAAGCGG
CCTGCACACCGCCGACGAGCGTCTGAAGGGCTTCATCGATACGATGGCCGCCGCCGGCGTCGATGTCGATCCCTCGCTGG
TCACCGAGGGCGGCTATACCAGGACCGGCGGCCATACGCAGGCGATGCGGCTGCTCACCCTCCGCGACAGGCCGACCGCC
ATCATCGGCGCCAACAACATGATGGGGCTCGCGACGCTGCAGGTGATGCAGGAAATGGGCTTCCGCTGCCCTGAGGACGT
ATCGCTGGCAATGGTCGACGATGTGCCCTGGAGCAACGTCATCACGCCGCGCATAACCATGGTGGTGCAGGATGCCCAGA
AGCTCGGCGAACTGGCGGCGCAGCGGCTGCTGGCAAGAATCTCAAGCCCCGAAGCCGCCGCCGAGCCGCCGCGGGATTTC
ATTCTGACGCCGAGATTCGTGCGGGGGGAGTCGACCAAGCGGCTGTGA

Upstream 100 bases:

>100_bases
CCCAGCGTCGATGACGCAGATTCTGGCCTGTCAAAGGGGACAAGTTGCACAGACGAAGGAAATCTGCCAAAAGGAAAGCC
TTAGAACCGGTTCGATGGAC

Downstream 100 bases:

>100_bases
TTCTATTGCGAAGGCAGCGAGATCGGCTTCTCGCCATCGAGGCTATCGGCCCAGGCCTGCACAAACCGGTGATTGACAGT
ATTACCCCCATCAACGTCCG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTTIRDVARLAGVSISTVSLALNSPKRVGAETLDRIQQAIKSTGYRIDPVAQTLARGRSSLVGFVSANLGNMFFGDIRRE
IEHQALDHGYFVLIADSSGRADLERALLERMEAQKIAGIALATNGHGEEYAAFLRDFKTPIVMFDQKVEGAERDFVGSDN
PLTTTILTEHLLQLGHRRIAFISGPSGLHTADERLKGFIDTMAAAGVDVDPSLVTEGGYTRTGGHTQAMRLLTLRDRPTA
IIGANNMMGLATLQVMQEMGFRCPEDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAAQRLLARISSPEAAAEPPRDF
ILTPRFVRGESTKRL

Sequences:

>Translated_335_residues
MTTIRDVARLAGVSISTVSLALNSPKRVGAETLDRIQQAIKSTGYRIDPVAQTLARGRSSLVGFVSANLGNMFFGDIRRE
IEHQALDHGYFVLIADSSGRADLERALLERMEAQKIAGIALATNGHGEEYAAFLRDFKTPIVMFDQKVEGAERDFVGSDN
PLTTTILTEHLLQLGHRRIAFISGPSGLHTADERLKGFIDTMAAAGVDVDPSLVTEGGYTRTGGHTQAMRLLTLRDRPTA
IIGANNMMGLATLQVMQEMGFRCPEDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAAQRLLARISSPEAAAEPPRDF
ILTPRFVRGESTKRL
>Mature_334_residues
TTIRDVARLAGVSISTVSLALNSPKRVGAETLDRIQQAIKSTGYRIDPVAQTLARGRSSLVGFVSANLGNMFFGDIRREI
EHQALDHGYFVLIADSSGRADLERALLERMEAQKIAGIALATNGHGEEYAAFLRDFKTPIVMFDQKVEGAERDFVGSDNP
LTTTILTEHLLQLGHRRIAFISGPSGLHTADERLKGFIDTMAAAGVDVDPSLVTEGGYTRTGGHTQAMRLLTLRDRPTAI
IGANNMMGLATLQVMQEMGFRCPEDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAAQRLLARISSPEAAAEPPRDFI
LTPRFVRGESTKRL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=29.4642857142857, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790369, Length=308, Percent_Identity=28.8961038961039, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=28.6173633440514, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=32.0689655172414, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=27.4924471299094, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=26.1980830670927, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789068, Length=291, Percent_Identity=26.8041237113402, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786540, Length=318, Percent_Identity=28.9308176100629, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787906, Length=339, Percent_Identity=25.6637168141593, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=25.5591054313099, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=25.4019292604502, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789456, Length=310, Percent_Identity=25.4838709677419, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786268, Length=311, Percent_Identity=23.1511254019293, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790689, Length=312, Percent_Identity=24.0384615384615, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36514; Mature: 36383

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIRDVARLAGVSISTVSLALNSPKRVGAETLDRIQQAIKSTGYRIDPVAQTLARGRSS
CCCHHHHHHHHCCCEEEEEEEECCCHHCCHHHHHHHHHHHHHCCCEECHHHHHHHCCHHH
LVGFVSANLGNMFFGDIRREIEHQALDHGYFVLIADSSGRADLERALLERMEAQKIAGIA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCEE
LATNGHGEEYAAFLRDFKTPIVMFDQKVEGAERDFVGSDNPLTTTILTEHLLQLGHRRIA
EEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
FISGPSGLHTADERLKGFIDTMAAAGVDVDPSLVTEGGYTRTGGHTQAMRLLTLRDRPTA
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHEEEEEECCCCCE
IIGANNMMGLATLQVMQEMGFRCPEDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAA
EEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QRLLARISSPEAAAEPPRDFILTPRFVRGESTKRL
HHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure 
TTIRDVARLAGVSISTVSLALNSPKRVGAETLDRIQQAIKSTGYRIDPVAQTLARGRSS
CCHHHHHHHHCCCEEEEEEEECCCHHCCHHHHHHHHHHHHHCCCEECHHHHHHHCCHHH
LVGFVSANLGNMFFGDIRREIEHQALDHGYFVLIADSSGRADLERALLERMEAQKIAGIA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCEE
LATNGHGEEYAAFLRDFKTPIVMFDQKVEGAERDFVGSDNPLTTTILTEHLLQLGHRRIA
EEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
FISGPSGLHTADERLKGFIDTMAAAGVDVDPSLVTEGGYTRTGGHTQAMRLLTLRDRPTA
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHEEEEEECCCCCE
IIGANNMMGLATLQVMQEMGFRCPEDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAA
EEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QRLLARISSPEAAAEPPRDFILTPRFVRGESTKRL
HHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA