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The map label for this gene is pyrE [H]

Identifier: 85860423

GI number: 85860423

Start: 2590681

End: 2591271

Strand: Direct

Name: pyrE [H]

Synonym: SYN_02702

Alternate gene names: 85860423

Gene position: 2590681-2591271 (Clockwise)

Preceding gene: 85860422

Following gene: 85860424

Centisome position: 81.49

GC content: 61.42

Gene sequence:

>591_bases
ATGACAACTCAGGAAGCCAGAAAGCAGCGTCTCATTGAAATCATCCTGGAGAAATCCTTCAAATACAGTGAAGATCCGCC
CTTTACGCTGGCCTCGGGAAGAACGAGCCCTTACTATTTCAACTGCAAGCCAACGACCCTGAATCCGGAAGGGATGAACC
TCATCGGCAGCCTGCTGGCGGATATGCTGGCCGACGCGGACGTGACAGCCGCCGGAGGGCTTACCCTGGGAGCCGATCCG
CTGGCCAACGCCCTGGCCGTCATCTCCTGGCAGAAGGGGAAGCCCATCAAGGCCTTCATCGTCCGCAAGGATGTCAAGGG
GCACGGGACCAGGAGCGCCCTGGAAGGTGACGTGCAGCCGGGCGAGCGGGTCGTCATTCTCGATGACGTCATCACCACGG
GAGGCTCCACGATCACCGCCATCGAGCGCGCACGGGAGGCCGGACTGGTCATCGACAGGGTGATTGCCCTGATCGACCGG
GAAGAAGGCGGCCGCGCCAACATCGAAGCCCATGTCCCCCGCGTCGACGCCGTCCTGACCCGGTCGGAGATCATGAATCA
CTTTTCAGGCAAAGCCCCGATCGCGTCCTGA

Upstream 100 bases:

>100_bases
GGCGCTTCGATTTACTATCTCAGCGAATGCGCCCTTTTTCCGGACGCCCCGCTATCCTGTCGAATGCGGCCCGATGTAAA
TTTATTCAAGGAGGTAAATC

Downstream 100 bases:

>100_bases
TCCGGCGCGGCTTATACCGGAAAATCAGGACACCTGATACCAGTTCCGGATCAGATCAGAAAGGATATCCATGCGGCAAG
ACAGAAGCGACGCGGACCAG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MTTQEARKQRLIEIILEKSFKYSEDPPFTLASGRTSPYYFNCKPTTLNPEGMNLIGSLLADMLADADVTAAGGLTLGADP
LANALAVISWQKGKPIKAFIVRKDVKGHGTRSALEGDVQPGERVVILDDVITTGGSTITAIERAREAGLVIDRVIALIDR
EEGGRANIEAHVPRVDAVLTRSEIMNHFSGKAPIAS

Sequences:

>Translated_196_residues
MTTQEARKQRLIEIILEKSFKYSEDPPFTLASGRTSPYYFNCKPTTLNPEGMNLIGSLLADMLADADVTAAGGLTLGADP
LANALAVISWQKGKPIKAFIVRKDVKGHGTRSALEGDVQPGERVVILDDVITTGGSTITAIERAREAGLVIDRVIALIDR
EEGGRANIEAHVPRVDAVLTRSEIMNHFSGKAPIAS
>Mature_195_residues
TTQEARKQRLIEIILEKSFKYSEDPPFTLASGRTSPYYFNCKPTTLNPEGMNLIGSLLADMLADADVTAAGGLTLGADPL
ANALAVISWQKGKPIKAFIVRKDVKGHGTRSALEGDVQPGERVVILDDVITTGGSTITAIERAREAGLVIDRVIALIDRE
EGGRANIEAHVPRVDAVLTRSEIMNHFSGKAPIAS

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=170, Percent_Identity=30, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1790073, Length=199, Percent_Identity=28.643216080402, Blast_Score=64, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17554964, Length=167, Percent_Identity=34.1317365269461, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17508631, Length=161, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17933654, Length=197, Percent_Identity=29.9492385786802, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 21052; Mature: 20921

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQEARKQRLIEIILEKSFKYSEDPPFTLASGRTSPYYFNCKPTTLNPEGMNLIGSLLA
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHH
DMLADADVTAAGGLTLGADPLANALAVISWQKGKPIKAFIVRKDVKGHGTRSALEGDVQP
HHHCCCCCCCCCCCEECCCHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHCCCCCC
GERVVILDDVITTGGSTITAIERAREAGLVIDRVIALIDREEGGRANIEAHVPRVDAVLT
CCEEEEEECHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHH
RSEIMNHFSGKAPIAS
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTQEARKQRLIEIILEKSFKYSEDPPFTLASGRTSPYYFNCKPTTLNPEGMNLIGSLLA
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHH
DMLADADVTAAGGLTLGADPLANALAVISWQKGKPIKAFIVRKDVKGHGTRSALEGDVQP
HHHCCCCCCCCCCCEECCCHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHCCCCCC
GERVVILDDVITTGGSTITAIERAREAGLVIDRVIALIDREEGGRANIEAHVPRVDAVLT
CCEEEEEECHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHH
RSEIMNHFSGKAPIAS
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA