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The map label for this gene is leuC

Identifier: 85860416

GI number: 85860416

Start: 2583137

End: 2584399

Strand: Direct

Name: leuC

Synonym: SYN_00091

Alternate gene names: 85860416

Gene position: 2583137-2584399 (Clockwise)

Preceding gene: 85860415

Following gene: 85860417

Centisome position: 81.25

GC content: 61.28

Gene sequence:

>1263_bases
ATGGGAATGACCATTACGGAAAAGATCCTTTGCCGGCATACCGACCTCGACGAAGTCCGTCCCGGAATGCTGATCAACGC
GAAGGTGGATATCGCCCTGGGAAACGACGTCACGGCTCCGCTGGCTATTGACGAATTCCGCAAGGCGGGAGGAAAGAAGG
TCTTCGACCGGGACAAGGTGGTCCTCATTCCGGACCATTTTACCCCCAACAAGGACATCAATTCCGCCGCGCAGTGCAAG
ATCATGCGCGACTTTGCCCGGGAGCAGGAGATCACCCATTACTACGAAGTCGGGGAAGTGGGTGTGGAACACGCCCTCCT
GCCGGAAAAAGGCATCGTCCTGCCGGGCGACCTGGTGATCGGGGCGGACAGCCACACCTGCACCTACGGGGCCCTGGGGG
CCTTCGCGACAGGTGTGGGCAGTACGGACCTGGCGGCGGCCATGCTGACCGGCGAAGTCTGGTTCAAGGTTCCGGAATCC
ATCAGGTTCGTCCTCTCCGGAACGCTCAATCCGTGGGTCTCGGGCAAGGATCTTATCCTTTACATCATCGGTAAGATCGG
CGTGGACGGCGCCCTTTACAAGGCCATGGAATTTACGGGCGACACGATCGCCTCCCTGACCATGGCGGACCGCTTTACCA
TCGCCAACATGGCCATCGAAGCGGGAGGCAAGAACGGCATCTTCACCCCCGATGAAATTACGGAAGCTTATGTGAAGCCC
CGGGCAAAACGCCCCTACACCTTTTACGCCTCCGACCCCGATGCCGCCTACGCCCAGGTCATCGAGATCGATGTGAACCG
GATAGAACCCCAGGTCGCCTTCCCTCATCTGCCCTCCAACACGAAGGGGATCAGCGAGGTGGGCCAGGTCCCGCTCGATC
AGGTCGTCATCGGCTCCTGCACCAACGGACGGATCGAGGATCTGCGGGCAGCGGCCGCCGTGCTGAAAGGACGCAAGGCC
GATCCCCGGGTCCGGCTGATCGTCATCCCGGCAACTCAGGAAATCTATCGAATGGCCATGAAGGAAGGACTCTTCGACAT
CTTCCTTGACGCCAACGCGGCCATCAGCACTCCGACCTGCGGCCCCTGCCTGGGCGGGCATATGGGAATCCTCGCCGCCG
GCGAACGGGCCCTGGCGACGACCAACCGGAATTTCGTCGGCCGGATGGGGCACCCCCGCAGCGAGGTGTATCTGAGCAAT
CCCGCCGTAGCCGCCGCTTCCGCCGTCCTGGGAAGAATTGCCGGCCCTGATGAACTGAAATGA

Upstream 100 bases:

>100_bases
TCAAAGCGCGCTCCGCGTTGGCTTCGTTATCGGTTCGCCGCCCTGCGGTCATCTTTGATCTGGAACTGGAATTAAAATCC
TTTCATGGGAGTGGAAAATC

Downstream 100 bases:

>100_bases
AACTCTCCCGTCTTCCAGAAAACCGTCTCCCCTTTCCGGAGGAGACGGTTTTTTCCGGAAAGACCCAAGACTTCTGAAGG
ATAAAGCGAATGAAATTTAC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MGMTITEKILCRHTDLDEVRPGMLINAKVDIALGNDVTAPLAIDEFRKAGGKKVFDRDKVVLIPDHFTPNKDINSAAQCK
IMRDFAREQEITHYYEVGEVGVEHALLPEKGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGEVWFKVPES
IRFVLSGTLNPWVSGKDLILYIIGKIGVDGALYKAMEFTGDTIASLTMADRFTIANMAIEAGGKNGIFTPDEITEAYVKP
RAKRPYTFYASDPDAAYAQVIEIDVNRIEPQVAFPHLPSNTKGISEVGQVPLDQVVIGSCTNGRIEDLRAAAAVLKGRKA
DPRVRLIVIPATQEIYRMAMKEGLFDIFLDANAAISTPTCGPCLGGHMGILAAGERALATTNRNFVGRMGHPRSEVYLSN
PAVAAASAVLGRIAGPDELK

Sequences:

>Translated_420_residues
MGMTITEKILCRHTDLDEVRPGMLINAKVDIALGNDVTAPLAIDEFRKAGGKKVFDRDKVVLIPDHFTPNKDINSAAQCK
IMRDFAREQEITHYYEVGEVGVEHALLPEKGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGEVWFKVPES
IRFVLSGTLNPWVSGKDLILYIIGKIGVDGALYKAMEFTGDTIASLTMADRFTIANMAIEAGGKNGIFTPDEITEAYVKP
RAKRPYTFYASDPDAAYAQVIEIDVNRIEPQVAFPHLPSNTKGISEVGQVPLDQVVIGSCTNGRIEDLRAAAAVLKGRKA
DPRVRLIVIPATQEIYRMAMKEGLFDIFLDANAAISTPTCGPCLGGHMGILAAGERALATTNRNFVGRMGHPRSEVYLSN
PAVAAASAVLGRIAGPDELK
>Mature_419_residues
GMTITEKILCRHTDLDEVRPGMLINAKVDIALGNDVTAPLAIDEFRKAGGKKVFDRDKVVLIPDHFTPNKDINSAAQCKI
MRDFAREQEITHYYEVGEVGVEHALLPEKGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGEVWFKVPESI
RFVLSGTLNPWVSGKDLILYIIGKIGVDGALYKAMEFTGDTIASLTMADRFTIANMAIEAGGKNGIFTPDEITEAYVKPR
AKRPYTFYASDPDAAYAQVIEIDVNRIEPQVAFPHLPSNTKGISEVGQVPLDQVVIGSCTNGRIEDLRAAAAVLKGRKAD
PRVRLIVIPATQEIYRMAMKEGLFDIFLDANAAISTPTCGPCLGGHMGILAAGERALATTNRNFVGRMGHPRSEVYLSNP
AVAAASAVLGRIAGPDELK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=453, Percent_Identity=28.9183222958057, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=24.5430809399478, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=23.7373737373737, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=33.5470085470085, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI87081781, Length=330, Percent_Identity=27.8787878787879, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=26.3888888888889, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI2367097, Length=462, Percent_Identity=26.1904761904762, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=456, Percent_Identity=28.5087719298246, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=338, Percent_Identity=31.0650887573964, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.9295774647887, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=396, Percent_Identity=26.2626262626263, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=33.5555555555556, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6321429, Length=432, Percent_Identity=32.1759259259259, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323335, Length=449, Percent_Identity=29.3986636971047, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322261, Length=459, Percent_Identity=27.8867102396514, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281365315, Length=457, Percent_Identity=27.5711159737418, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17864292, Length=457, Percent_Identity=27.5711159737418, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571643, Length=445, Percent_Identity=28.5393258426966, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI161076999, Length=420, Percent_Identity=27.1428571428571, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24645686, Length=379, Percent_Identity=27.1767810026385, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137564, Length=379, Percent_Identity=26.1213720316623, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SYNAS (Q2LWJ2)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462618.1
- ProteinModelPortal:   Q2LWJ2
- SMR:   Q2LWJ2
- STRING:   Q2LWJ2
- GeneID:   3883581
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00091
- NMPDR:   fig|56780.10.peg.2563
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   PENTKTI
- PhylomeDB:   Q2LWJ2
- ProtClustDB:   PRK00402
- BioCyc:   SACI56780:SYN_00091-MONOMER
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45100; Mature: 44969

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTITEKILCRHTDLDEVRPGMLINAKVDIALGNDVTAPLAIDEFRKAGGKKVFDRDKV
CCCCHHHHHHHCCCCHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHCCCCEEECCCCE
VLIPDHFTPNKDINSAAQCKIMRDFAREQEITHYYEVGEVGVEHALLPEKGIVLPGDLVI
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEECCCEEE
GADSHTCTYGALGAFATGVGSTDLAAAMLTGEVWFKVPESIRFVLSGTLNPWVSGKDLIL
ECCCCCEEHHHHHHHHHCCCCHHHHHHHHHCHHEEECCHHEEEEEECCCCCCCCCCCEEE
YIIGKIGVDGALYKAMEFTGDTIASLTMADRFTIANMAIEAGGKNGIFTPDEITEAYVKP
EEEEHHCCCHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHCC
RAKRPYTFYASDPDAAYAQVIEIDVNRIEPQVAFPHLPSNTKGISEVGQVPLDQVVIGSC
CCCCCEEEEECCCCCCEEEEEEEEHHHCCCEEECCCCCCCCCCHHHHCCCCCCCEEEECC
TNGRIEDLRAAAAVLKGRKADPRVRLIVIPATQEIYRMAMKEGLFDIFLDANAAISTPTC
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCCCC
GPCLGGHMGILAAGERALATTNRNFVGRMGHPRSEVYLSNPAVAAASAVLGRIAGPDELK
CCCCCCCCEEEECCCEEEEECCCCCEECCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GMTITEKILCRHTDLDEVRPGMLINAKVDIALGNDVTAPLAIDEFRKAGGKKVFDRDKV
CCCHHHHHHHCCCCHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHCCCCEEECCCCE
VLIPDHFTPNKDINSAAQCKIMRDFAREQEITHYYEVGEVGVEHALLPEKGIVLPGDLVI
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEECCCEEE
GADSHTCTYGALGAFATGVGSTDLAAAMLTGEVWFKVPESIRFVLSGTLNPWVSGKDLIL
ECCCCCEEHHHHHHHHHCCCCHHHHHHHHHCHHEEECCHHEEEEEECCCCCCCCCCCEEE
YIIGKIGVDGALYKAMEFTGDTIASLTMADRFTIANMAIEAGGKNGIFTPDEITEAYVKP
EEEEHHCCCHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHCC
RAKRPYTFYASDPDAAYAQVIEIDVNRIEPQVAFPHLPSNTKGISEVGQVPLDQVVIGSC
CCCCCEEEEECCCCCCEEEEEEEEHHHCCCEEECCCCCCCCCCHHHHCCCCCCCEEEECC
TNGRIEDLRAAAAVLKGRKADPRVRLIVIPATQEIYRMAMKEGLFDIFLDANAAISTPTC
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCCCC
GPCLGGHMGILAAGERALATTNRNFVGRMGHPRSEVYLSNPAVAAASAVLGRIAGPDELK
CCCCCCCCEEEECCCEEEEECCCCCEECCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA