| Definition | Syntrophus aciditrophicus SB chromosome, complete genome. |
|---|---|
| Accession | NC_007759 |
| Length | 3,179,300 |
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The map label for this gene is metH [H]
Identifier: 85860422
GI number: 85860422
Start: 2588061
End: 2590502
Strand: Direct
Name: metH [H]
Synonym: SYN_02703
Alternate gene names: 85860422
Gene position: 2588061-2590502 (Clockwise)
Preceding gene: 85860419
Following gene: 85860423
Centisome position: 81.4
GC content: 63.68
Gene sequence:
>2442_bases ATGAAGTCCAAAGAAACGATCCGCCGCCTTTTAAAGAAGAAGATCCTGGTTCTCGACGGCGCCACGGGAACGGAGCTGCA GAAACGGGGCATGCCCGGCGGGGTCTGCCCGGAACTGTGGTGCCTGGAACATCCGGAGATCATTAAGGAAATCCACAGAT CCTACCGGCAGGCGGGTTCAGACATCGTCTATACCTGCACCTTCGGGGCGAACCGTTACAAACTCGCCCAGTACGGGACG CGGGAAGTCCGGGAAATCAATCGGAACCTGGCCCGGCTCGCCCGGCATGCCGTCGGCCCGAAAGCCCTCGTCGCCGGAGA CATCGGCCCGACCGGCCATTTCGTGGAACCCTTCGGCGATCTCCCCTTCGAAGAGGCCGTCTCCGCCTTCAAGGAACAGA TCCAGGGGCTGCTGGAGGGAGGCGTCGATCTGTTCGTCATCGAAACGATGATGGACATCCAGGAGGCCCGCGCCGCCCTG ATTGCCGTCAAGGAAACCTGCCCGGCCTTTACAGTCGTCACCCTCACCTATGAAAACGGCGGCCGCACCCTCAACGGAAC CGATCCCGCCACGGCCCTGATCACCCTGCAGAGCCTCGGCGCCGATGCCGTGGGCTGCAACTGCTCCACCGGTCCGGAGG CCATGCTGGCCTTCATCGAGGCCATGAAGCCCCACGCCACCGTTCCCCTGGCCGCCAAGCCCAACGCGGGACTGCCCCGG CTCGAGGGGGAAGAGACCTTCTTCGACATGGACGCCGAGACCTTCGCCTCCCACGGTCCGGCCTTCGCCGCCGCCGGGGT CAACCTGCTGGGCGGCTGCTGCGGGACAACCCCTGACCATATCCAGGCCCTGGCCGGGACCCTGCAAAATGCCCCGCCGC GTCCCCCCGTCCGCGCCGCGCTAAGCGCCGTCAGTTCCGCCCGGAGTTACCGTCTGCTGACCCGGAACGAACCTCTGGTC ATCGTCGGCGAACGCCTCAATCCCACGGGGAAGAAGGCCCTTCAGCAGGAGCTGCTGGCGGGAAAGATGTCCCTCGTCCG GCAGATGACCCGGGAACAGGAACAGCAGAAGGCCGACCTTCTGGACGTCAATGTCGGGGTTCCCGGCCTCGATGAAGTCA AAACGATCCGGGAAGTGATTTCCCTGCTGGCCACGGCGACGGAACTGCCGCTGGTCATCGACTCCTCGAAGGTGGAAACC ATCGAGACGGCCCTGCGCCTTTATCCCGGCCGCGCCCTGATCAACTCCATCTCGGGGGAAAAGAAAAAATGCGAACGGCT CCTTCCCCTGGCAGCCCGCTACGGGGCGATGTTCATCCTCCTGCCCCTGGCCGACGGCGAGGTTCCCGAAACAGCCGACC GGCGGATCGCCATTGTCCGGGAGGTCTACGGCAAGGCCCGGCGCTTGGGCTTCACGAAGGAAGACATCGTCGTCGACGGC CTGGTCATGACGGCCGCCACGAATGCCAACGCACCGGCGGAAACCCTGAAGACAATCGCCTGGTGCACCGACCGCTTCCA GGCGAAAACGATCCTGGGCATTTCCAACGTCTCTTTCGGCCTGCCGGAACGCAAATGGCTCAATGCCGCCTTTCTGGCTA TGGCCCAGCTCTCCGGCCTGACGATGGCCATCGCCAACCCGTCCATGGAGGAACTCATGGCCCTCAAGCGGGCGGCGGAC GTGCTGACGGCAAGGGACGCTCAGGCCTCCGCCTACATCGCTCATTTCGCCGGAACCGCCGAAGGGAAAAAGCCGGCGGG AACATCGGCAATGCAGCCCCCGGCCCCCGCGGGTCCGGAAGAAAAAGTCCGCGCCGCCGTGCTGGAAGGCAACCGCGACG ATATCGGCGATCTGGTTGAAGGCGCCCTGAGCGCCGGTTTCGAGGCCCGGAGCATCGTCGATGACCTGCTCATCCCGGCC ATTGTCCAGGTGGGAGAACTTTTTGACCGGAAGGTCTATTTTCTCCCCCAGCTTATCGCCAGCGCCGAGACGATGAAGAA GGCCCTGGCGATCCTGGAGCCCCGGCTGAAGAGCGGCGGCATCCCGGAAAAAGCCAGGGGAACGGTGGTCATGGCAACGG TCCAGGGAGACATCCACGACATCGGAAAGAACATCGTCGTCCTCATGCTGAAAAACCATGGTTTCCGGGTTCTCGATCTG GGCAAGGACGTCCCGGTGGAAACCATTCTCGAAACGATCCGGACGGAAAACCCGGACGTGGTGGGGCTTTCCGCCCTGAT GACCACGACGATGGTCTCCATGAAAACAACCATCGAGCAGGCCCGATCGGCAGACCTGACCTGCCCCTTTCTGATCGGGG GCGCCGTCGTGACCCGGTCATACGCGGATTCCATCGGCGCCGCTTACGCCCGGGACGGCGTGGAAGCGGTCCGTGTCGTC GAACAGCTCATCGGAAGCGCGGGCGGAGAACTTTCGAAATAA
Upstream 100 bases:
>100_bases ACAAAAGGCTATTGACAGGGCCTAACCCTACAGGCTAAAAACAAACCCTCAAAAGATGACATGATCAAGCCATTCGACCT CTACTCTGGAAAGGAACTTC
Downstream 100 bases:
>100_bases CTCCAGCCGGATTACATTGGGTTTTTAACATCCGACATCAGGTCGCATTTTTTTGATAACTGTATCATCTTCTGCATCGG CGCTTCGATTTACTATCTCA
Product: 5-methyltetrahydrofolate--homocysteine methyltransferase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 813; Mature: 813
Protein sequence:
>813_residues MKSKETIRRLLKKKILVLDGATGTELQKRGMPGGVCPELWCLEHPEIIKEIHRSYRQAGSDIVYTCTFGANRYKLAQYGT REVREINRNLARLARHAVGPKALVAGDIGPTGHFVEPFGDLPFEEAVSAFKEQIQGLLEGGVDLFVIETMMDIQEARAAL IAVKETCPAFTVVTLTYENGGRTLNGTDPATALITLQSLGADAVGCNCSTGPEAMLAFIEAMKPHATVPLAAKPNAGLPR LEGEETFFDMDAETFASHGPAFAAAGVNLLGGCCGTTPDHIQALAGTLQNAPPRPPVRAALSAVSSARSYRLLTRNEPLV IVGERLNPTGKKALQQELLAGKMSLVRQMTREQEQQKADLLDVNVGVPGLDEVKTIREVISLLATATELPLVIDSSKVET IETALRLYPGRALINSISGEKKKCERLLPLAARYGAMFILLPLADGEVPETADRRIAIVREVYGKARRLGFTKEDIVVDG LVMTAATNANAPAETLKTIAWCTDRFQAKTILGISNVSFGLPERKWLNAAFLAMAQLSGLTMAIANPSMEELMALKRAAD VLTARDAQASAYIAHFAGTAEGKKPAGTSAMQPPAPAGPEEKVRAAVLEGNRDDIGDLVEGALSAGFEARSIVDDLLIPA IVQVGELFDRKVYFLPQLIASAETMKKALAILEPRLKSGGIPEKARGTVVMATVQGDIHDIGKNIVVLMLKNHGFRVLDL GKDVPVETILETIRTENPDVVGLSALMTTTMVSMKTTIEQARSADLTCPFLIGGAVVTRSYADSIGAAYARDGVEAVRVV EQLIGSAGGELSK
Sequences:
>Translated_813_residues MKSKETIRRLLKKKILVLDGATGTELQKRGMPGGVCPELWCLEHPEIIKEIHRSYRQAGSDIVYTCTFGANRYKLAQYGT REVREINRNLARLARHAVGPKALVAGDIGPTGHFVEPFGDLPFEEAVSAFKEQIQGLLEGGVDLFVIETMMDIQEARAAL IAVKETCPAFTVVTLTYENGGRTLNGTDPATALITLQSLGADAVGCNCSTGPEAMLAFIEAMKPHATVPLAAKPNAGLPR LEGEETFFDMDAETFASHGPAFAAAGVNLLGGCCGTTPDHIQALAGTLQNAPPRPPVRAALSAVSSARSYRLLTRNEPLV IVGERLNPTGKKALQQELLAGKMSLVRQMTREQEQQKADLLDVNVGVPGLDEVKTIREVISLLATATELPLVIDSSKVET IETALRLYPGRALINSISGEKKKCERLLPLAARYGAMFILLPLADGEVPETADRRIAIVREVYGKARRLGFTKEDIVVDG LVMTAATNANAPAETLKTIAWCTDRFQAKTILGISNVSFGLPERKWLNAAFLAMAQLSGLTMAIANPSMEELMALKRAAD VLTARDAQASAYIAHFAGTAEGKKPAGTSAMQPPAPAGPEEKVRAAVLEGNRDDIGDLVEGALSAGFEARSIVDDLLIPA IVQVGELFDRKVYFLPQLIASAETMKKALAILEPRLKSGGIPEKARGTVVMATVQGDIHDIGKNIVVLMLKNHGFRVLDL GKDVPVETILETIRTENPDVVGLSALMTTTMVSMKTTIEQARSADLTCPFLIGGAVVTRSYADSIGAAYARDGVEAVRVV EQLIGSAGGELSK >Mature_813_residues MKSKETIRRLLKKKILVLDGATGTELQKRGMPGGVCPELWCLEHPEIIKEIHRSYRQAGSDIVYTCTFGANRYKLAQYGT REVREINRNLARLARHAVGPKALVAGDIGPTGHFVEPFGDLPFEEAVSAFKEQIQGLLEGGVDLFVIETMMDIQEARAAL IAVKETCPAFTVVTLTYENGGRTLNGTDPATALITLQSLGADAVGCNCSTGPEAMLAFIEAMKPHATVPLAAKPNAGLPR LEGEETFFDMDAETFASHGPAFAAAGVNLLGGCCGTTPDHIQALAGTLQNAPPRPPVRAALSAVSSARSYRLLTRNEPLV IVGERLNPTGKKALQQELLAGKMSLVRQMTREQEQQKADLLDVNVGVPGLDEVKTIREVISLLATATELPLVIDSSKVET IETALRLYPGRALINSISGEKKKCERLLPLAARYGAMFILLPLADGEVPETADRRIAIVREVYGKARRLGFTKEDIVVDG LVMTAATNANAPAETLKTIAWCTDRFQAKTILGISNVSFGLPERKWLNAAFLAMAQLSGLTMAIANPSMEELMALKRAAD VLTARDAQASAYIAHFAGTAEGKKPAGTSAMQPPAPAGPEEKVRAAVLEGNRDDIGDLVEGALSAGFEARSIVDDLLIPA IVQVGELFDRKVYFLPQLIASAETMKKALAILEPRLKSGGIPEKARGTVVMATVQGDIHDIGKNIVVLMLKNHGFRVLDL GKDVPVETILETIRTENPDVVGLSALMTTTMVSMKTTIEQARSADLTCPFLIGGAVVTRSYADSIGAAYARDGVEAVRVV EQLIGSAGGELSK
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=896, Percent_Identity=30.8035714285714, Blast_Score=335, Evalue=1e-91, Organism=Homo sapiens, GI157266337, Length=404, Percent_Identity=26.2376237623762, Blast_Score=91, Evalue=3e-18, Organism=Homo sapiens, GI13162290, Length=322, Percent_Identity=27.639751552795, Blast_Score=87, Evalue=5e-17, Organism=Escherichia coli, GI1790450, Length=853, Percent_Identity=32.1219226260258, Blast_Score=342, Evalue=4e-95, Organism=Escherichia coli, GI1786456, Length=318, Percent_Identity=31.4465408805031, Blast_Score=106, Evalue=7e-24, Organism=Caenorhabditis elegans, GI17535405, Length=856, Percent_Identity=29.9065420560748, Blast_Score=300, Evalue=2e-81, Organism=Saccharomyces cerevisiae, GI6324982, Length=313, Percent_Identity=26.1980830670927, Blast_Score=82, Evalue=3e-16, Organism=Saccharomyces cerevisiae, GI6322966, Length=304, Percent_Identity=23.3552631578947, Blast_Score=76, Evalue=3e-14,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 86985; Mature: 86985
Theoretical pI: Translated: 6.24; Mature: 6.24
Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKSKETIRRLLKKKILVLDGATGTELQKRGMPGGVCPELWCLEHPEIIKEIHRSYRQAGS CCCHHHHHHHHHHHEEEEECCCCCHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCC DIVYTCTFGANRYKLAQYGTREVREINRNLARLARHAVGPKALVAGDIGPTGHFVEPFGD CEEEEEECCCCCEEHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC LPFEEAVSAFKEQIQGLLEGGVDLFVIETMMDIQEARAALIAVKETCPAFTVVTLTYENG CCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC GRTLNGTDPATALITLQSLGADAVGCNCSTGPEAMLAFIEAMKPHATVPLAAKPNAGLPR CCEECCCCCHHHHEEHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC LEGEETFFDMDAETFASHGPAFAAAGVNLLGGCCGTTPDHIQALAGTLQNAPPRPPVRAA CCCCCCEECCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHH LSAVSSARSYRLLTRNEPLVIVGERLNPTGKKALQQELLAGKMSLVRQMTREQEQQKADL HHHHHHHHHEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE LDVNVGVPGLDEVKTIREVISLLATATELPLVIDSSKVETIETALRLYPGRALINSISGE EEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHHCCCC KKKCERLLPLAARYGAMFILLPLADGEVPETADRRIAIVREVYGKARRLGFTKEDIVVDG HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEECC LVMTAATNANAPAETLKTIAWCTDRFQAKTILGISNVSFGLPERKWLNAAFLAMAQLSGL CCEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCC TMAIANPSMEELMALKRAADVLTARDAQASAYIAHFAGTAEGKKPAGTSAMQPPAPAGPE EEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH EKVRAAVLEGNRDDIGDLVEGALSAGFEARSIVDDLLIPAIVQVGELFDRKVYFLPQLIA HHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH SAETMKKALAILEPRLKSGGIPEKARGTVVMATVQGDIHDIGKNIVVLMLKNHGFRVLDL HHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEEECCCEEEEEC GKDVPVETILETIRTENPDVVGLSALMTTTMVSMKTTIEQARSADLTCPFLIGGAVVTRS CCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH YADSIGAAYARDGVEAVRVVEQLIGSAGGELSK HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC >Mature Secondary Structure MKSKETIRRLLKKKILVLDGATGTELQKRGMPGGVCPELWCLEHPEIIKEIHRSYRQAGS CCCHHHHHHHHHHHEEEEECCCCCHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCC DIVYTCTFGANRYKLAQYGTREVREINRNLARLARHAVGPKALVAGDIGPTGHFVEPFGD CEEEEEECCCCCEEHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC LPFEEAVSAFKEQIQGLLEGGVDLFVIETMMDIQEARAALIAVKETCPAFTVVTLTYENG CCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC GRTLNGTDPATALITLQSLGADAVGCNCSTGPEAMLAFIEAMKPHATVPLAAKPNAGLPR CCEECCCCCHHHHEEHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC LEGEETFFDMDAETFASHGPAFAAAGVNLLGGCCGTTPDHIQALAGTLQNAPPRPPVRAA CCCCCCEECCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHH LSAVSSARSYRLLTRNEPLVIVGERLNPTGKKALQQELLAGKMSLVRQMTREQEQQKADL HHHHHHHHHEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE LDVNVGVPGLDEVKTIREVISLLATATELPLVIDSSKVETIETALRLYPGRALINSISGE EEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHHCCCC KKKCERLLPLAARYGAMFILLPLADGEVPETADRRIAIVREVYGKARRLGFTKEDIVVDG HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEECC LVMTAATNANAPAETLKTIAWCTDRFQAKTILGISNVSFGLPERKWLNAAFLAMAQLSGL CCEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCC TMAIANPSMEELMALKRAADVLTARDAQASAYIAHFAGTAEGKKPAGTSAMQPPAPAGPE EEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH EKVRAAVLEGNRDDIGDLVEGALSAGFEARSIVDDLLIPAIVQVGELFDRKVYFLPQLIA HHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH SAETMKKALAILEPRLKSGGIPEKARGTVVMATVQGDIHDIGKNIVVLMLKNHGFRVLDL HHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEEECCCEEEEEC GKDVPVETILETIRTENPDVVGLSALMTTTMVSMKTTIEQARSADLTCPFLIGGAVVTRS CCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH YADSIGAAYARDGVEAVRVVEQLIGSAGGELSK HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9634230 [H]