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The map label for this gene is leuA

Identifier: 85860415

GI number: 85860415

Start: 2581419

End: 2582969

Strand: Direct

Name: leuA

Synonym: SYN_00090

Alternate gene names: 85860415

Gene position: 2581419-2582969 (Clockwise)

Preceding gene: 85860414

Following gene: 85860416

Centisome position: 81.19

GC content: 58.54

Gene sequence:

>1551_bases
ATGGATTCACCAGAGAAAAGAATTTATATTTTTGATACGACGCTCCGGGATGGGGAACAATCACCCGGTTCGAGCATGAA
CCCCGCGGAAAAACTCCGGATCGCCCGCCAGCTCGAAAAGATGGGGGTCGATATCATCGAAGCCGGCTTCCCCATCGCTT
CCGAAGGCGATTTCCTTTCGGTCCAGCAGATCGCCCAGGAGATCCGCGGCGCCCAGATTGCCGGTCTGGCCAGGGCCAAC
AACGCCGATATCGACCGGGCTTGGGAAGCGATTCGGGACGCGGCCAATCCGCGCATTCACACCTTCATCTCCTCCTCGGA
CATCCATCTGAAATATCAGCTCCGGAAATCCCGGGAGCAGGTCCTGAAGGAGGCCGTCGCCGCCGTCGAAAGGGCGCGGA
GCTATACGCCCAACGTGGAATTTTCCCCCATGGACGCCACCCGCACGGATCGGGGTTATCTCTGCGAAATGGTGGAGGCG
GTCATTGCCGCCGGGGCATCGACGGTCAACATCCCCGATACGGTGGGATACGCCATTCCGCAGGAATTCGGCGAACTGAT
CGCCTATCTTCGTGCCAACGTTCCCAATATTTCCCAGGCGATCATCTCCGTGCACTGCCACAACGATCTCGGACTGGCAG
TGGCCAATTCCCTGTCCGCCATCCTCAACGGCGCCCGGCAGGTCGAATGCACGATCAATGGCATCGGGGAGCGGGCGGGC
AACACGGCCATGGAGGAAGTGGTCATGGCCCTCCGGACGCGCAAAGATCTCTTCGGCTTTTATACGGGCATCAAGACGGA
GAGCATTTATCAGTCCTCCCGTCTGCTGACCCAGATCACGGGCGTCGCGGTACAGCCGAACAAGGCTATCGTCGGGGCGA
ACGCCTTCGCCCACGAATCCGGCATTCATCAGGACGGCCTGATCAAGGAAAAAATCACCTATGAGATCATGACGCCTCAG
TCCGTCGGCATTTCCGACTCCCACATCGTTCTGGGCAAGCATTCCGGTCGTCACGCCGTTTCGGAGCACCTGAAAAAACT
GGGGTTCAACCTGTCCGACACGGAGCTGAACAAGATTTTTGTCCGCTTCAAGGAACTGGCCGACGCCAAAAAGAACGTCT
TCGACGAAGACCTGGAAGCCATTGTCTATGAAGAGCTTTACCGGGTAGAGGACAAATACAAGCTGATCTACCTCAATGTG
GTCAGCGGAAACGTCGCCATCCCCACGGCCACGATGCAGATGGAAGTGGACAGGGAAATCGTCCAGGATGCCGGCTTCGG
TGTCGGTCCGGTGGATGCCACCTTTGACGCGATCCGGAAAATCACGGGCACAAACTACGATCTGCTCCGCTACGTCGTCA
ATGCCATCTCCGGCGGGACGGACGCTCAGGGCGAAGTGACCGTGCAGCTCAAGTTCAACGGCCGCTCCGTCGTGGGGCAC
GGCGCCGACCTCGATGTCATCGTGGCTTCGGCCCGGGCCTACATCAACGCCCTGAACCGCCTGGAGTTCCTCAAGCGGGA
TGCCGGCAAGATCAAATCGGAATACGAATAG

Upstream 100 bases:

>100_bases
CGGTCTTTTCAGGTCAACCGTAAACGAAGAAAAAAAAGAAAAACTGAAAGGCCATGGGCGGAAAGCTCATGGCCTTTTTT
TATCCAGGAAAGGAGCCTTT

Downstream 100 bases:

>100_bases
GCAACATCGTTAAACTTGAATTAATACGCATTTTACGATATCGAGAAAAGCCTGTTCCAATCCCGTTTCAAAGCGCGCTC
CGCGTTGGCTTCGTTATCGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MDSPEKRIYIFDTTLRDGEQSPGSSMNPAEKLRIARQLEKMGVDIIEAGFPIASEGDFLSVQQIAQEIRGAQIAGLARAN
NADIDRAWEAIRDAANPRIHTFISSSDIHLKYQLRKSREQVLKEAVAAVERARSYTPNVEFSPMDATRTDRGYLCEMVEA
VIAAGASTVNIPDTVGYAIPQEFGELIAYLRANVPNISQAIISVHCHNDLGLAVANSLSAILNGARQVECTINGIGERAG
NTAMEEVVMALRTRKDLFGFYTGIKTESIYQSSRLLTQITGVAVQPNKAIVGANAFAHESGIHQDGLIKEKITYEIMTPQ
SVGISDSHIVLGKHSGRHAVSEHLKKLGFNLSDTELNKIFVRFKELADAKKNVFDEDLEAIVYEELYRVEDKYKLIYLNV
VSGNVAIPTATMQMEVDREIVQDAGFGVGPVDATFDAIRKITGTNYDLLRYVVNAISGGTDAQGEVTVQLKFNGRSVVGH
GADLDVIVASARAYINALNRLEFLKRDAGKIKSEYE

Sequences:

>Translated_516_residues
MDSPEKRIYIFDTTLRDGEQSPGSSMNPAEKLRIARQLEKMGVDIIEAGFPIASEGDFLSVQQIAQEIRGAQIAGLARAN
NADIDRAWEAIRDAANPRIHTFISSSDIHLKYQLRKSREQVLKEAVAAVERARSYTPNVEFSPMDATRTDRGYLCEMVEA
VIAAGASTVNIPDTVGYAIPQEFGELIAYLRANVPNISQAIISVHCHNDLGLAVANSLSAILNGARQVECTINGIGERAG
NTAMEEVVMALRTRKDLFGFYTGIKTESIYQSSRLLTQITGVAVQPNKAIVGANAFAHESGIHQDGLIKEKITYEIMTPQ
SVGISDSHIVLGKHSGRHAVSEHLKKLGFNLSDTELNKIFVRFKELADAKKNVFDEDLEAIVYEELYRVEDKYKLIYLNV
VSGNVAIPTATMQMEVDREIVQDAGFGVGPVDATFDAIRKITGTNYDLLRYVVNAISGGTDAQGEVTVQLKFNGRSVVGH
GADLDVIVASARAYINALNRLEFLKRDAGKIKSEYE
>Mature_516_residues
MDSPEKRIYIFDTTLRDGEQSPGSSMNPAEKLRIARQLEKMGVDIIEAGFPIASEGDFLSVQQIAQEIRGAQIAGLARAN
NADIDRAWEAIRDAANPRIHTFISSSDIHLKYQLRKSREQVLKEAVAAVERARSYTPNVEFSPMDATRTDRGYLCEMVEA
VIAAGASTVNIPDTVGYAIPQEFGELIAYLRANVPNISQAIISVHCHNDLGLAVANSLSAILNGARQVECTINGIGERAG
NTAMEEVVMALRTRKDLFGFYTGIKTESIYQSSRLLTQITGVAVQPNKAIVGANAFAHESGIHQDGLIKEKITYEIMTPQ
SVGISDSHIVLGKHSGRHAVSEHLKKLGFNLSDTELNKIFVRFKELADAKKNVFDEDLEAIVYEELYRVEDKYKLIYLNV
VSGNVAIPTATMQMEVDREIVQDAGFGVGPVDATFDAIRKITGTNYDLLRYVVNAISGGTDAQGEVTVQLKFNGRSVVGH
GADLDVIVASARAYINALNRLEFLKRDAGKIKSEYE

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=298, Percent_Identity=22.4832214765101, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1786261, Length=497, Percent_Identity=46.6800804828974, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320071, Length=377, Percent_Identity=29.1777188328912, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=373, Percent_Identity=29.2225201072386, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324225, Length=546, Percent_Identity=25.6410256410256, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=501, Percent_Identity=24.750499001996, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_SYNAS (Q2LWJ3)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462617.1
- ProteinModelPortal:   Q2LWJ3
- SMR:   Q2LWJ3
- STRING:   Q2LWJ3
- GeneID:   3883580
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00090
- NMPDR:   fig|56780.10.peg.2561
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q2LWJ3
- BioCyc:   SACI56780:SYN_00090-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56597; Mature: 56597

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPEKRIYIFDTTLRDGEQSPGSSMNPAEKLRIARQLEKMGVDIIEAGFPIASEGDFLS
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
VQQIAQEIRGAQIAGLARANNADIDRAWEAIRDAANPRIHTFISSSDIHLKYQLRKSREQ
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHH
VLKEAVAAVERARSYTPNVEFSPMDATRTDRGYLCEMVEAVIAAGASTVNIPDTVGYAIP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCHHCH
QEFGELIAYLRANVPNISQAIISVHCHNDLGLAVANSLSAILNGARQVECTINGIGERAG
HHHHHHHHHHHCCCCCHHHHHEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCC
NTAMEEVVMALRTRKDLFGFYTGIKTESIYQSSRLLTQITGVAVQPNKAIVGANAFAHES
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCC
GIHQDGLIKEKITYEIMTPQSVGISDSHIVLGKHSGRHAVSEHLKKLGFNLSDTELNKIF
CCCCCCCCHHHEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHH
VRFKELADAKKNVFDEDLEAIVYEELYRVEDKYKLIYLNVVSGNVAIPTATMQMEVDREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEECCEEEEHHHHHHH
VQDAGFGVGPVDATFDAIRKITGTNYDLLRYVVNAISGGTDAQGEVTVQLKFNGRSVVGH
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEC
GADLDVIVASARAYINALNRLEFLKRDAGKIKSEYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDSPEKRIYIFDTTLRDGEQSPGSSMNPAEKLRIARQLEKMGVDIIEAGFPIASEGDFLS
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
VQQIAQEIRGAQIAGLARANNADIDRAWEAIRDAANPRIHTFISSSDIHLKYQLRKSREQ
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHH
VLKEAVAAVERARSYTPNVEFSPMDATRTDRGYLCEMVEAVIAAGASTVNIPDTVGYAIP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCHHCH
QEFGELIAYLRANVPNISQAIISVHCHNDLGLAVANSLSAILNGARQVECTINGIGERAG
HHHHHHHHHHHCCCCCHHHHHEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCC
NTAMEEVVMALRTRKDLFGFYTGIKTESIYQSSRLLTQITGVAVQPNKAIVGANAFAHES
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCC
GIHQDGLIKEKITYEIMTPQSVGISDSHIVLGKHSGRHAVSEHLKKLGFNLSDTELNKIF
CCCCCCCCHHHEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHH
VRFKELADAKKNVFDEDLEAIVYEELYRVEDKYKLIYLNVVSGNVAIPTATMQMEVDREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEECCEEEEHHHHHHH
VQDAGFGVGPVDATFDAIRKITGTNYDLLRYVVNAISGGTDAQGEVTVQLKFNGRSVVGH
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEC
GADLDVIVASARAYINALNRLEFLKRDAGKIKSEYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA