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The map label for this gene is nifU [H]

Identifier: 85860379

GI number: 85860379

Start: 2552876

End: 2553565

Strand: Direct

Name: nifU [H]

Synonym: SYN_01182

Alternate gene names: 85860379

Gene position: 2552876-2553565 (Clockwise)

Preceding gene: 85860378

Following gene: 85860380

Centisome position: 80.3

GC content: 51.88

Gene sequence:

>690_bases
ATGTGGGATTATACGGATAAGGTCAGGGAGCATTTCTGGAATCCCCGCAACGTGGGGGAAGTTGAAAATCCTGACGGTAT
TGGTCAGGTCGGTTCCCTTGCCTGTGGCGACGCGCTCCGCCTTACATTCAAACTGGATGAAAACAAAAGGATCAAAGAAG
CGAAATTCAAGAGTTTCGGCTGTGCCAGCGCTATTGCCGCGGCGTCGGTCCTGACGGAAATGGTCGTGGGCCTTACGATC
GAACAGGCGGAAAAAATCACGAATCAGGACATTGCCGCCGCCCTTGGAGGACTGCCTGAACAAAAGATGCACTGTTCCGT
TCTCGGGGAACAGGTTCTTGAAAAAGCGATTGCCTGTTATCGAGGGCGCCCGGCAGGCGCAAAAGAAGAAAAGATGATCT
GTGAATGTTTCAGCGTAACGGATCGGGAAATCGAGAAAGCCGTCAGGGAGAACCGTCTTACTTCGGTGGAACAGGTAACC
AACTACACGAAAGTGGGAGGCGGCTGTGGAAACTGCCACGGGAGGATTCAGGAGATTCTTGACTGGGAGCGAACCGAATT
CCGCAAGTCCGTCCGCCCGAAATTAATCGATATCCGGACAATCAGCAGAATACAGGATAAACCAGAAACCGGAAACTGGG
AGAAATCAGGGAATCAATTCCCGAGCGGACAGGACAAAATCAACCCCTGA

Upstream 100 bases:

>100_bases
CCTGGGAGAATACGGCCGAAATGTAAACAGGGCAAGGGGATAAACGCATCGACCAGAGAGAAGACAAGAATCGGAAAGGC
ATAGATAAATAAATAGAACC

Downstream 100 bases:

>100_bases
GATGAGTAAAGATATTGAAGATCAGACGCAGATCATTCTTGACAGCATTGCAGATGGCGTTTTTACCGTTGATTCTGAAT
GGAAAATCACATCTTTCAAC

Product: NifU domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MWDYTDKVREHFWNPRNVGEVENPDGIGQVGSLACGDALRLTFKLDENKRIKEAKFKSFGCASAIAAASVLTEMVVGLTI
EQAEKITNQDIAAALGGLPEQKMHCSVLGEQVLEKAIACYRGRPAGAKEEKMICECFSVTDREIEKAVRENRLTSVEQVT
NYTKVGGGCGNCHGRIQEILDWERTEFRKSVRPKLIDIRTISRIQDKPETGNWEKSGNQFPSGQDKINP

Sequences:

>Translated_229_residues
MWDYTDKVREHFWNPRNVGEVENPDGIGQVGSLACGDALRLTFKLDENKRIKEAKFKSFGCASAIAAASVLTEMVVGLTI
EQAEKITNQDIAAALGGLPEQKMHCSVLGEQVLEKAIACYRGRPAGAKEEKMICECFSVTDREIEKAVRENRLTSVEQVT
NYTKVGGGCGNCHGRIQEILDWERTEFRKSVRPKLIDIRTISRIQDKPETGNWEKSGNQFPSGQDKINP
>Mature_229_residues
MWDYTDKVREHFWNPRNVGEVENPDGIGQVGSLACGDALRLTFKLDENKRIKEAKFKSFGCASAIAAASVLTEMVVGLTI
EQAEKITNQDIAAALGGLPEQKMHCSVLGEQVLEKAIACYRGRPAGAKEEKMICECFSVTDREIEKAVRENRLTSVEQVT
NYTKVGGGCGNCHGRIQEILDWERTEFRKSVRPKLIDIRTISRIQDKPETGNWEKSGNQFPSGQDKINP

Specific function: Involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=121, Percent_Identity=47.1074380165289, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI24307953, Length=122, Percent_Identity=45.9016393442623, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788878, Length=123, Percent_Identity=45.5284552845528, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17543474, Length=123, Percent_Identity=46.3414634146341, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=46.7741935483871, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324800, Length=124, Percent_Identity=43.5483870967742, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355597, Length=121, Percent_Identity=49.5867768595041, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 25532; Mature: 25532

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDYTDKVREHFWNPRNVGEVENPDGIGQVGSLACGDALRLTFKLDENKRIKEAKFKSFG
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHCC
CASAIAAASVLTEMVVGLTIEQAEKITNQDIAAALGGLPEQKMHCSVLGEQVLEKAIACY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RGRPAGAKEEKMICECFSVTDREIEKAVRENRLTSVEQVTNYTKVGGGCGNCHGRIQEIL
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DWERTEFRKSVRPKLIDIRTISRIQDKPETGNWEKSGNQFPSGQDKINP
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MWDYTDKVREHFWNPRNVGEVENPDGIGQVGSLACGDALRLTFKLDENKRIKEAKFKSFG
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHCC
CASAIAAASVLTEMVVGLTIEQAEKITNQDIAAALGGLPEQKMHCSVLGEQVLEKAIACY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RGRPAGAKEEKMICECFSVTDREIEKAVRENRLTSVEQVTNYTKVGGGCGNCHGRIQEIL
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DWERTEFRKSVRPKLIDIRTISRIQDKPETGNWEKSGNQFPSGQDKINP
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672; 7947754; 10819462 [H]