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The map label for this gene is leuD

Identifier: 85860417

GI number: 85860417

Start: 2584489

End: 2584983

Strand: Direct

Name: leuD

Synonym: SYN_00092

Alternate gene names: 85860417

Gene position: 2584489-2584983 (Clockwise)

Preceding gene: 85860416

Following gene: 85860418

Centisome position: 81.29

GC content: 58.79

Gene sequence:

>495_bases
ATGAAATTTACAGGAAAAGTATGGAAATTCGGCGATAACATCGACACGGACGCCATCATCCCGGCCCGTTATCTGAACAC
CTTCGATCCCCAGGCCCTCGCCGCCCACTGCATGGAAGACGCCGATCCCGACTTCCCCAAAAAGGTCAGCGCGGGCGACA
TCATCGTCGCCGGCGAGAACTTCGGCTGCGGCTCATCGAGGGAGCACGCCCCCATCGCCATCAAGGCCGCCGGCGTATCC
TGCGTGATCGCGAAAAGCTTCGCCCGGATCTTCTATCGGAACGCCTTCAACATGGGACTGCCCATTTTCGAATCGGCGGA
ACTGTTTGACCGGGTCGACGAAGGCCAGACCATAACCGTCGACGGCGATTCCGGGGTCATCCTTCTCGAAGGCGCACAGA
CGCCTCTTTCCATCCAGCCGATCCCCCCTTTCATGCAGGAATTGATCGCCGATGGCGGACTCATGAAGCACCTCGCCCGA
AAAAACCGGGGATAA

Upstream 100 bases:

>100_bases
AACTGAAATGAAACTCTCCCGTCTTCCAGAAAACCGTCTCCCCTTTCCGGAGGAGACGGTTTTTTCCGGAAAGACCCAAG
ACTTCTGAAGGATAAAGCGA

Downstream 100 bases:

>100_bases
AAATTTTCCATCCGGCAGCGGCGTGGATTTCAGTGTGAGGTGTAAGGTGTGAGGCGTAAGGCGAGTCTCCATTCGGCAAC
GGCGCGGACTTCGTAAAAGG

Product: aconitase C-terminal domain-containing protein

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKFTGKVWKFGDNIDTDAIIPARYLNTFDPQALAAHCMEDADPDFPKKVSAGDIIVAGENFGCGSSREHAPIAIKAAGVS
CVIAKSFARIFYRNAFNMGLPIFESAELFDRVDEGQTITVDGDSGVILLEGAQTPLSIQPIPPFMQELIADGGLMKHLAR
KNRG

Sequences:

>Translated_164_residues
MKFTGKVWKFGDNIDTDAIIPARYLNTFDPQALAAHCMEDADPDFPKKVSAGDIIVAGENFGCGSSREHAPIAIKAAGVS
CVIAKSFARIFYRNAFNMGLPIFESAELFDRVDEGQTITVDGDSGVILLEGAQTPLSIQPIPPFMQELIADGGLMKHLAR
KNRG
>Mature_164_residues
MKFTGKVWKFGDNIDTDAIIPARYLNTFDPQALAAHCMEDADPDFPKKVSAGDIIVAGENFGCGSSREHAPIAIKAAGVS
CVIAKSFARIFYRNAFNMGLPIFESAELFDRVDEGQTITVDGDSGVILLEGAQTPLSIQPIPPFMQELIADGGLMKHLAR
KNRG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=129, Percent_Identity=41.8604651162791, Blast_Score=80, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=164, Percent_Identity=35.9756097560976, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=161, Percent_Identity=34.1614906832298, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SYNAS (Q2LWJ1)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462619.1
- ProteinModelPortal:   Q2LWJ1
- SMR:   Q2LWJ1
- STRING:   Q2LWJ1
- GeneID:   3883582
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00092
- NMPDR:   fig|56780.10.peg.2564
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   PIFESAE
- BioCyc:   SACI56780:SYN_00092-MONOMER
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17730; Mature: 17730

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTGKVWKFGDNIDTDAIIPARYLNTFDPQALAAHCMEDADPDFPKKVSAGDIIVAGEN
CCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
FGCGSSREHAPIAIKAAGVSCVIAKSFARIFYRNAFNMGLPIFESAELFDRVDEGQTITV
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEE
DGDSGVILLEGAQTPLSIQPIPPFMQELIADGGLMKHLARKNRG
ECCCCEEEEECCCCCEEECCCCHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MKFTGKVWKFGDNIDTDAIIPARYLNTFDPQALAAHCMEDADPDFPKKVSAGDIIVAGEN
CCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
FGCGSSREHAPIAIKAAGVSCVIAKSFARIFYRNAFNMGLPIFESAELFDRVDEGQTITV
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEE
DGDSGVILLEGAQTPLSIQPIPPFMQELIADGGLMKHLARKNRG
ECCCCEEEEECCCCCEEECCCCHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA