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The map label for this gene is cscA

Identifier: 86360784

GI number: 86360784

Start: 52091

End: 53809

Strand: Direct

Name: cscA

Synonym: RHE_PF00050

Alternate gene names: 86360784

Gene position: 52091-53809 (Clockwise)

Preceding gene: 86360783

Following gene: 86360786

Centisome position: 8.11

GC content: 62.42

Gene sequence:

>1719_bases
ATGACAAGCCAAGCGATATTTCCGTCCGGTGAAGGCGGGCGGGCGACGATCAGCGCCGTCCTGCCGGCGGGAACCACAAT
CCATGCCTGGATCAAGGCCTTGCGTGGCGGCGCCCTGGGAAGGCTGAGCGCTCAAGTCGACGGCGATCCGGCCTGTGCCG
TCGAAACATCAAATCTCTATGAATTCGAGTTTCGGCTGCTGACGCTGGAGCGCGGTGGAGATACCGCCTTGTCCTACGAT
CCCGCTGCGACGAGCGTCTCGCTTGTCTATGCCTTCTCTCAGCCCCGCGTTCTCGAAGAGGGCATCCGGCTGCTGCATAT
CAGGCCTTCGAATGCCGCACCCGACATTACCGGCGGCTATCACTTCCGCCCGCCTTTCGGCTGGATGAACGATCCCAATG
GGTTTGGAAGCTTCGGCGGCAAGGTGCACCTCTTCTATCAGCACTATCCCCATAGCCTTCGTTGGAACAACATGCATTGG
GGTCATGCGGTCTCGGAGGACTACCTTCGCTGGACGCATCTCCCGATCTTTCTTCCGCCTTCGGACGAACTCGCCGCCCG
GGCCGACGGGCTGGGCGGCGCATTTTCCGGCTCCGCGATCGCTCTTCCCGGCGATGAAGCCGGCTTTCGCATCTTCTTCA
CCGAACACATGAAGGACCGGGAACCCGAGGAACAGGTTCAGTTCACAGCCATCAGCCGCGATCTTGTCAACGTCGAACCG
GCAAGCCTGATCCTGCCCACGCGGCCCGCGGGCCTCGGTCTGACGAAGGACTTCCGCGATCCCTATGTCTTCCCGGGCCC
CGACGACAAATGGAAGATGCTCGTGGGCACGCGCGACCGCGAGGGCGGCGTCATCCTGCTCTACGAAACCGATGACCCGG
CTGCGGCGACAGGATGGACCTTCCTCGGCATTCTTCACCGCGAGAACCGCTTCGGCATGACGGCGGCGGAATGCCCTTGC
ATGGTGCCTCTCAACGGTGCCGCAAATGACCCAGCGACATGCTGGGCCCTTATCTTCGGTCTTCTGACCAGCCGCGACCC
GGCCACGGGCCGGCGCAATATCACGCTTGCCACCGTCGGCCGCTTCGATGGCCACAGCTTTTCTGTCGAGTTCGAGCAGG
AGTTGGACTTCGGCACCGACGCCTACGCGTTCCAGGCCTTCGTCGACAAGACCGGTCCGGTCGGCATCGCATGGCTTGCG
AACTGGACGGATGTTTCAAAGGAGGTCGATATGCCGACGGCGATGACGCTGCCGCGGCGGCTGGCATTGCACGGCGGCGC
GCTGATCACGGCGCCGGTCGCCGGTGTCGAAACTCTGCGGCAGCGACAGCTGGATTCTCAAGACCTTCTGAACGGCCGTG
CGGTCAATCTCGCCAACGGCTCTGTCGAGATCCTCCTCACGCTCGAGGAAGCGGGCAACGCCTTCCATCTCGCATTCGAA
CATCCCGAGGCGGCGGTCGCGGTACAGTTGAATGACGAGGGACTGAGCATTCTCTTTTCGGGAGCGGGTGCCAAGAGCTC
GCCCCGTTATATCGCCGCCGGAGCACGCCCGGCAACCATCCGCATATTCCTCGACGCAGGCTCCATCGAGGTCTTCGGTG
ACGATGGCCGCTGGGCGGGCACTAAGCGGCTGCCGGGGTTTAAGGGGATAAATTCCATGCGGCTCGACGCACCAGAGGGA
CATGTGCTCAACGCCAATATCTGGCAACTTGGTCTTTGA

Upstream 100 bases:

>100_bases
CGCCTCCGTTTTGATGATGATCCCGATCCTGGGGCTCTACCTCGTTTTCCAACGCTGGTTCGTGAGTTCCTTTATCGGCT
CTGCCGTGAAAGGTTGAAGG

Downstream 100 bases:

>100_bases
TCTCAGGTGCAATCCTGGCAAACGCACGGCCGCCGGGGCGCCGGCCGTTGCGAAGGATCATGGCGCATTGAGCGGCTAGG
CCGCCTTGCCCTTACCCTTG

Product: beta-fructofuranosidase protein

Products: NA

Alternate protein names: Invertase; Sucrase [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MTSQAIFPSGEGGRATISAVLPAGTTIHAWIKALRGGALGRLSAQVDGDPACAVETSNLYEFEFRLLTLERGGDTALSYD
PAATSVSLVYAFSQPRVLEEGIRLLHIRPSNAAPDITGGYHFRPPFGWMNDPNGFGSFGGKVHLFYQHYPHSLRWNNMHW
GHAVSEDYLRWTHLPIFLPPSDELAARADGLGGAFSGSAIALPGDEAGFRIFFTEHMKDREPEEQVQFTAISRDLVNVEP
ASLILPTRPAGLGLTKDFRDPYVFPGPDDKWKMLVGTRDREGGVILLYETDDPAAATGWTFLGILHRENRFGMTAAECPC
MVPLNGAANDPATCWALIFGLLTSRDPATGRRNITLATVGRFDGHSFSVEFEQELDFGTDAYAFQAFVDKTGPVGIAWLA
NWTDVSKEVDMPTAMTLPRRLALHGGALITAPVAGVETLRQRQLDSQDLLNGRAVNLANGSVEILLTLEEAGNAFHLAFE
HPEAAVAVQLNDEGLSILFSGAGAKSSPRYIAAGARPATIRIFLDAGSIEVFGDDGRWAGTKRLPGFKGINSMRLDAPEG
HVLNANIWQLGL

Sequences:

>Translated_572_residues
MTSQAIFPSGEGGRATISAVLPAGTTIHAWIKALRGGALGRLSAQVDGDPACAVETSNLYEFEFRLLTLERGGDTALSYD
PAATSVSLVYAFSQPRVLEEGIRLLHIRPSNAAPDITGGYHFRPPFGWMNDPNGFGSFGGKVHLFYQHYPHSLRWNNMHW
GHAVSEDYLRWTHLPIFLPPSDELAARADGLGGAFSGSAIALPGDEAGFRIFFTEHMKDREPEEQVQFTAISRDLVNVEP
ASLILPTRPAGLGLTKDFRDPYVFPGPDDKWKMLVGTRDREGGVILLYETDDPAAATGWTFLGILHRENRFGMTAAECPC
MVPLNGAANDPATCWALIFGLLTSRDPATGRRNITLATVGRFDGHSFSVEFEQELDFGTDAYAFQAFVDKTGPVGIAWLA
NWTDVSKEVDMPTAMTLPRRLALHGGALITAPVAGVETLRQRQLDSQDLLNGRAVNLANGSVEILLTLEEAGNAFHLAFE
HPEAAVAVQLNDEGLSILFSGAGAKSSPRYIAAGARPATIRIFLDAGSIEVFGDDGRWAGTKRLPGFKGINSMRLDAPEG
HVLNANIWQLGL
>Mature_571_residues
TSQAIFPSGEGGRATISAVLPAGTTIHAWIKALRGGALGRLSAQVDGDPACAVETSNLYEFEFRLLTLERGGDTALSYDP
AATSVSLVYAFSQPRVLEEGIRLLHIRPSNAAPDITGGYHFRPPFGWMNDPNGFGSFGGKVHLFYQHYPHSLRWNNMHWG
HAVSEDYLRWTHLPIFLPPSDELAARADGLGGAFSGSAIALPGDEAGFRIFFTEHMKDREPEEQVQFTAISRDLVNVEPA
SLILPTRPAGLGLTKDFRDPYVFPGPDDKWKMLVGTRDREGGVILLYETDDPAAATGWTFLGILHRENRFGMTAAECPCM
VPLNGAANDPATCWALIFGLLTSRDPATGRRNITLATVGRFDGHSFSVEFEQELDFGTDAYAFQAFVDKTGPVGIAWLAN
WTDVSKEVDMPTAMTLPRRLALHGGALITAPVAGVETLRQRQLDSQDLLNGRAVNLANGSVEILLTLEEAGNAFHLAFEH
PEAAVAVQLNDEGLSILFSGAGAKSSPRYIAAGARPATIRIFLDAGSIEVFGDDGRWAGTKRLPGFKGINSMRLDAPEGH
VLNANIWQLGL

Specific function: Hydrolysis of sucrose, raffinose, inulin and levan. Specific for the fructose moiety and the beta-anomeric configuration of the glycosidic linkages of its substrates. The enzyme released fructose from sucrose and raffinose, and the fructose polymer inulin

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=329, Percent_Identity=30.3951367781155, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 61969; Mature: 61837

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQAIFPSGEGGRATISAVLPAGTTIHAWIKALRGGALGRLSAQVDGDPACAVETSNLY
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEE
EFEFRLLTLERGGDTALSYDPAATSVSLVYAFSQPRVLEEGIRLLHIRPSNAAPDITGGY
EEEEEEEEEECCCCCEEEECCCCCEEEEEEEECCCHHHHCCCEEEEEECCCCCCCCCCCE
HFRPPFGWMNDPNGFGSFGGKVHLFYQHYPHSLRWNNMHWGHAVSEDYLRWTHLPIFLPP
EECCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEEEECC
SDELAARADGLGGAFSGSAIALPGDEAGFRIFFTEHMKDREPEEQVQFTAISRDLVNVEP
CHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHEEEEEEECHHCCCCC
ASLILPTRPAGLGLTKDFRDPYVFPGPDDKWKMLVGTRDREGGVILLYETDDPAAATGWT
CEEEECCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCEE
FLGILHRENRFGMTAAECPCMVPLNGAANDPATCWALIFGLLTSRDPATGRRNITLATVG
EEEEEEECCCCCCCHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
RFDGHSFSVEFEQELDFGTDAYAFQAFVDKTGPVGIAWLANWTDVSKEVDMPTAMTLPRR
CCCCCEEEEEEHHCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHCCCCHHHHCCHH
LALHGGALITAPVAGVETLRQRQLDSQDLLNGRAVNLANGSVEILLTLEEAGNAFHLAFE
HHHCCCEEEEECHHHHHHHHHHCCCHHHHCCCCEEEEECCCEEEEEEEECCCCEEEEEEE
HPEAAVAVQLNDEGLSILFSGAGAKSSPRYIAAGARPATIRIFLDAGSIEVFGDDGRWAG
CCCEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCCEEEEEEEECCCEEEECCCCCCCC
TKRLPGFKGINSMRLDAPEGHVLNANIWQLGL
CCCCCCCCCCCCEEECCCCCEEEECCEEEECC
>Mature Secondary Structure 
TSQAIFPSGEGGRATISAVLPAGTTIHAWIKALRGGALGRLSAQVDGDPACAVETSNLY
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEE
EFEFRLLTLERGGDTALSYDPAATSVSLVYAFSQPRVLEEGIRLLHIRPSNAAPDITGGY
EEEEEEEEEECCCCCEEEECCCCCEEEEEEEECCCHHHHCCCEEEEEECCCCCCCCCCCE
HFRPPFGWMNDPNGFGSFGGKVHLFYQHYPHSLRWNNMHWGHAVSEDYLRWTHLPIFLPP
EECCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEEEECC
SDELAARADGLGGAFSGSAIALPGDEAGFRIFFTEHMKDREPEEQVQFTAISRDLVNVEP
CHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHEEEEEEECHHCCCCC
ASLILPTRPAGLGLTKDFRDPYVFPGPDDKWKMLVGTRDREGGVILLYETDDPAAATGWT
CEEEECCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCEE
FLGILHRENRFGMTAAECPCMVPLNGAANDPATCWALIFGLLTSRDPATGRRNITLATVG
EEEEEEECCCCCCCHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
RFDGHSFSVEFEQELDFGTDAYAFQAFVDKTGPVGIAWLANWTDVSKEVDMPTAMTLPRR
CCCCCEEEEEEHHCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHCCCCHHHHCCHH
LALHGGALITAPVAGVETLRQRQLDSQDLLNGRAVNLANGSVEILLTLEEAGNAFHLAFE
HHHCCCEEEEECHHHHHHHHHHCCCHHHHCCCCEEEEECCCEEEEEEEECCCCEEEEEEE
HPEAAVAVQLNDEGLSILFSGAGAKSSPRYIAAGARPATIRIFLDAGSIEVFGDDGRWAG
CCCEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCCEEEEEEEECCCEEEECCCCCCCC
TKRLPGFKGINSMRLDAPEGHVLNANIWQLGL
CCCCCCCCCCCCEEECCCCCEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9720201; 10360571 [H]