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The map label for this gene is truA [H]

Identifier: 85860424

GI number: 85860424

Start: 2591342

End: 2592328

Strand: Direct

Name: truA [H]

Synonym: SYN_02701

Alternate gene names: 85860424

Gene position: 2591342-2592328 (Clockwise)

Preceding gene: 85860423

Following gene: 85860426

Centisome position: 81.51

GC content: 61.5

Gene sequence:

>987_bases
ATGCGGCAAGACAGAAGCGACGCGGACCAGCACAGGAAGACCGTCAAGAAAAGAATGACGAAGCCGGAAAGGAAGACAGG
CCCGTCAGCCGGAGAAAGACGGCCGGGAATGAAGCTCCGGCAGGAAAAGGGGGAGAGGCCGCGGCAGAAAGTTCAAGCCG
GTGAAAAGGCCGGCAGGGGGGACGTCTCCCCGGTCAAATACAGGCTCACCCTGGAATATGACGGCACGGGCTACAGCGGC
TGGCAGATGCAGAAGAACGCGAAAAGCATCCAGGGCACCCTGCTCCGCGTTGCCGAGGATCTGCTCGGAGGGCCGGTGGA
CATCCAGGGGGCAGGCCGAACGGACGCCGGCGTCCATGCCCTGGCCCAGGTGGCCCACCTGGAGACGACCCGGAGGATGC
CCCCGCGAAAACTGCTGGAAGGCCTGAACGACGCCCTTCCTTCCAGCATCAACATCCTGCGGGTGGAAGAGGCCCCGCCC
CTTTTTCACGCCCGCCACTCCGCCCAACTGCGCAGCTACCTCTACGTGATTTCCGGCCACCGCACCGCCTTCGGGAAACG
CTACGTCTGGTGGATTCGGGACCACCTGGAACTGAAAAAGATGCAGGCGGCAAGCCGGCTCTTCGCCGGTTTTCATGATT
TTTCTTCTTTTGCCGACAAGCGGATGGACAAGCAGGCCCCCACCCGGGTTAAGGTCGAGGCCACGGAACTGCACGCTTCA
GGCGATCTGATTTTTTTCCGGGTCACCGGTTCCCACTTTCTCTGGAAGATGGTTCGGCGGATGGTGGGGGTCATGGTGGA
GGTGGGACGGGAAAAACTGGATATTTCGGATGTCCGGCAAATGCTGAAGAGTTATTCTCCGGAACCCGCCCGCCTGACGG
CGCCGCCCTCCGGCCTCTACCTGGAGCGGGTCCTTTATGAAGGCGATTCCCTGCAGTCACTCACCCTGCCGCTTGTCCCC
TGGTTCGCCATAGGTCTGCAGAAATAG

Upstream 100 bases:

>100_bases
TTTTCAGGCAAAGCCCCGATCGCGTCCTGATCCGGCGCGGCTTATACCGGAAAATCAGGACACCTGATACCAGTTCCGGA
TCAGATCAGAAAGGATATCC

Downstream 100 bases:

>100_bases
GGCAAAAATGGGCGCTGTCCCTCTATTGACGGCCTATCGGAAAAGCATGACGAAGATTTCGAACAGCTCCGAGCAGATGC
CTGTGCCACGGCATCTCCAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRQDRSDADQHRKTVKKRMTKPERKTGPSAGERRPGMKLRQEKGERPRQKVQAGEKAGRGDVSPVKYRLTLEYDGTGYSG
WQMQKNAKSIQGTLLRVAEDLLGGPVDIQGAGRTDAGVHALAQVAHLETTRRMPPRKLLEGLNDALPSSINILRVEEAPP
LFHARHSAQLRSYLYVISGHRTAFGKRYVWWIRDHLELKKMQAASRLFAGFHDFSSFADKRMDKQAPTRVKVEATELHAS
GDLIFFRVTGSHFLWKMVRRMVGVMVEVGREKLDISDVRQMLKSYSPEPARLTAPPSGLYLERVLYEGDSLQSLTLPLVP
WFAIGLQK

Sequences:

>Translated_328_residues
MRQDRSDADQHRKTVKKRMTKPERKTGPSAGERRPGMKLRQEKGERPRQKVQAGEKAGRGDVSPVKYRLTLEYDGTGYSG
WQMQKNAKSIQGTLLRVAEDLLGGPVDIQGAGRTDAGVHALAQVAHLETTRRMPPRKLLEGLNDALPSSINILRVEEAPP
LFHARHSAQLRSYLYVISGHRTAFGKRYVWWIRDHLELKKMQAASRLFAGFHDFSSFADKRMDKQAPTRVKVEATELHAS
GDLIFFRVTGSHFLWKMVRRMVGVMVEVGREKLDISDVRQMLKSYSPEPARLTAPPSGLYLERVLYEGDSLQSLTLPLVP
WFAIGLQK
>Mature_328_residues
MRQDRSDADQHRKTVKKRMTKPERKTGPSAGERRPGMKLRQEKGERPRQKVQAGEKAGRGDVSPVKYRLTLEYDGTGYSG
WQMQKNAKSIQGTLLRVAEDLLGGPVDIQGAGRTDAGVHALAQVAHLETTRRMPPRKLLEGLNDALPSSINILRVEEAPP
LFHARHSAQLRSYLYVISGHRTAFGKRYVWWIRDHLELKKMQAASRLFAGFHDFSSFADKRMDKQAPTRVKVEATELHAS
GDLIFFRVTGSHFLWKMVRRMVGVMVEVGREKLDISDVRQMLKSYSPEPARLTAPPSGLYLERVLYEGDSLQSLTLPLVP
WFAIGLQK

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=282, Percent_Identity=34.3971631205674, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI70166634, Length=303, Percent_Identity=27.3927392739274, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70166599, Length=303, Percent_Identity=27.3927392739274, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70166645, Length=303, Percent_Identity=27.3927392739274, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=34.156378600823, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71985046, Length=245, Percent_Identity=27.7551020408163, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321112, Length=322, Percent_Identity=25.776397515528, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 37137; Mature: 37137

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQDRSDADQHRKTVKKRMTKPERKTGPSAGERRPGMKLRQEKGERPRQKVQAGEKAGRG
CCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCC
DVSPVKYRLTLEYDGTGYSGWQMQKNAKSIQGTLLRVAEDLLGGPVDIQGAGRTDAGVHA
CCCCEEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
LAQVAHLETTRRMPPRKLLEGLNDALPSSINILRVEEAPPLFHARHSAQLRSYLYVISGH
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECC
RTAFGKRYVWWIRDHLELKKMQAASRLFAGFHDFSSFADKRMDKQAPTRVKVEATELHAS
CHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEECC
GDLIFFRVTGSHFLWKMVRRMVGVMVEVGREKLDISDVRQMLKSYSPEPARLTAPPSGLY
CCEEEEEECCCHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHCCCCCCEEECCCCCHH
LERVLYEGDSLQSLTLPLVPWFAIGLQK
HHHHHHCCCCCCEEHHHHHHHHHHCCCC
>Mature Secondary Structure
MRQDRSDADQHRKTVKKRMTKPERKTGPSAGERRPGMKLRQEKGERPRQKVQAGEKAGRG
CCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCC
DVSPVKYRLTLEYDGTGYSGWQMQKNAKSIQGTLLRVAEDLLGGPVDIQGAGRTDAGVHA
CCCCEEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
LAQVAHLETTRRMPPRKLLEGLNDALPSSINILRVEEAPPLFHARHSAQLRSYLYVISGH
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECC
RTAFGKRYVWWIRDHLELKKMQAASRLFAGFHDFSSFADKRMDKQAPTRVKVEATELHAS
CHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEECC
GDLIFFRVTGSHFLWKMVRRMVGVMVEVGREKLDISDVRQMLKSYSPEPARLTAPPSGLY
CCEEEEEECCCHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHCCCCCCEEECCCCCHH
LERVLYEGDSLQSLTLPLVPWFAIGLQK
HHHHHHCCCCCCEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]